Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration

Adeli-Sardou, Mahboubeh; Yaghoobi, Mohammad Mehdi; Torkzadeh‐Mahani, Masoud; Dodel, Masoumeh

doi:10.1016/j.ijbiomac.2018.11.237

Cited by 145 publications

(73 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[ 13 ] The scaffold with a large ratio of surface area to volume will have higher opportunities for the cells to attach and migrate. [ 14–16 ] Moreover, different types of scaffolds in tissue engineering are used to create microenvironment to mimic the natural extracellular matrix (ECM), which supports cell growth and new tissue formation. [ 17,18 ] Therefore, current efforts in skin tissue engineering focused on the fabrication of 3D scaffolds to emulate ECM of natural skin.…”

Section: Introductionmentioning

confidence: 99%

“…Several researchers have investigated different methods to fabricate biomimetic skin scaffold. [ 16,17,19,20 ] The first commonly used approach is the electrospinning technique that has been intensively applied due to the advantages of reproducibility, simplicity, and diversity in producing fibers with micro‐ or nanoscale diameters and different topographical features. [ 21,22 ] The fibers' membrane produced by electrospinning can provide large surface areas for cells to attach and proliferate, which could also enhance the penetration of nutrients and oxygen.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Liu

Zhang

et al. 2020

Macro Materials & Eng

View full text Add to dashboard Cite

Skin injuries are an urgent health issue, which raises a great concern in the clinic. Although numerous strategies have been proposed to fabricate skin substitutes for treatment of wounds over the past several decades, fabricating an ideal skin substitute to replace the damaged one can still be a problem. In this study, a novel biomimetic 3D composite skin scaffold is fabricated by combining electrohydrodynamic (EHD) jetting, electrospinning, and coating techniques. Here, the first polycaprolactone (PCL) porous structure is produced by the EHD jetting. Next, the second polylactic acid (PLA) membrane consisted of nanoscale fibers is prepared on the PCL porous structure via the electrospinning. The PCL porous structure and PLA fibers membrane can mimic the dermis and epidermis layer, respectively. Furthermore, gelatin is used as coating solution to enhance the biocompatibility of the scaffold. The structure and morphology of the fabricated scaffolds are analyzed, and the mechanical properties are investigated as well. Moreover, the in vitro and in vivo experiments demonstrate the biocompatibility of the materials and the fabrication process. In conclusion, these results demonstrate that the composite scaffold is effective and holds great potential for skin regeneration in the clinic.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Liu

Zhang

et al. 2020

Macro Materials & Eng

View full text Add to dashboard Cite

show abstract

“…Similarly as collagen, also gelatin is the most promising for skin tissue engineering and wound healing applications in combination with various synthetic and natural polymers. For example, gelatin was combined with polyurethane [109], PLA [11,17], and particularly with PCL, where it was incorporated into core-shell PCL/gelatin nanofibers as the core polymer [110] or electrospun independently of PCL using a double-nozzle technique, which resulted in creation of two types of nanofibers in the scaffolds, either mixed [111] or arranged in separate gelatin and PCL layers [112]. Gelatin was also combined with a copolymer of lactic acid and caprolactone P(LLA-CL) in the form of blends [113] or in the form of coaxial nanofibers with P(LLA-CL)/gelatin shell and albumin core containing epidermal growth factor, insulin, hydrocortisone, and retinoic acid [114].…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

View full text Add to dashboard Cite

Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

show abstract

“…The combination of various polymers, nanoparticles, and other components is a strategy commonly used to obtain hybrid materials possessing properties better than those of the individual constituents, regarding their use in scaffolds for tissue engineering or as material for wound dressing [21]. For example, synthetic polymers do not contain adhesion motifs recognizable by cell adhesion receptors, and combination with nature-derived polymers, which are proteins (collagen, gelatin, keratin, fibrin [6, 10, 22,23]) or polysaccharides (hyaluronic acid, sulfated glycosaminoglycans, such as heparin [24,25]) can endow them with these motifs, because these polymers are often components of ECM.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Bačáková¹,

Zikmundová²,

Pajorová³

et al. 2020

Applications of Nanobiotechnology

View full text Add to dashboard Cite

Nanofibrous scaffolds are popular materials in all areas of tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. In this chapter, we focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is an organ with several vitally important functions, particularly barrier, thermoregulatory, and sensory functions. Nanofibrous meshes not only serve as carriers for skin cells but also can prevent the penetration of microbes into wounds and can keep appropriate moisture in the damaged skin. The nanofibrous meshes have been prepared from a wide range of synthetic and nature-derived polymers. This review is concentrated on synthetic non-degradable and degradable polymers, which have been explored for skin tissue engineering and wound healing. These synthetic polymers were often combined with natural polymers of the protein or polysaccharide nature, which improved their attractiveness for cell colonization. The nanofibrous scaffolds can also be loaded with various bioactive molecules, such as growth factors, hormones, vitamins, antioxidants, antimicrobial, and antitumor agents. In advanced tissue engineering approaches, the cells on the nanofibrous scaffolds are cultured in dynamic bioreactors enabling appropriate mechanical stimulation of cells and at air-liquid interface. This chapter summarizes recent results achieved in the field of nanofiber-based skin tissue engineering, including results of our research group.

show abstract

Controlled release of lawsone from polycaprolactone/gelatin electrospun nano fibers for skin tissue regeneration

Cited by 145 publications

References 71 publications

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

Contact Info

Product

Resources

About