Controlled Functionalization of Water-Soluble Semiconductor Quantum Dots for Bioconjugation

Ranishenka, Bahdan; Ulashchik, Egor; Kruhlik, A. S.; Tatulchenkov, Maksim; Radchanka, Aliaksandra; Shmanai, Vadim V.; Artemyev, Mikhail

doi:10.1007/s10812-020-00941-3

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…The mixture was mixed well and incubated overnight at 7°C. Bovine serum albumin was used to demonstrate the efficiency of the modified QDs in the conjugation process ( Ranishenka et al, 2020 ).…”

Section: Functionalization Methodsmentioning

confidence: 99%

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Mirica

Stan²,

Chelcea³

et al. 2022

Front. Bioeng. Biotechnol.

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LFIA is one of the most successful analytical methods for various target molecules detection. As a recent example, LFIA tests have played an important role in mitigating the effects of the global pandemic with SARS-COV-2, due to their ability to rapidly detect infected individuals and stop further spreading of the virus. For this reason, researchers around the world have done tremendous efforts to improve their sensibility and specificity. The development of LFIA has many sensitive steps, but some of the most important ones are choosing the proper labeling probes, the functionalization method and the conjugation process. There are a series of labeling probes described in the specialized literature, such as gold nanoparticles (GNP), latex particles (LP), magnetic nanoparticles (MNP), quantum dots (QDs) and more recently carbon, silica and europium nanoparticles. The current review aims to present some of the most recent and promising methods for the functionalization of the labeling probes and the conjugation with biomolecules, such as antibodies and antigens. The last chapter is dedicated to a selection of conjugation protocols, applicable to various types of nanoparticles (GNPs, QDs, magnetic nanoparticles, carbon nanoparticles, silica and europium nanoparticles).

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Section: Functionalization Methodsmentioning

confidence: 99%

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Mirica

Stan²,

Chelcea³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Carbodiimide-mediated coupling chemistry is a well-established method for covalently tethering QDs to secondary species such as carbon nanotubes, metallic nanoparticles, proteins, small molecules, antibodies, and more recently other QDs to form heterostructures. ,− Carbodiimide-mediated coupling between terminal functional groups of QD-adsorbed ligands selectively yields heterostructures and affords control over the rate and extent of reaction, which are distinct advantages in comparison to the formation of heterostructures via bifunctional linkers . Our group’s recent efforts have established the validity of utilizing carbodiimide-mediated coupling chemistry to selectively tether QDs through formation of an amide bond between terminal functional groups of respective capping ligands.…”

Section: Introductionmentioning

confidence: 99%

Excited-State Charge Transfer and Extended Charge Separation within Covalently Tethered Type-II CdSe/CdTe Quantum Dot Heterostructures: Colloidal and Multilayered Systems

McGranahan

Wolfe

Falca

et al. 2021

ACS Appl. Mater. Interfaces

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We used N,N′-dicyclohexylcarbodiimide (DCC) coupling chemistry to synthesize (1) heterostructures of CdSe and CdTe quantum dots (QDs) in colloidal dispersions and (2) heterostructures of CdSe and CdTe QDs, as well as CdS and CdSe QDs, immobilized on metal oxide thin films. The DCC-mediated formation of amide bonds between terminal carboxylic acid and amine groups of ligands on different QDs drove the formation of heterostructures. This cross-linking mechanism selectively yields heterostructures and prohibits the undesired formation of homostructures consisting of just one type of QD. Products of adsorption, ligand-exchange, and covalent-coupling reactions were characterized by transmission electron microscopy and ATR-FTIR, 1 H NMR, electronic absorption, steady-state emission, and timeresolved emission spectroscopy. Ground-state absorption spectra of constituent QDs were unperturbed upon incorporation into heterostructures, enabling control over electronic properties. Heterostructures of CdSe and CdTe QDs exhibit type-II interfacial energetic offsets that promote charge separation following excitation of either QD. Indeed, photoexcited CdTe QDs transferred electrons to CdSe, and photoexcited CdSe QDs transferred holes to CdTe, on time scales of 10−100 ns, as evidenced by dynamic quenching of band-edge and trap-state emission. Mixed dispersions of noninteracting QDs did not undergo excited-state charge transfer. Constructing heterostructures on TiO 2 thin films introduced an additional charge-transfer pathway, electron transfer from QDs to TiO 2 , which occurred on subnanosecond time scales and enabled extended spatial separation of photogenerated electrons and holes. Our results reveal that carbodiimide coupling chemistry can be used to tether colloidal QDs selectively and covalently to each other, yielding dispersed or immobilized heterostructures with programmable compositions and energetic offsets that can undergo efficient excited-state interfacial electron transfer.

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Emergence of Quantum Dots as Innovative Tools for Early Diagnosis and Advanced Treatment of Breast Cancer

Díaz‐García,

Díaz‐Sánchez,

Álvarez‐Conde

et al. 2024

ChemMedChem

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Quantum dots (QDs) semiconducting nanomaterials, have garnered attention due to their distinctive properties, including small size, high luminescence, and biocompatibility. In the context of triple‐negative breast cancer (TNBC), notorious for its resistance to conventional treatments, QDs exhibit promising potential for enhancing diagnostic imaging and providing targeted therapies. This review underscores recent advancements in the utilization of QDs in imaging techniques, such as fluorescence tomography and magnetic resonance imaging, aiming at the early and precise detection of tumors. Emphasis is placed on the significance of QD design, synthesis and functionalization processes as well as their use in innovative strategies for targeted drug delivery, capitalizing on their ability to selectively deliver therapeutic agents to cancer cells. As the research in this field advances rapidly, this review covers a classification of QDs according to their composition, the characterization techniques than can be used to determine their properties and, subsequently, emphasizes recent findings in the field of TNBC‐targeting, highlighting the imperative need to address challenges, like potential toxicity or methodologies standardization. Collectively, the findings explored thus far suggest that QDs could pave the way for early diagnosis and effective therapy of TNBC, representing a significant stride toward precise and personalized strategies in treating TNBC.

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Controlled Functionalization of Water-Soluble Semiconductor Quantum Dots for Bioconjugation

Cited by 6 publications

References 13 publications

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Excited-State Charge Transfer and Extended Charge Separation within Covalently Tethered Type-II CdSe/CdTe Quantum Dot Heterostructures: Colloidal and Multilayered Systems

Emergence of Quantum Dots as Innovative Tools for Early Diagnosis and Advanced Treatment of Breast Cancer

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