Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development

Dolega, Monika E.; Abeille, Fabien; Picollet-D’hahan, Nathalie; Gidrol, Xavier

doi:10.1016/j.biomaterials.2015.02.042

Cited by 73 publications

(75 citation statements)

References 55 publications

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“…According to Fig. 8(E–F), single prostate epithelial cell in matrigel bead is more likely to form lumen rather than cellular spheroid over 7-day culture 198 . The active-caspase-3 immunostaining in Fig.…”

Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 97%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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Hydrogel microcapsules provide miniaturized and biocompatible niches for three-dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based microfluidics with tunable size, morphology, and biochemical properties. Therefore, microfluidic generation and manipulation of cell-laden microcapsules can be used for 3D cell culture to mimic the in vivo environment towards applications in tissue engineering and high throughput drug screening. In this review of recent advances mainly since 2010, we will first introduce general characteristics of droplet-based microfluidic devices for cell encapsulation with an emphasis on the fluid dynamics of droplet breakup and internal mixing as they directly influence microcapsule’s size and structure. We will then discuss two on-chip manipulation strategies: sorting and extraction from oil into aqueous phase, which can be integrated into droplet-based microfluidics and significantly improve the qualities of cell-laden hydrogel microcapsules. Finally, we will review various applications of hydrogel microencapsulation for 3D in vitro culture on cell growth and proliferation, stem cell differentiation, tissue development, and co-culture of different types of cells.

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Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 97%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

et al. 2017

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“…Matrigel is a more realistic model to mimic the ECM, even though it suffers of a lack of control in terms of composition, structure and reproducibility. Here we follow the compression of a Matrigel bead, made following the method recently published by Dolega et al [22] and submitted to the osmotic stress. To facilitate the measurement, the beads are doped with fluorescent nanoparticles.…”

Section: Matrigel Compressionmentioning

confidence: 99%

Effect of an osmotic stress on multicellular aggregates

Monnier

Delarue

Brunel

et al. 2016

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“…Similar microfluidic schemes for producing microgels [14][15][16] and encapsulating cells [17][18][19] have recently been reported for widely varying applications, including wound healing, stem cell culture and fundamental studies of cell biology. The versatility of microfluidic cell encapsulation extends to other polymers, including Matrigel 20 , agarose 21 and even multilayer core-shell composites such as collagen cores with alginate shells 22 . Minimization of microgel diameter maximizes transport of oxygen and nutrients to encapsulated cells, enables applications for which larger microgels are not suited (such as injection) and potentially reduces transplant graft volume.…”

Section: Introductionmentioning

confidence: 99%

Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation

2018

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Cells can be microencapsulated in synthetic hydrogel microspheres (microgels) using droplet microfluidics, but microfluidic devices with a single droplet generating geometry have limited throughput, especially as microgel diameter decreases. Here we demonstrate microencapsulation of human mesenchymal stem cells (hMSCs) in small ( o100 μm diameter) microgels utilizing parallel droplet generators on a two-layer elastomer device, which has 600% increased throughput vs. single-nozzle devices. Distribution of microgel diameters were compared between products of parallel vs. single-nozzle configurations for two square nozzle widths, 35 and 100 μm. Microgels produced on parallel nozzles were equivalent to those produced on single nozzles, with substantially the same polydispersity. Microencapsulation of hMSCs was compared for parallel nozzle devices of each width. Thirty five micrometer wide nozzle devices could be operated at twice the cell concentration of 100 μm wide nozzle devices but produced more empty microgels than predicted by a Poisson distribution. Hundred micrometer wide nozzle devices produced microgels as predicted by a Poisson distribution. Polydispersity of microgels did not increase with the addition of cells for either nozzle width. hMSCs encapsulated on 35 μm wide nozzle devices had reduced viability (~70%) and a corresponding decrease in vascular endothelial growth factor (VEGF) secretion compared to hMSCs cultured on tissue culture (TC) plastic. Encapsulating hMSCs using 100 μm wide nozzle devices mitigated loss of viability and function, as measured by VEGF secretion.

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Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development

Cited by 73 publications

References 55 publications

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Effect of an osmotic stress on multicellular aggregates

Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation

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