Controlled branching of polyglycidol and formation of protein–glycidol bioconjugates via a graft-from approach with “PEG-like” arms

Spears, Benjamin R.; Waksal, Julian A.; McQuade, Caitlin; Lanier, Laura; Harth, Eva

doi:10.1039/c3cc38369e

Cited by 38 publications

(43 citation statements)

References 17 publications

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“…, ▪) with temperature was observed for P[(VA) ‐g‐ (hPG)] (Table S4). This is in contrast to the work on the synthesis of a hPG homopolymer carried out by Harth and coworkers which displayed a temperature dependence . The difference in temperature dependence could be attributed to their smaller initiator (isoamyl alcohol), different catalyst (tin[II] trifluoromethanesulfonate), or the short chain lengths of the grafted hPG chains in P[(VA) ‐g‐ (hPG)] prepared here were insufficient for this trend to be observed.…”

Section: Resultscontrasting

confidence: 95%

“…The hyperbranched structure results in increased hydroxyl functionality and therefore polymers with greater %DB will have greater hydroxyl content. Harth and coworkers polymerized glycidol using isoamyl alcohol as the initiator and tin octoate as the catalyst and have shown that temperature can affect the %DB of hPG, as the %DB decreased from 24% at 40 °C to 15% at −15 °C …”

Section: Introductionmentioning

confidence: 99%

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Synthesis of poly(vinyl alcohol‐graft‐hyperbranched glycerol)

King

Khosravi

Musa

2017

J. Polym. Sci. Part A: Polym. Chem.

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We report the first example of grafting hyperbranched polyglycerol onto poly(vinyl alcohol) via ring-opening polymerisation of glycidol to prepare poly(vinyl alcohol-graft-hyperbranched glycerol) (P[(VA)-g-(hPG)]). The effects of catalyst, molecular weight of PVA, reaction temperature, water content, moles of reagent, and addition time of reagent were also investigated. P[(VA)-g-(hPG)] with various mole fractions of hPG were prepared and the degrees of substitution and branching were determined. P[(VA)-g-(hPG)] displayed decreased degree of crystallinity and also increased solubility in water, compared to PVA. P[(VA)-g-(hPG)] is shown to be a superior hair styling polymer with a curl retention value of 85% after 4 h.

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Section: Resultscontrasting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Synthesis of poly(vinyl alcohol‐graft‐hyperbranched glycerol)

King

Khosravi

Musa

2017

J. Polym. Sci. Part A: Polym. Chem.

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“…A conjugate with midfunctional PG demonstrated significantly higher activity than its linear counterpart at a similar MW [26]. Harth and coworkers were the first to demonstrate in situ grafting of branched PG from Bovine Serum Albumin (BSA) using Ring Opening Polymerization (ROP) with the ability to control the degree of branching [28]. …”

Section: Non-degradable Peg Alternativesmentioning

confidence: 99%

Protein–polymer conjugation — moving beyond PEGylation

Chilkoti

2015

Current Opinion in Chemical Biology

156

120

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In this review, we summarize —from a materials science perspective— the current state of the field of polymer conjugates of peptide and protein drugs, with a focus on polymers that have been developed as alternatives to the current gold standard, poly(ethylene glycol) (PEG). PEGylation, or the covalent conjugation of PEG to biological therapeutics to improve their therapeutic efficacy by increasing their circulation half-lives and stability, has been the gold standard in the pharmaceutical industry for several decades. After years of research and development, the limitations of PEG, specifically its non-degradability and immunogenicity have become increasingly apparent. While PEG is still currently the best polymer available with the longest clinical track record, extensive research is underway to develop alternative materials in an effort to address these limitations of PEG. Many of these alternative materials have shown promise, though most of them are still in an early stage of development and their in vivo distribution, mechanism of degradation, route of elimination and immunogenicity have not been investigated to a similar extent as for PEG. Thus, further in-depth in vivo testing is essential to validate whether any of the alternative materials discussed in this review qualify as a replacement for PEG.

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“…The graft polymerization of GL from SiO 2 NP-PCL-OH (prepared in Section 2.3) using tin triflate was performed by modifying the method reported in the literature [26]. SiO 2 NP-PCL-OH (500 mg) was dispersed by sonication in 25 mL of anhydrous anisole in a 100 mL Schlenk flask.…”

Section: Sirop Of Gl From the Pcl Grafted Silica Nanoparticles (Sio 2mentioning

confidence: 99%