Controlled release of hydrogen sulfide significantly reduces ROS stress and increases dopamine levels in transgenic <i>C. elegans</i>

Ali, Rafat; Pal, Hilal Ahmad; Hameed, Rohil; Nazir, Aamir; Verma, Sandeep

doi:10.1039/c9cc05153h

Cited by 17 publications

(16 citation statements)

References 45 publications

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“…A H 2 S-sensing naphthalimide-based peptide conjugate (HSNPc) was synthesized by both, solution phase [36][37][38] and solid-phase peptide synthesis using standard protocols. 39 4-Bromo naphthalic anhydride was used as a precursor molecule. Conjugation of alanine to this anhydride followed by substitution of bromine by dinitrobenzene probe at the fourth position of 4-bromo naphthalic anhydride carried out in solution phase.…”

Section: Resultsmentioning

confidence: 99%

A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

et al. 2021

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Section: Resultsmentioning

confidence: 99%

A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

et al. 2021

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“…No. D399) as described previously [21]. Brie y, stock solution (10 mM) was prepared fresh in absolute alcohol, which was diluted (100 µM) in phosphate buffer saline (for 1 X PBS Na 2 HPO 4 1.44 g, KH 2 PO 4 240 mg, NaCl 8 g, and KCl 200 mg).…”

Section: Quanti Cation Of Reactive Oxygen Speciesmentioning

confidence: 99%

Targeted Downregulation of Estradiol Binding Na+/H+ Exchanger Nhx-2, Mimics Calorie Restriction, Extends Reproductive Longevity and Ameliorates Effects Associated With Alpha Synuclein Aggregation In C. Elegans

Shukla

Kumar²,

Sarkar³

et al. 2021

Preprint

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Setting in of reproductive senescence (RS) gives rise to several changes, making aged individuals susceptible to multiple disorders including neurodegenerative diseases, cardiovascular ailments and bone disorders amongst others. The present study, employing transgenic C. elegans that expresses ‘human’ alpha synuclein, endeavors to decipher the association of reproductive senescence with age-associated neurodegenerative diseases and behavioral ageing, under normal conditions and after being probed with estradiol. We carried out RNAi induced silencing of a subset of 22 genes that are known to delay RS, followed by studies on alpha-Synuclein aggregation and associated effects. These studies led us to functional characterization of the Na+/H+ exchanger; nhx-2, expressed exclusively in gut. We found that RNAi of nhx-2 not only ameliorates the effects associated with alpha-Synuclein aggregation, but it also attunes effects related to behavioral aging including that of reproductive health-span and neuroprotection via mimicking dietary restriction, as it alters food absorption from the gut. We further elucidated that these effects are Sir-2.1 driven as nhx-2 knock out did not delay reproductive senescence in knock down condition of sir-2.1. To substantiate our findings, we performed whole transcriptome analysis in nhx-2 mutant strain. Our data revealed differential expression of 61 out of 62 hallmark genes of CR described by GenDR, in knock out condition of nhx-2. As estradiol plays a central role in both reproductive health as well as neuronal health, we subjected worms to exogenous estradiol treatment and observed that it led to elevated levels of nhx-2. Studies on structural binding analysis demonstrated significant binding potential of estradiol receptor NHR-14 with nhx-2 gene and ChIP analysis revealed that estradiol treatment gives rise to enhanced NHX-2 levels through inducing the promoter specific histone H3 acetylation (H3K9) and lysine methylation (H3K4me3). These studies identify nhx-2 as an important modulator that extends reproductive longevity and ameliorates effects associated with alpha synuclein aggregation in C elegans.

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“…[36,37] Recent studies have shown that H 2 S plays a role in cell metabolism and redox balance. [38][39][40] ROS are ubiquitous in human organs and tis sues and are mainly produced by external stimulation or as byproducts of metabolic processes. [41,42] However, excessive ROS in cells causes oxidative stress, which may lead to oxida tive stressinduced diseases, including acute hypoxiaischemia/ reperfusion, degenerative diseases, epilepsy, and cardiovascular diseases.…”

Section: H 2 S-mediated Alleviation Of Ros In Mammalian Cell Communitiesmentioning

confidence: 99%

Photothermally Activated Coacervate Model Protocells as Signal Transducers Endow Mammalian Cells with Light Sensitivity

Yao

Zhang

et al. 2021

Advanced Biology

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The development of a novel photothermally activated coacervate model protocell is reported as a signal transducer to endow mammalian cells with light sensitivity. In this system, near‐infrared light irradiation triggers H2S release in coacervate model protocells, leading to modulation of the behavior of living cells. The functional coacervate model protocells are prepared by loading metal‐alloyed plasmonic nanoparticles and an H2S donor into the liquid coacervate microdroplets. Upon light irradiation, the H2S signal messenger is released through the photothermal effect of plasmonic nanoparticles and photothermal mediated pyrolysis of the H2S donor. The H2S signal is delivered to the mammalian cell community to trigger depletion of reactive oxygen species, reduce the activity of lactate dehydrogenase and improve cell viability. This study provides a new approach to the implementation of chemical signaling in artificial cell colonies and protocell/living cell consortia. The photothermal protocell system offers a powerful platform for light modulation of the behavior of mammalian cells and shows great promise for biomedical applications.

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Controlled release of hydrogen sulfide significantly reduces ROS stress and increases dopamine levels in transgenic C. elegans

Abstract: A novel peptide based system has been developed that exhibits slow and sustained H2S release thereby reducing hydrogen peroxide-induced oxidative stress and increasing dopamine levels in a transgenic C. elegans model.

Cited by 17 publications

References 45 publications

A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

Targeted Downregulation of Estradiol Binding Na+/H+ Exchanger Nhx-2, Mimics Calorie Restriction, Extends Reproductive Longevity and Ameliorates Effects Associated With Alpha Synuclein Aggregation In C. Elegans

Photothermally Activated Coacervate Model Protocells as Signal Transducers Endow Mammalian Cells with Light Sensitivity

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