2018
DOI: 10.1038/s41467-018-05403-1
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Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

Abstract: Many intrinsically disordered proteins self-assemble into liquid droplets that function as membraneless organelles. Because of their biological importance and ability to colocalize molecules at high concentrations, these protein compartments represent a compelling target for bio-inspired materials engineering. Here we manipulated the intrinsically disordered, arginine/glycine-rich RGG domain from the P granule protein LAF-1 to generate synthetic membraneless organelles with controllable phase separation and ca… Show more

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Cited by 357 publications
(426 citation statements)
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“…Germ granules in Caenorhabditis elegans (P granules) were the first BioMCs whose condensation and dissolution behavior was compared to liquids and characterized in vivo as flowing, deforming, coalescing, as well as fusion and fission of individual protein‐rich droplets . Similar liquid‐like properties have also been observed for highly concentrated preparations of proteins or RNAs in vitro . The process that influences the properties of fluids with different densities (i.e., oil‐in‐water emulsions) and governs their behavior is called liquid–liquid de‐mixing or LLPS, which has gained a lot of attention recently.…”
Section: Dynamic Liquid–liquid Phase Separation Is Key For Proper Celmentioning
confidence: 99%
See 1 more Smart Citation
“…Germ granules in Caenorhabditis elegans (P granules) were the first BioMCs whose condensation and dissolution behavior was compared to liquids and characterized in vivo as flowing, deforming, coalescing, as well as fusion and fission of individual protein‐rich droplets . Similar liquid‐like properties have also been observed for highly concentrated preparations of proteins or RNAs in vitro . The process that influences the properties of fluids with different densities (i.e., oil‐in‐water emulsions) and governs their behavior is called liquid–liquid de‐mixing or LLPS, which has gained a lot of attention recently.…”
Section: Dynamic Liquid–liquid Phase Separation Is Key For Proper Celmentioning
confidence: 99%
“…The principles and forces governing LLPS are currently extensively studied, not only by in vitro assays using purified BioMC components but also by using in vivo model systems . While many constituent molecules of particular BioMCs have already been identified, and both overexpression as well as genetic deletion approaches of proteins and RNAs are being used to modulate the formation and dynamics of BioMCs, it still remains largely unclear which signals and specific factors trigger the separation of membrane‐less macromolecular assemblies within complex environments.…”
Section: Dynamic Liquid–liquid Phase Separation Is Key For Proper Celmentioning
confidence: 99%
“…[3,4] Microdroplets produced in vitro by associative (coacervates) or segregative (aqueous two-phase systems) liquid-liquid phase separation have recently been used as synthetic models of dynamic protocells. [15,[29][30][31][32] However,p latforms enabling the rapid and localized actuation of polynucleotide microphase separation have not yet been developed. [14] Polynucleotides have been used as scaffold components to assemble coacervate droplets, [15][16][17][18][19] and selective sequestration of guest polynucleotides has been demonstrated in preformed coacervates.…”
mentioning
confidence: 99%
“…[12,20,21] Owing to their liquid-like nature and the weak molecular interactions,s ynthetic coacervate droplets can dynamically respond to external stimuli. [15,[29][30][31][32] However,p latforms enabling the rapid and localized actuation of polynucleotide microphase separation have not yet been developed. [15,[29][30][31][32] However,p latforms enabling the rapid and localized actuation of polynucleotide microphase separation have not yet been developed.…”
mentioning
confidence: 99%
“…Controls are shown with light omitted (columns 2 and 4) or CRY omitted (columns [3][4]. Table values reflect the fraction of cells with high Citrine intensity, i.e., cells in the upper FACS quadrants Q1 and Q2 (quadrants are defined in (B)).…”
Section: B Facs Analysis Of Yeast Library 6 Hours After 8-minute Blumentioning
confidence: 99%