Controlled release of oxygen from PLGA-alginate layered matrix and its in vitro characterization on the viability of muscle cells under hypoxic environment

Abdi, Syed Izhar Haider; Choi, Ju Young; Lau, Hui Chong; Lim, Jeong Ok

doi:10.1007/s13770-013-0391-7

Cited by 40 publications

(33 citation statements)

References 20 publications

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“…Most of previously reported particulate oxygen‐releasing materials have the drawback of short release periods of 24–48 hr (Abdi, Choi, Lau, & Lim, ; Cook et al, ; Mallepally et al, ; Ng et al, ; Seekell et al, ; Ward et al, ), but a few studies reported more sustained oxygen release (Lee et al, ; Li et al, ; Montazeri et al, ). Hollow PCL microspheres encapsulating PFC emulsions could maintain oxygen tension above 10% for about 6 days, but these microspheres were approximately 300 μm in diameter, one order of magnitude larger than mammalian cells (Lee et al, ).…”

Section: Discussionmentioning

confidence: 99%

Oxygen‐releasing polycaprolactone/calcium peroxide composite microspheres

2019

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Oxygen‐releasing polycaprolactone/calcium peroxide (PCL/CaO2) composite microspheres were fabricated via homogenization, electrospray with a single nozzle, and electrospray with a co‐axial nozzle, resulting in homogenized, single‐walled, and double‐walled microspheres, respectively. Scanning electron microscopy revealed that homogenized microspheres had pores, while electrosprayed microspheres did not. Alizarin Red S staining showed a core‐shell structure for double‐walled microspheres. In a hypoxia incubator, single‐walled, double‐walled, and homogenized microspheres could maintain oxygen tension in PBS at or above 10% for approximately 5, 4, and 3 days, respectively. All the PCL/CaO2 microspheres could support viability of pancreatic β‐cell line MIN6 cells in 2D cultures in a hypoxia incubator for 1 week, with the cells supported by double‐walled and homogenized microspheres exhibiting the highest and the lowest metabolic activity, respectively. For 3D MIN6 cell cultures in a hypoxia incubator, single‐walled and homogenized PCL/CaO2 microspheres led to the highest and the lowest live cell densities, respectively. Double‐walled and single‐walled microspheres provided the best support for 2D and 3D cultures, respectively, suggesting that they are suitable for different applications.

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Section: Discussionmentioning

confidence: 99%

Oxygen‐releasing polycaprolactone/calcium peroxide composite microspheres

2019

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“…The lack of control over the organization of the vasculature hampers the function of the constructs. To overcome this limitation, recent studies have suggested the incorporation of cells or biofactors in the engineered tissues, which can accelerate the vascularization of the implanted construct and improve the long-term tissue survival [ 33 , 34 , 120 , 121 ]. The mixture of vascular cells in the structure forms randomly distributed capillary-like vessels that conduct blood flow very slowly with limited functional enhancement [ 38 ].…”

Section: Vascularization Of Cardiovascular Tissuesmentioning

confidence: 99%

3D Bioprinting and In Vitro Cardiovascular Tissue Modeling

Jang

2017

Bioengineering

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Numerous microfabrication approaches have been developed to recapitulate morphologically and functionally organized tissue microarchitectures in vitro; however, the technical and operational limitations remain to be overcome. 3D printing technology facilitates the building of a construct containing biomaterials and cells in desired organizations and shapes that have physiologically relevant geometry, complexity, and micro-environmental cues. The selection of biomaterials for 3D printing is considered one of the most critical factors to achieve tissue function. It has been reported that some printable biomaterials, having extracellular matrix-like intrinsic microenvironment factors, were capable of regulating stem cell fate and phenotype. In particular, this technology can control the spatial positions of cells, and provide topological, chemical, and complex cues, allowing neovascularization and maturation in the engineered cardiovascular tissues. This review will delineate the state-of-the-art 3D bioprinting techniques in the field of cardiovascular tissue engineering and their applications in translational medicine. In addition, this review will describe 3D printing-based pre-vascularization technologies correlated with implementing blood perfusion throughout the engineered tissue equivalent. The described engineering method may offer a unique approach that results in the physiological mimicry of human cardiovascular tissues to aid in drug development and therapeutic approaches.

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“…These oxygenated PMs can be considered intelligent biomaterials, 11 because oxygen delivery is triggered upon contact with water (H 2 O), and their application allows both the structural defects of tissue reconstruction and recovery of the tissue functions lost due to damage. 12,13 Some of these inorganic biomaterials are used as oxygen generators and releasers at the application site, such as sodium percarbonate, 14,15 magnesium peroxide, 16 hydrogen peroxide (H 2 O 2 ), 17,18 fluorinated compounds, 16 pyridine endoperoxides, 19 and calcium peroxide (CPO). 20 In 2015, Lee et al developed hollow microparticles (HPs) containing oxygen transporter as a structured system (scaffolds) that allowed the uniform distribution of oxygen to the adhered cells in the HPs.…”

Section: Introductionmentioning

confidence: 99%

“…17 Alginate coated poly(lactic-co-glycolic acid) (PLGA) microspheres with encapsulated H 2 O 2 and their application to muscle cells under hypoxia condition have also been demonstrated. 18 The dual layered system (alginate and PLGA) allowed the controlled release of the oxygen at the optimal level for the survival of the muscle cells. Several approaches have been employed to design and fabricate polymer particles, such as solvent evaporation, emulsion diffusion, nanoprecipitation, microfluidic device, and electrospray.…”

Section: Introductionmentioning

confidence: 99%

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Morais¹,

Wang²,

Vieira³

et al. 2020

IJN

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Background: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ =-16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.

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Controlled release of oxygen from PLGA-alginate layered matrix and its in vitro characterization on the viability of muscle cells under hypoxic environment

Cited by 40 publications

References 20 publications

Oxygen‐releasing polycaprolactone/calcium peroxide composite microspheres

Oxygen‐releasing polycaprolactone/calcium peroxide composite microspheres

3D Bioprinting and In Vitro Cardiovascular Tissue Modeling

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

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