Controlled Detachment of Chemically Glued Cells

Koo, Heebeom; Hahn, Sei Kwang; Yun, Seok Hyun

doi:10.1021/acs.bioconjchem.6b00546

Cited by 18 publications

(21 citation statements)

References 24 publications

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“…NAC produces similar effects as reduced glutathione on cell index and phalloïdin labeling (results not shown). Besides, glutathione is also reported to trigger the degradation of disulphide bonds (Koo et al 2016). Disulfide bonds formed by the oxidation of two cysteine residues to give cystine are essential for the stability of secreted and plasma-membrane proteins (reviewed in Trivedi et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Reduced glutathione decreases cell adhesion and increases cell volume

Géloën

Berger

2021

Preprint

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Glutathione is the most abundant thiol in animal cells. Reduced glutathione (GSH) is a major intracellular antioxidant neutralizing free radicals and detoxifying electrophiles. It plays important roles in many cellular processes, including cell differentiation, proliferation, and apoptosis. In the present study we demonstrate that extracellular concentration of reduced glutathione markedly increases cell volume within few hours, in a dose-response manner. Pre-incubation of cells with BSO, the inhibitor of 7-glutamylcysteine synthetase, responsible for the first step in intracellular glutathione synthesis did not change the effect of reduced glutathione on cell volume suggesting a mechanism limited to the interaction of extracellular reduced glutathione on cell membrane. Results show that reduced GSH decreases cell adhesion resulting in an increased cell volume. Since many cell types are able to transport of GSH out, the present results suggest that this could be a fundamental self-regulation of cell volume, giving the cells a self-control on their adhesion proteins.

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Section: Resultsmentioning

confidence: 99%

Reduced glutathione decreases cell adhesion and increases cell volume

Géloën

Berger

2021

Preprint

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“…A recent study by the same group has demonstrated that adhered cells could be detached by chemical stimuli. 74 They rst introduced an azido group onto Jurkat and A549 cells via metabolic glycoengineering using Ac 4 ManNAz. Later, azide-modied A549 and Jurkat cells reacted with DBCO-disulde-Tz and DBCO-disulde-TCO, respectively.…”

Section: Cell Adhesionmentioning

confidence: 99%

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

2019

Self Cite

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“…The usage of smart biodegradable cell linkages with on‐demand dynamic anchoring of cells may help overcoming some of those concerns. With this rationale, a biocompatible and chemically detachable cell glue system based on biorthogonal linkers connecting glycoengineered cells was successfully developed . Azide‐containing cells were functionalized with bioreducible tetrazine and trans‐cyclooctene linkers containing internal disulfide bonds, achieving fast cell–cell gluing upon mixing the two different cell groups.…”

Section: Cell‐rich Assembliesmentioning

confidence: 99%

Section: Cell‐rich Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Controlled Detachment of Chemically Glued Cells

Cited by 18 publications

References 24 publications

Reduced glutathione decreases cell adhesion and increases cell volume

Reduced glutathione decreases cell adhesion and increases cell volume

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

Advanced Bottom‐Up Engineering of Living Architectures

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