Controlled release of BMP-2 from alginate nanohydrogels enhanced osteogenic differentiation of human bone marrow stromal cells

Abstract

A new controlled release system is proposed for bone morphogenetic proteins (BMP)-induced osteoblastic growth and differentiation of the human bone marrow stromal cells (hBMSCs) using injectable nanohydrogels. Alginate nanohydrogels (ANH) containing BMP-2 (BMP-2@ANH) were prepared using a reverse emulsificationdiffusion technique. The mean particle diameter of the BMP-2@ANH measured by dynamic light scattering was 520 nm. The proliferation of hBMSCs was promoted with the BMP-2@ANH. The controlled release of BMP-2 from the BMP-2@ANH enhanced the differentiation of hBMSCs into osteoblasts, which was confirmed by RT-PCR and alkaline phosphate activity. With the BMP-2@ANH, calcification and phosphate deposits on the cells were confirmed by alizarin red-S staining and von-kossa staining, respectively, showing that the differentiation of hBMSCs into osteoblasts was activated by the BMP-2 released from the BMP-2@ANH. Growth factor delivery using a nanohydrogel is a potential method for facilitating the differentiation of stem cells in vivo.

Introduction

“…Alginate (ALG)-based hydrogels, covalently modified with RDG-containing peptides, were proposed for bone regeneration, with particular attention to the critical aspects related to scaffold degradation [3,4,128]. ALG hydrogels were also studied in the form of nanohydrogels by Lim et al [75] in order to enhance osteogenic differentiation of human bone marrow stromal cells by the controlled release of a growth factor.…”

Efficient Absorption of Antibiotic from Aqueous Solutions over MnO2@SA/Mn Beads and Their In Situ Regeneration by Heterogeneous Fenton-Like Reaction

“…Sodium alginate (SA), a natural polysaccharide extracted from seaweed and comprised homopolymeric blocks of guluronate blocks and mannuronate blocks, was used extensively as supported adsorbent owing to its advantages of biocompatibility, low toxic properties and budget, and ease of gelation [10,11]. Several alginate-based adsorbents including alginate nanohybrids [12], alginate nanohydrogel [13], alginate fiber [14], alginate bead [15], and alginate film [16] have already been explored for organic pollution adsorption applications. In contrast, the alginate beads have been more widely used than the fiber, nanoparticles, or film forms because of their simple fabrication and recovery process, controllable particle dimension, and excellent dispersion stability [17].…”

Dual growth factor-loaded core-shell polymer microcapsules can promote osteogenesis and angiogenesis

“…5 Consequently, many carrier systems for the localized and controlled delivery of a single GF have been explored and expanded further to the use of multiple GFs. [6][7][8][9] Several groups have proven the importance of dual GF delivery in the regeneration of complex tissues. 5,[10][11][12][13] In particular, the temporally controlled delivery of dual GFs for stem cells differentiation has been extensively investigated.…”