Controlled release of plasmid DNA from hydrogels prepared from gelatin cationized by different amine compounds

Kubota, Toshihiro; Tomoshige, Ryuji; Iwanaga, Kazunori; Kakemi, Masawo; Tabata, Yasuhiko

doi:10.1016/j.jconrel.2006.02.003

Cited by 50 publications

(31 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[8,9] Briefly, a 1% (w/v) gelatin solution was prepared in 0.1 M phosphate buffer (pH 5.3), and for each mol of gelatin carboxyl groups, 3 mol of EDC and 50 mol of SPM were added. The final pH was adjusted to 5.0 and the solution allowed to react for 18 h in a warm bath at 37 8C.…”

Section: Synthesis Of Cgmentioning

confidence: 99%

“…[5,6] Among the polymers currently under investigation for non-viral gene delivery, cationized gelatin (CG) has demonstrated great potential in transfecting several types of cells in the form of hydrogels or complexes. [7][8][9][10] We were the first to attempt the use of CG to form nanoparticles using the ionic gelation technique; only recently, the use of these CG nanoparticles for transfecting the ocular epithelium was proposed. [11] Gelatin cationized with the low molecular polyamine spermine was the most promising among the studied cationized proteins in terms of safety, formation of nanoparticles with suitable physicochemical properties, We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hybrid Nanoparticle Design Based on Cationized Gelatin and the Polyanions Dextran Sulfate and Chondroitin Sulfate for Ocular Gene Therapy

Zorzi

Párraga

Seijo

et al. 2011

Macromolecular Bioscience

View full text Add to dashboard Cite

We describe the development of hybrid nanoparticles composed of cationized gelatin and the polyanions CS and DS for gene therapy in the ocular surface. The physicochemical properties of the nanoparticles that impact their bioperformance, such as average size and zeta potential, can be conveniently modulated by changing the ratio of polymers and the crosslinker. These systems associate plasmid DNA and are able to protect it from DNase I degradation. We corroborate that the introduction of CS or DS in the formulation decreases the in vitro toxicity of the nanoparticles to human corneal cells without compromising the transfection efficiency. These nanoparticles are potential candidates for the development of safer and more effective nanomedicines for ocular therapy.

show abstract

Section: Synthesis Of Cgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticle Design Based on Cationized Gelatin and the Polyanions Dextran Sulfate and Chondroitin Sulfate for Ocular Gene Therapy

Zorzi

Párraga

Seijo

et al. 2011

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…For example, synthetic polymers such as poly (lactic-co-glycolic acid) (PLGA) and natural polymers like gelatin and chitosan are being investigated. [17][18][19][20] Although these biocompatible and biodegradable materials have excellent characteristics and are indispensable as drug delivery systems, they suffer from several potential drawbacks including poor compatibility with certain drugs, To evaluate the usability of silk protein (sericin, SC) as a sustained-release material, the physicochemical properties of SC and the release profiles of model drugs from SC gel, sponge and film were studied. Heat aids the dissolution of SC.…”

Section: )mentioning

confidence: 99%

Use of Silk Protein, Sericin, as a Sustained-Release Material in the Form of a Gel, Sponge and Film

et al. 2010

View full text Add to dashboard Cite

Sericin (SC) is a component of silk protein in the silkworm cocoon. Silk protein consists of fibroin as the fiber and SC as the glue. SC comprises 25-30% of the silkworm (Bombyx mori) cocoon.1) Although SC is composed of 18 different amino acids, it contains a high number of polar side chains with hydroxyl, carboxyl and amino groups.1) Isolation and characterization of SC components from the cocoon of Bombyx mori showed that SC primarily consists of three polypeptides with molecular weights of 150, 250 and 400 kDa. The phenylthiocarbamyl (PTC) method 2) showed that the amino acid compositions of all three SC have high contents of serine (Ser, 33.2-39.0%), glycine (Gly, 14.1-16.0%) and aspartic acid/asparagine (Asp/Asn, 11.3-15.7%). The structural analysis and cloning of SC genes Ser1 and Ser2 (Src-2) have been described. [3][4][5][6] Correspondence of the amino acid composition of SC with these genes suggested that the 150-and 400-kDa SCs correspond to Ser1 proteins (77-331 kDa) encoded by the Ser1 gene, and that the 250-kDa SC corresponds to S-2 protein (227 kDa) encoded by the Src-2 gene.2) The most abundant component is the largest SC (400 kDa), which corresponds to the Ser1C protein (331 kDa).2) A repetitive 38-amino acid sequence rich in Ser (40%) dominates a large part of the Ser1C protein and is predicted to have a strong tendency to form b-sheet structure. Another part of the Ser1C protein is hydrophilic and has a high content of charged residues including acidic (glutamic acid (Glu) and Asp) and basic (lysine (Lys) and arginine (Arg)) amino acids. 6) In contrast, the 250-kDa SC polypeptide, which corresponds to S-2 protein (227 kDa), has less bsheet forming propensity and higher hydrophilicity than the 150-and 400-kDa polypeptides. This is because the 250-kDa SC contains larger amounts of b-sheet breaking residues like Glu, glutamine (Gln) and Lys, and smaller amounts of bsheet favoring residues like threonine (Thr) and tyrosine (Tyr) compared to the 150-and 400-kDa SCs. 2)Historically, sericulture was widely carried out to support the silk spinning and weaving industry in Japan. SC was degraded into low molecular weight fragments by a silk scouring process and released as a waste product.1) However, recent progress in transgenic technology has produced strains of transgenic silkworms that secrete recombinant proteins such as vaccines and cytokines in the SC of their cocoons. 7,8) Furthermore, the modification of silk protein properties by transgenic silkworms is now being attempted. Takabayashi reported the applicability of silk fibroin, expressed with a cell attachment factor, in the construction of biocompatible and biodegradable artificial blood vessels.9,10) Thus, modified silk protein as a functional material and a generator of recombinant protein is a promising new direction in the field of sericulture.In a study of the application of SC as a biomaterial, SC was reported to enhance the attachment of cultured human skin fibroblasts.11) In addition, SC hydrogel sheets containing a mixture of water ...

show abstract

“…This phenomenon could be described based on polyion complexation. 35,36 Polyion complexes are formed by electrostatic interactions between positively charged and negatively charged species. The GDNF was positively charged, and PLGA and gelatin type A were negatively charged.…”

Section: Article Wileyonlinelibrarycom/appmentioning

confidence: 99%

Preparation of bioactive glycosylated glial cell‐line derived neurotrophic factor‐loaded microspheres for medical applications

Thumsing

Israsena

Boonkrai

et al. 2013

J of Applied Polymer Sci

View full text Add to dashboard Cite

Poly (lactic-co-glycolic acid) (PLGA)-coated gelatin microspheres containing glial cell-line derived neurotrophic factor (GDNF) were developed by thermal gelation through a water-in-oil emulsion technique. Gelatin types (A and B) at four different pH levels were investigated for their influences on the morphology, the microsphere size, the zeta potential, and the swelling ability. The encapsulation of GDNF and the release characteristics of GDNF were also determined using enzyme-linked immunosorbent assay (ELISA). The maximum cumulative released amounts of GDNF from the microspheres were increased from 50 to 90% after 4 d (based on the actual amount of the GDNF). Thus, the release of the GDNF contents in the microspheres depends on the amount of GDNF. Trigeminal ganglion cells (TGCs) were used to study the bioactivity of GDNF released from the microspheres, which was proven to retain its bioactivity in promoting the TGCs' neurite outgrowth.

show abstract

Controlled release of plasmid DNA from hydrogels prepared from gelatin cationized by different amine compounds

Cited by 50 publications

References 31 publications

Hybrid Nanoparticle Design Based on Cationized Gelatin and the Polyanions Dextran Sulfate and Chondroitin Sulfate for Ocular Gene Therapy

Hybrid Nanoparticle Design Based on Cationized Gelatin and the Polyanions Dextran Sulfate and Chondroitin Sulfate for Ocular Gene Therapy

Use of Silk Protein, Sericin, as a Sustained-Release Material in the Form of a Gel, Sponge and Film

Preparation of bioactive glycosylated glial cell‐line derived neurotrophic factor‐loaded microspheres for medical applications

Contact Info

Product

Resources

About