2000
DOI: 10.1021/es9908319
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Controlled Field Release of a Bioluminescent Genetically Engineered Microorganism for Bioremediation Process Monitoring and Control

Abstract: Pseudomonas fluorescens HK44 represents the first genetically engineered microorganism approved for field testing in the United States for bioremediation purposes. Strain HK44 harbors an introduced lux gene fused within a naphthalene degradative pathway, thereby allowing this recombinant microbe to bioluminesce as it degrades specific polyaromatic hydrocarbons such as naphthalene. The bioremediation process can therefore be monitored by the detection of light. P. fluorescens HK44 was inoculated into the vadose… Show more

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Cited by 165 publications
(73 citation statements)
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“…The introduced genetic circuits can vary greatly in complexity, however, and examples comprising natural sensor proteins [5,50] can be distinguished from those that involve "non-natural" sensor proteins with novel specificities, generated by directed evolution [51] or computational design [52]. Though involving few components, the approaches involving "non-natural" sensor proteins as well as the recent integration of a synthetic riboswitch capable of controlling migration of green fluorescent protein expressing bacteria towards the pesticide atrazine (followed by its degradation) [53] (see Fig.…”
Section: (I) Environmental (Whole-cell) Biosensorsmentioning
confidence: 99%
“…The introduced genetic circuits can vary greatly in complexity, however, and examples comprising natural sensor proteins [5,50] can be distinguished from those that involve "non-natural" sensor proteins with novel specificities, generated by directed evolution [51] or computational design [52]. Though involving few components, the approaches involving "non-natural" sensor proteins as well as the recent integration of a synthetic riboswitch capable of controlling migration of green fluorescent protein expressing bacteria towards the pesticide atrazine (followed by its degradation) [53] (see Fig.…”
Section: (I) Environmental (Whole-cell) Biosensorsmentioning
confidence: 99%
“…These "realistic" mesocosms would provide a suitable environment for testing synthetic microbial systems in a controlled setting. BER has supported pioneering the use of designed organisms to enhance process understanding at the field scale (Ripp et al 2000). Recent advances in genetic containment and control strategy greatly December 2010…”
Section: Deploy Synthetic (Or Nonsynthetic) Biology To Understand Andmentioning
confidence: 99%
“…The first United States Environmental Protection Agency (EPA) approved application of a genetically engineered microorganism for bioremediation purposes occurred in 1996 at an intermediate scale (4 m deep × 2.5 m diameter), semi-contained, and replicated soil lysimeter site at the Oak Ridge National Laboratory, Oak Ridge, Tennessee [1]. This organism, Pseudomonas fluorescens HK44, was capable of degrading polycyclic aromatic hydrocarbons (PAHs) and contained the genes for bioluminescence (luxCDABE) downstream of the salicylate inducible nahR promoter on a plasmid (pUTK21) construct [2].…”
Section: Introductionmentioning
confidence: 99%
“…Strain HK44 was sprayed on to layered 10 cm soil lifts 1 m deep artificially contaminated with a PAH mixture of naphthalene, anthracene, and phenanthrene [4]. Bioluminescence from these soil-borne bioreporters was then monitored using fiber optic and photomultiplier tube (PMT) interfaces over the ensuing two years to establish the potential for bioreporters to serve as bioremediation process monitoring and control tools [1]. Ancillary to this was the critical question of whether genetically engineered microorganisms like HK44 could even survive under conventional environmental pressures for this length of time due to the extra metabolic burdens and lack of fitness that these recombinant microorganisms perceivably bear.…”
Section: Introductionmentioning
confidence: 99%
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