Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts

Abstract

International audiencePoly(oligoethylene glycol methacrylate), POEGMA, brushes were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated silicon wafers. Prior to ATRP, the substrates were grafted by brominated aryl initiators via the electrochemical reduction of a noncommercial parent diazonium salt of the formula BF4-, N-+(2)-C6H4-CH(CH3)Br. The diazonium-modified gold plates (Au-Br) served as macroinitiators for ATRP of OEGMA which resulted in hydrophilic surfaces (Au-POEGMA) that could be used for two distinct objectives: (i) resistance to fouling by Salmonella Typhimurium; (ii) specific recognition of the same bacteria provided that the POEGMA grafts are activated by anti-Salmonella. The Au-POEGMA plates were characterized by XPS, polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) and contact angle measurements. Both Beer-Lambert equation and Tougaard's QUASES software indicated a POEGMA thickness that exceeds the critical similar to 10 nm value necessary for obtaining a hydrophilic polymer with effective resistance to cell adhesion. The Au-POEGMA slides were further activated by trichlorotriazine (TCT) in order to covalently bind anti-Salmonella antibodies (AS). The antibody-modified Au-POEGMA specimens were found to specifically attach Salmonella Typhimurium bacteria. This work is another example of the diazonium salt/ATRP process to provide biomedical polymer surfaces

Anti-biofouling ability and cytocompatibility of the zwitterionic brushes-modified cellulose membrane

“…Similar trends were observed on other substrates that underwent the same initiator immobilization process for SI-ATRP. 45,46 As expected, the water contact angles sharply decreased to 20.3 aer the three zwitterionic brushes were graed onto the CM surfaces, even being statistically lower than that of the pristine CM. This is because the zwitterionic brushes (zwitterionic motifs) can give an ionic structure to water molecules in addition to the hydrogen bonding, thereby generating a super-hydrophilic surface.…”

Aryl diazonium salts: a new class of coupling agents for bonding polymers, biomacromolecules and nanoparticles to surfaces

“…4.1.4 Antibody-functionalized polymer brushes for bacterial sensing. Instead of binding antibodies to the aryl layers as reported above, Mrabet et al 72 have attached antibodies to hydrophilic POEGMA grafts generated by diazoniuminitiated ATRP on gold (Au-POEGMA). This was achieved through the TCT coupling procedure.…”

Diazonium salts for surface-confined visible light radical photopolymerization

“…The average values are in line with the literature for PMMA grafts but slightly higher for PHEMA probably due to a slight contribution of DMA as gold is detected by XPS through the PHEMA top layer and thus through also DMA. For UV/ozone cleaned Au plate, the water contact angle was reported to be 35.5° while for aryl layer on various substrates the contact angle is in the 55° to 100° range . Interestingly, for each polymer the use of the crosslinker imparts a slight degree of relative hydrophilicity.…”