Controlled FRET efficiency in nano-bio hybrid materials made from semiconductor quantum dots and bacteriorhodopsin

Bouchonville, Nicolas; Cigne, Anthony Le; Sukhanova, Alyona; Saab, Marie-Belle; Troyon, M.; Molinari, Michaël; Nabiev, Igor

doi:10.1117/12.930747

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…They demonstrated that accurate control of distance between QD and bR and reasonable overlap between QD emission and bR absorption spectrum result in complete control on FRET efficiency. They showed that electrostatic self-assembled QDstreptavidin on PM-biotin has the highest FRET efficiency [84]. These experiments declare that FRET is the main mechanism of QD quenching in bR-QD hybrids.…”

Section: Accepted Manuscriptmentioning

confidence: 96%

Bio-nano hybrid materials based on bacteriorhodopsin: Potential applications and future strategies

Mahyad

Janfaza

Hosseini

2015

Advances in Colloid and Interface Science

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Section: Accepted Manuscriptmentioning

confidence: 96%

Bio-nano hybrid materials based on bacteriorhodopsin: Potential applications and future strategies

Mahyad

Janfaza

Hosseini

2015

Advances in Colloid and Interface Science

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“…Finally, Bouchonville et al performed a detailed analysis of the effect of QD‐bR separation distance on the FRET efficiency (FRET E ) of the conjugate system. [ 22 ] While these studies demonstrate the ability of QD‐rhodopsin systems to engage in efficient FRET, no study to date has demonstrated the ability to interface a QD with rhodopsin‐expressing living cells for the realtime activation of the channel protein via FRET.…”

Section: Introductionmentioning

confidence: 99%

In Situ Self‐Assembly of Quantum Dots at the Plasma Membrane Mediates Energy Transfer‐Based Activation of Channelrhodopsin

Nag¹,

Muroski²,

Field

et al. 2021

Part & Part Syst Charact

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The use of nanoparticle (NP) bioconjugates to control the activity of membrane ion channels has recently emerged as a new paradigm for the activation of electrically excitable cells. An NP‐based strategy is reported for the specific activation of channelrhodopsin C1V1 (ChR‐C1V1) expressed in the plasma membrane of HEK 293T/17 cells. Hydrophilic CdSe/ZnS core–shell semiconductor quantum dots (QDs) are self‐assembled to the exofacial face of recombinantly expressed ChR‐C1V1 by metal affinity‐driven interaction of the QD ZnS shell with an N‐terminal hexahistidine tag displayed on ChR‐C1V1. This configuration enables the Förster resonance energy transfer (FRET)‐based excitation and activation of the 11‐cis‐retinal moiety of ChR‐C1V1 using the QD as a light harvesting transducer/energy donor. It is shown that the specific laser‐induced opening of the ChR‐C1V1 channel wherein the photoexcited QD (405 nm excitation, 530 nm emission) iteratively activates ChR‐C1V1 channels as confirmed using the voltage‐sensitive dye (VSD) bis‐(1,3‐diethylthiobarbituric acid)trimethine oxonol (DiSBAC2(3)). In the absence of the QD transducer, excitation of ChR‐C1V1‐expressing cells at 405 nm results in no activation of ChR‐C1V1. The results demonstrate the ability to controllably interface QDs with living cells for the activation of ChR membrane proteins and detail a new NP‐bioconjugate hybrid system for the specific activation of ion channels.

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“…Semiconductor quantum dots (QDs) is fluorescent nanocrystals promising in the field of photovoltaics, optoelectronics and biosensing [2], which have high one-photon and two-photon absorption cross-sections in a UV-and NIR spectral regions, respectively, can significantly improve the light sensitivity of BR by means of Förster resonance energy transfer (FRET) from QD to BR [3][4][5][6][7][8][9][10][11]. In turn, the high work efficiency of the QD-BR nano-bio hybrid material implies a large number of FRET elementary actions from QD to BR per time unit, which is in strong correlation with the QDs' excited state population.…”

Section: Physbiosymp17mentioning

confidence: 99%

Laser Irradiation as a Tool to Control the Resonance Energy Transfer in Bacteriorhodopsin–Quantum Dot Bio-Nano Hybrid Material

Krivenkov¹,

Samokhvalov²,

Chistyakov³

et al. 2018

KEn

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Bacteriorhodopsin (BR) is a natural photosensitive protein which can be considered promising in photovoltaics and optoelectronics because of its ability to produce a pronounced electrochemical response and controllably change its absorption spectrum under light excitation. However, its applicability is limited by its narrow absorption spectrum and low values of the absorption cross sections. Semiconductor quantum dots (QDs), which have high one-and two-photon absorption cross-sections in a UVand NIR spectral regions, respectively, can significantly improve the light sensitivity of BR by means of Förster resonance energy transfer (FRET) from QD to BR. In this work, we demonstrate the possibility to control the efficiency of FRET from QD to BR within electrostatically bound complexes of QD and purple membranes (PM) containing BR. We show that laser irradiation of QDs at different wavelengths leads to distinct changes (rise or decrease) of QD luminescence quantum yield (QY) without changing of QD structure. Such photo-induced changes in the QY of QD lead to a corresponding change in the efficiency of FRET. We have estimated efficiencies of FRET from QD to BR in the PM complexes composed of irradiated and non-irradiated QDs and found the increase in FRET efficiency with irradiated QDs.

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Controlled FRET efficiency in nano-bio hybrid materials made from semiconductor quantum dots and bacteriorhodopsin

Cited by 3 publications

References 33 publications

Bio-nano hybrid materials based on bacteriorhodopsin: Potential applications and future strategies

Bio-nano hybrid materials based on bacteriorhodopsin: Potential applications and future strategies

In Situ Self‐Assembly of Quantum Dots at the Plasma Membrane Mediates Energy Transfer‐Based Activation of Channelrhodopsin

Laser Irradiation as a Tool to Control the Resonance Energy Transfer in Bacteriorhodopsin–Quantum Dot Bio-Nano Hybrid Material

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