Controlled dehydration of a biological sample using an alternative form of environmental SEM

Nedĕla,

doi:10.1111/j.1365-2818.2009.03216.x

Cited by 31 publications

(25 citation statements)

References 12 publications

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“…Already-fixed samples (70% ethanol or 4% formaldehyde solution) were washed and rinsed with a drop of distilled water. Observations were made using the experimental ESEM AQUASEM II (Neděla, 2010) equipped with an ionization detector of secondary electrons, a specially designed hydration system and Peltier cooled specimen holder. A unique method for reaching and keeping thermodynamic conditions was used (Tihlaříková, Neděla, & Shiojiri, 2013).…”

Section: Sample Preparation and Observing Conditionsmentioning

confidence: 99%

“…SEM condemns samples to be destroyed so that further analysis is not possible (e.g., for molecular study or depositing as type material in a museum). Environmental scanning electron microscopy (ESEM) based on new methods (Neděla 2010;Neděla et al, 2015) and instrumentation (Jirák et al, 2010) can be used for the advanced study of parasites in their native state. It was shown by Tihlaříková, Neděla, and Shiojiri (2013) in a study of surviving mites.…”

Section: Introductionmentioning

confidence: 99%

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Ciscem 2014

Jonge

2015

Advances in Imaging and Electron Physics

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Section: Sample Preparation and Observing Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ciscem 2014

Jonge

2015

Advances in Imaging and Electron Physics

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“…It was designed in the Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic for research on detection systems and ESEM techniques [15]. The single crystal YAG:Ce 3+ , used for detection of backscattered electrons (BSEs) in high and low vacuum conditions, has a hole in the center so that it simultaneously acts as a pressure-limiting aperture of the AQUASEM II [14].…”

Section: Observation In Esemmentioning

confidence: 99%

Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM

2015

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Environmental scanning electron microscopy enables the investigation of uncoated pine early embryogenic tissue samples in situ. The samples were examined under low vacuum conditions (air pressure 550 Pa) at a temperature of around -18°C by the AQUASEM II noncommercial environmental scanning electron microscope. The native extracellular matrix surface network was imaged by the environmental scanning electron microscope and in dark field mode of the optical microscope too. The backscattered electron detector disclosed brightness loci in the cells of early embryogenic culture. This work shows images of native pine embryogenic tissues. The continuity of extracellular matrix with structural integrity of plant organism is discussed.

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“…ESEM AQUASEM II equipped with a YAG:Ce 3+ BSEs detector, an ionization detector of SEs, a special hydration system and a Peltier cooled stage were used [1]. The pressures between 400-700 Pa, 50% water-vapor saturation, and the temperatures above 250 K were utilized in the experiments.…”

Section: +mentioning

confidence: 99%

“…A uranyl nitrate solution (0.01 M) acidified by perchloric acid to pH = 1 was used because hydrolysis of UO 2 2+ is suppressed, and only a single species (i.e., a hydrated uranyl ion) is present under these conditions. ESEM AQUASEM II equipped with a YAG:Ce 3+ BSEs detector, an ionization detector of SEs, a special hydration system and a Peltier cooled stage were used [1]. The pressures between 400-700 Pa, 50% water-vapor saturation, and the temperatures above 250 K were utilized in the experiments.…”

mentioning

confidence: 99%

Morphological and Chemical Analysis of Impurities in Ice Using the Environmental Scanning Electron Microscopy and Fluorescence Spectroscopy

Neděla¹,

Runštuk²,

Krausko

et al. 2015

Microsc Microanal

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Accumulated and concentrated impurities can be stored in natural ice or snow. They are found to be rejected from the freezing solution to the ice grain boundaries, free ice surfaces or liquid/brine inclusions. Information about compartmentation and phase speciation in ice is thus essential for the assessment of their fate. The location of impurities and their interactions with the water molecules of ice, still not sufficiently clarified, must be studied at low temperatures because thawing smears the information out. When the impurities keep their location while the surrounding ice sublimes, a 3D morphology of the ice boundaries is revealed. Environmental scanning electron microscopy (ESEM) is one of the few methods allowing direct observation of ice bulk sample with location and compartmentation impurities in dynamically changing conditions of relatively high pressure of gas and stable temperature of cooled sample holder.For this work, aqueous (aq) samples were frozen under atmospheric pressure on a silicon plate cooled by the Peltier stage. The initial sample holder temperature was above -1 °C, and a droplet of pure water or the uranyl nitrate solution placed on it was frozen. A uranyl nitrate solution (0.01 M) acidified by perchloric acid to pH = 1 was used because hydrolysis of UO 2 2+ is suppressed, and only a single species (i.e., a hydrated uranyl ion) is present under these conditions. ESEM AQUASEM II equipped with a YAG:Ce 3+ BSEs detector, an ionization detector of SEs, a special hydration system and a Peltier cooled stage were used [1]. The pressures between 400-700 Pa, 50% water-vapor saturation, and the temperatures above 250 K were utilized in the experiments. The phenomena of etching and subsequent stripping of impurities are largely suppressed at these conditions. In order to get information about the phase speciation of the uranyl ion and its microenvironment in the ice samples, the corresponding frozen aq solutions were subjected to a luminescence analysis. Crystalline uranyl nitrate hexahydrate provided a luminescence emission spectrum with the emission band maxima located at 488, 509, 533, 559, and 587 nm at 293 K (Fig. 1a). A hydrated uranyl ion in a solution exhibited one additional band at 473 nm besides those observed in the emission spectrum of a crystal. The luminescence lifetime of crystalline uranyl nitrate is known to depend considerably on the degree of its hydration. Uranyl perchlorate was prepared from solid uranyl nitrate by repeated cycles of dissolution in perchloric acid (70%) and evaporation. The luminescence lifetime of uranyl perchlorate crystals was found to be (283 ± 10) μs. In addition, the dependence of the uranyl ion luminescence lifetime on the perchloric acid concentration has been reported to be nearly linear at the concentrations between 0.3 and 10 M (0.1 M aq HClO 4 : τ = 2 μs; 11 M aq HClO 4 : τ = 65 μs) [2]. We utilized the linear regression equation to estimate the perchloric acid concentration in the brine.In this work, the mono-exponential lifetime of uranyl a...

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Controlled dehydration of a biological sample using an alternative form of environmental SEM

Cited by 31 publications

References 12 publications

Ciscem 2014

Ciscem 2014

Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM

Morphological and Chemical Analysis of Impurities in Ice Using the Environmental Scanning Electron Microscopy and Fluorescence Spectroscopy

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