Controlled- or slow-release urea can improve crop nitrogen
use
efficiencies and yields in many agricultural production systems. The
effect of controlled-release urea on the relationships between levels
of gene expression and yields has not been adequately researched.
We conducted a 2 year field study with direct-seeded rice, which included
treatments of controlled-release urea at four rates (120, 180, 240,
and 360 kg N ha–1), a standard urea treatment (360
kg N ha–1), and a control treatment without applied
nitrogen. Controlled-release urea improved the inorganic nitrogen
concentrations of root-zone soil and water, functional enzyme activities,
protein contents, grain yields, and nitrogen use efficiencies. Controlled-release
urea also improved the gene expressions of nitrate reductase [NAD(P)H]
(EC 1.7.1.2), glutamine synthetase (EC 6.3.1.2), and glutamate synthase
(EC 1.4.1.14). With the exception of glutamate synthase activity,
there were significant correlations among these indices. The results
showed that controlled-release urea improved the content of inorganic
nitrogen within the rice root zone. Compared with urea, the average
enzyme activity of controlled-release urea increased by 50–200%,
and the relative gene expression was increased by 3–4 times
on average. The added soil nitrogen increased the level of gene expression,
allowing enhanced synthesis of enzymes and proteins for nitrogen absorption
and use. Hence, controlled-release urea improved the nitrogen use
efficiency and the grain yield of rice. Controlled-release urea is
an ideal nitrogen fertilizer showing great potential for improving
rice production.