Controlled dermal cell damage as human in vivo model for localised pain and inflammation

Fairweather, I.; McGlone, Francis; Reilly, David; Rukwied, Roman

doi:10.1007/s00011-003-1234-0

Cited by 10 publications

(4 citation statements)

References 60 publications

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“…Topical SLS did not elicit spontaneous pain. In comparison, infusion of SLS into the skin via microdialysis fibers has been shown to induce immediate pain and release of prostaglandin E 2 [23], likely due to either a direct toxic effect or activation of acid-sensing nociceptors by the low pH of most SLS solutions. Here, we show that SLS induces significant, dose-related thermal and tactile hyperalgesia.…”

Section: Discussionmentioning

confidence: 99%

A novel model of inflammatory pain in human skin involving topical application of sodium lauryl sulfate

2010

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Section: Discussionmentioning

confidence: 99%

A novel model of inflammatory pain in human skin involving topical application of sodium lauryl sulfate

2010

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“…Charged surfactants, such as sodium dodecyl sulfate (used in PRO-LEUKIN , manufactured by Novartis) may also be effective in reducing HMWS in reconstituted lyophilized products. Although approved for use parenterally, anionic surfactants can lyse cells at high concentrations, causing stinging and pain upon injection [100,101]. Therefore, thorough preclinical testing must be done to ensure adequate safety in utilizing these anionic surfactants in lyophilized formulations.…”

Section: Surfactantsmentioning

confidence: 98%

Development of Stable Lyophilized Protein Drug Products

Remmele¹,

Krishnan²,

Callahan³

2012

CPB

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Freeze drying, or lyophilization is widely used for biopharmaceuticals to improve the long term storage stability of labile molecules. This review examines general theory and practice of rational lyophilization of biopharmaceuticals. Formulation development involving the selection of appropriate excipients, their associated physical properties, and mechanism of action in achieving a stable drug product are primary considerations for a successful lyophilization program. There are several parameters considered critical on the basis of their relationship to lyophilization cycle development and protein product stability. This along with the importance of analytical methods to provide insight toward understanding properties of drug product stability and cake structure are discussed. Also, aspects of instability found in lyophilized biopharmaceutical products, their degradation pathways and control are elucidated. Finally, container-closure requirements and drug product handling are described in context of the caveats to avoid compromising drug product quality.

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“…PGE2 is an inflammatory mediator that facilitates nociceptive signals. A significant increase in the level of PgE2 was found following intradermal injection of sodium dodecyl sulphate (99). Similarly, whereas histamine plays a key role in the immediate phase of inflammation that is elicited by topical 5-amino laevulinic acid photodynamic therapy (100), PgE2 and No are released during the prolonged erythema phase (101).…”

Section: Inflammatory Painmentioning

confidence: 97%

Microdialysis of Inflammatory Mediators in the Skin: A Review

Hersini¹,

Melgaard²,

Gazerani³

et al. 2014

Acta Derm Venerol

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Skin microdialysis is an established method for in vivo sample collection from the extracellular fluid space. This method has been extensively used in studies of inflammatory reactions in the skin of animals and humans. Skin microdialysis consists of the implantation of semi-permeable probes into the upper dermis, perfusion with a physiological buffer, and the recovery of the substances that diffused from the skin into the perfusion fluid. Microdialysis allows the simultaneous assessment of the temporal variations of inflammatory mediator release in the skin as well as the monitoring of vascular and sensory functions. By the aid of this technique, potential associations can be found between functional changes and a variety of substances and mediators released at the site of interest. This allows further insights into the possible mechanisms underlying physiological and pathophysiological events in the skin, including cutaneous inflammation. This review provides a comprehensive but not exhaustive review of the use of microdialysis in studies of experimental and clinical inflammatory reactions in the skin in animals and humans.

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Controlled dermal cell damage as human in vivo model for localised pain and inflammation

Cited by 10 publications

References 60 publications

A novel model of inflammatory pain in human skin involving topical application of sodium lauryl sulfate

A novel model of inflammatory pain in human skin involving topical application of sodium lauryl sulfate

Development of Stable Lyophilized Protein Drug Products

Microdialysis of Inflammatory Mediators in the Skin: A Review

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