Controlled protein adsorption on PMOXA/PAA based coatings by thermally induced immobilization

Mumtaz, Fatima; Chen, Chao-Shi; Zhu, Haikun; Pan, Chao; Wang, Yanmei

doi:10.1016/j.apsusc.2018.01.010

Cited by 26 publications

(11 citation statements)

References 45 publications

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“…The surface hydrophilicity/hydrophobicity is closely related to the protein adsorption and biocompatibility. Generally speaking, the good wettability is helpful to prevent the nonspecific protein adhesion and promote the cell adhesion and proliferation [ 32 ]. As can be seen from Figure 3(b) , the water contact angle of the blank titanium decreased obviously after anodizing; it was considered that the introduction of a large amount of oxygen elements and the special nanoporous structure can contribute to the excellent hydrophilicity.…”

Section: Resultsmentioning

confidence: 99%

Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities

Lin

Zhang

Yang

et al. 2021

Stem Cells International

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Titanium and its alloys are widely used in blood-contacting implantable and interventional medical devices; however, their biocompatibility is still facing great challenges. In the present study, in order to improve the biocompatibility and antibacterial activities of titanium, TiO2 nanotubes were firstly in situ prepared on the titanium surface by anodization, followed by the introduction of polyacrylic acid (PAA) and gentamicin (GS) on the nanotube surface by layer-by-layer assembly, and finally, zinc ions were loaded on the surface to further improve the bioactivities. The nanotubes displayed excellent hydrophilicity and special nanotube-like structure, which can selectively promote the albumin adsorption, enhance the blood compatibility, and promote the growth of endothelial cells to some degree. After the introduction of PAA and GS, although the superhydrophilicity cannot be achieved, the results of platelet adhesion, cyclic guanosine monophosphate (cGMP) activity, hemolysis rate, and activated partial thromboplastin time (APTT) showed that the blood compatibility was improved, and the blood compatibility was further enhanced after zinc ion loading. On the other hand, the modified surface showed good cytocompatibility to endothelial cells. The introduction of PAA and zinc ions not only promoted the adhesion and proliferation of endothelial cells but also upregulated expression of vascular endothelial growth factor (VEGF) and nitric oxide (NO). The slow and continuous release of GS and Zn2+ over 14 days can significantly improve the antibacterial properties. Therefore, the present study provides an effective method for the surface modification of titanium-based blood-contacting materials to simultaneously endow with good blood compatibility, endothelial growth behaviors, and antibacterial properties.

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Section: Resultsmentioning

confidence: 99%

Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities

Lin

Zhang

Yang

et al. 2021

Stem Cells International

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Section: Antibacterial Activitiesmentioning

confidence: 97%

Assembly of Gentamicin and Zn2+ Loaded Coatings on TiO2 Nanotubes to Synergistically Improve the Blood Compatibility, Endothelial Cell Growth and Antibacterial Activities

Pan

Yang

et al. 2020

Preprint

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Titanium and its alloys are widely used in blood contacting implantable and interventional medical devices; however, their biocompatibility is still facing great challenges. In this study, with the aim of improving the biocompatibility and antibacterial activities of titanium, TiO2 nanotubes with a diameter of about 30 nm were firstly prepared on the titanium surface by anodization, followed by the introduction of polyacrylic acid (PAA) and gentamicin (GS) on the nanotube surface by layer-by-layer method, and finally zinc ions were loaded into the surface to improve the bioactivities. The nanotubes have excellent hydrophilic properties and special nanotube-like structure, which can selectively promote the albumin adsorption and enhance the blood compatibility and promote the growth and functional expression of endothelial cells to a certain extent. After the introduction of PAA and GS, although the super-hydrophilicity cannot be achieved, the results of platelet adhesion, cGMP activity, hemolysis rate and partial thromboplastin time (APTT) showed that the blood compatibility was improved, and the blood compatibility was further enhanced after zinc ions loading on the surface. On the other hand, the surface modified materials showed good cytocompatibility to endothelial cells. The introduction of PAA and zinc ions not only promoted the adhesion and proliferation of endothelial cells, but also up-regulated expression of vascular endothelial growth factor (VEGF) and nitric oxide (NO). The slow and continuous release of GS and Zn2+ for more than 14 days, which can significantly improve the antibacterial properties of the materials. Therefore, the present study provides an effective method for the surface modification of titanium-based blood-contacting materials to simultaneously endow with good blood compatibility, endothelial growth behaviors and antibacterial properties.

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“…hydrophilicity and biocompatibility) to PEG and less degradation than PEG. [25][26][27][28][29] Cavallaro and coworkers reported that the films developed via plasma deposition of 2-methyl-2-oxazoline (MOXA) could resist the adsorption of Staphylococcus epidermidis and cells by more than 90% regardless of the type of substrate used. 30 Bai and coworkers developed poly(2-methyl-2-oxazoline-random-glycidyl methacrylate) (PMOXA-r-GMA) comb copolymers.…”

Section: Introductionmentioning

confidence: 99%

“…[38][39][40][41][42][43] Inspired by the stability and low fouling ability of PMOXA based coatings and swelling/deswelling behaviors of PNIPAM chains with temperature, herein our purpose is to create binary mixed polymer brushes, on which the adsorption of protein could be adjusted through the change of the hydrophobic/ hydrophilic behavior of mixed polymer brushes with temperature in an aqueous environment. Firstly, the comb copolymer, PMOXA-r-GMA was synthesized via cationic ring opening polymerization (CROP) of MOXA followed by its random copolymerization with GMA according to our previous work; 28,31,44 and poly(glycidyl methacrylate)-block-poly(N-isopropyl acrylamide)block-polystyrene (PGMA-b-PNIPAM-b-PSt) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of GMA, N-isopropyl acrylamide (NIPAM), and styrene (St) step by step. Then the mixed brushes based on PMOXA and PNIPAM-b-PSt were fabricated by spin coating the mixture of PMOXA-r-GMA and PGMA-b-PNIPAM-b-PSt solutions onto silicon or glass substrates followed by an annealing protocol.…”

Section: Introductionmentioning

confidence: 99%

A binary mixed polymer brush coating with adjusted hydrophobic property to control protein adsorption

Chen

Atif

et al. 2021

Mater. Adv.

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Controlled protein adsorption on PMOXA/PAA based coatings by thermally induced immobilization

Cited by 26 publications

References 45 publications

Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities

Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities

Assembly of Gentamicin and Zn2+ Loaded Coatings on TiO2 Nanotubes to Synergistically Improve the Blood Compatibility, Endothelial Cell Growth and Antibacterial Activities

A binary mixed polymer brush coating with adjusted hydrophobic property to control protein adsorption

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