Controlled Co-delivery of Growth Factors through Layer-by-Layer Assembly of Core–Shell Nanofibers for Improving Bone Regeneration

Cheng, Gu; Yin, Chengcheng; Tu, Hu; Jiang, Shan; Wang, Qun; Zhou, Xue; Xing, Xin; Xie, Congyong; Shi, Xiaowen; Du, Yuming; Deng, Hongbing; Li, Zubing

doi:10.1021/acsnano.8b06032

Cited by 215 publications

(147 citation statements)

References 36 publications

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“…It is worth noting that the sequential release of various growth factors from the electrospun nanofibers could accelerate vascularized bone formation. Accordingly, Cheng et al fabricated multilayer core-shell silk fibroin (SF)/PCL/PVA nanofibrous scaffolds containing BMP2 (10 µg/mL) and connective tissue growth factor (CTGF) (10 µg/mL) [246]. For this aim, they incorporated BMP2 into the core of the mats by applying coaxial electrospinning and then immobilized CTGF onto their surface via a layer-by-layer (LBL) self-assembly technique (Figure 3).…”

Section: Angiogenic Nanofibers For Hard Tissue Engineeringmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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Angiogenesis (or the development of new blood vessels) is a key event in tissue engineering and regenerative medicine; thus, a number of biomaterials have been developed and combined with stem cells and/or bioactive molecules to produce three-dimensional (3D) pro-angiogenic constructs. Among the various biomaterials, electrospun nanofibrous scaffolds offer great opportunities for pro-angiogenic approaches in tissue repair and regeneration. Nanofibers made of natural and synthetic polymers are often used to incorporate bioactive components (e.g., bioactive glasses (BGs)) and load biomolecules (e.g., vascular endothelial growth factor (VEGF)) that exert pro-angiogenic activity. Furthermore, seeding of specific types of stem cells (e.g., endothelial progenitor cells) onto nanofibrous scaffolds is considered as a valuable alternative for inducing angiogenesis. The effectiveness of these strategies has been extensively examined both in vitro and in vivo and the outcomes have shown promise in the reconstruction of hard and soft tissues (mainly bone and skin, respectively). However, the translational of electrospun scaffolds with pro-angiogenic molecules or cells is only at its beginning, requiring more research to prove their usefulness in the repair and regeneration of other highly-vascularized vital tissues and organs. This review will cover the latest progress in designing and developing pro-angiogenic electrospun nanofibers and evaluate their usefulness in a tissue engineering and regenerative medicine setting.

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Section: Angiogenic Nanofibers For Hard Tissue Engineeringmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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“…Most available reports have focused on immobilizing a single GF to be investigated as soluble or immobilized in a complex medium. Nonetheless, the functionalization of different surfaces with multiple GFs has also been implemented using both chemical and physical approaches (Stefonek-Puccinelli and Masters, 2008;Shah et al, 2011;Banks et al, 2014;Lequoy et al, 2016;Mao et al, 2017;Cheng et al, 2019). Importantly, the synergistic or antagonistic effect that coimmobilized GFs may have on bioactivity strongly depends on the nature of GFs and their concentrations in culture, so that no generalization can be made about the immobilization of more than a single GF (Stefonek-Puccinelli and Masters, 2008;Banks et al, 2014;Mao et al, 2017).…”

Section: Methods For Gfs Immobilizationmentioning

confidence: 99%

Immobilization of Growth Factors for Cell Therapy Manufacturing

Enriquez-Ochoa

Robles-Ovalle

Mayolo‐Deloisa

et al. 2020

Front. Bioeng. Biotechnol.

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Cell therapy products exhibit great therapeutic potential but come with a deterring price tag partly caused by their costly manufacturing processes. The development of strategies that lead to cost-effective cell production is key to expand the reach of cell therapies. Growth factors are critical culture media components required for the maintenance and differentiation of cells in culture and are widely employed in cell therapy manufacturing. However, they are expensive, and their common use in soluble form is often associated with decreased stability and bioactivity. Immobilization has emerged as a possible strategy to optimize growth factor use in cell culture. To date, several immobilization techniques have been reported for attaching growth factors onto a variety of biomaterials, but these have been focused on tissue engineering. This review briefly summarizes the current landscape of cell therapy manufacturing, before describing the types of chemistry that can be used to immobilize growth factors for cell culture. Emphasis is placed to identify strategies that could reduce growth factor usage and enhance bioactivity. Finally, we describe a case study for stem cell factor.

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“…Co-axial electrospinning is a two-stream process that results in the fabrication of multipolymer fibers with the inner stream being the "core, " and the outer polymer passed stream forms the shell (Jiang et al, 2014). This method is auspicious for the incorporation of fragile cargos (e.g., DNA or growth factors) as the therapeutic interaction with the shell polymer blend which may be produced with harsh solvents is minimized, therefore preserving the cargo (Ghosh et al, 2008;Xie et al, 2016;Cheng et al, 2019). Wei et al utilized this technique for the development of a wound dressing, comprising a PCL core and collagen shell nanofibers.…”

Section: Electrospinningmentioning

confidence: 99%

Electrospun Biomaterials in the Treatment and Prevention of Scars in Skin Wound Healing

Mulholland

2020

Front. Bioeng. Biotechnol.

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Electrospinning is a promising method for the rapid and cost-effective production of nanofibers from a wide variety of polymers given the high surface area morphology of these nanofibers, they make excellent wound dressings, and so have significant potential in the prevention and treatment of scars. Wound healing and the resulting scar formation are exceptionally well-characterized on a molecular and cellular level. Despite this, novel effective anti-scarring treatments which exploit this knowledge are still clinically absent. As the process of electrospinning can produce fibers from a variety of polymers, the treatment avenues for scars are vast, with therapeutic potential in choice of polymers, drug incorporation, and cell-seeded scaffolds. It is essential to show the new advances in this field; thus, this review will investigate the molecular processes of wound healing and scar tissue formation, the process of electrospinning, and examine how electrospun biomaterials can be utilized and adapted to wound repair in the hope of reducing scar tissue formation and conferring an enhanced tensile strength of the skin. Future directions of the research will explore potential novel electrospun treatments, such as gene therapies, as targets for enhanced tissue repair applications. With this class of biomaterial gaining such momentum and having such promise, it is necessary to refine our understanding of its process to be able to combine this technology with cutting-edge therapies to relieve the burden scars place on world healthcare systems.

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Controlled Co-delivery of Growth Factors through Layer-by-Layer Assembly of Core–Shell Nanofibers for Improving Bone Regeneration

Cited by 215 publications

References 36 publications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Immobilization of Growth Factors for Cell Therapy Manufacturing

Electrospun Biomaterials in the Treatment and Prevention of Scars in Skin Wound Healing

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