Controlled Rotation and Vibration of Patterned Cell Clusters Using Dielectrophoresis

Soffe, Rebecca; Tang, Shi‐Yang; Baratchi, Sara; Nahavandi, Sofia; Nasabi, Mahyar; Cooper, Jonathan M.; Mitchell, Arnan; Khoshmanesh, Khashayar

doi:10.1021/ac5043335

Cited by 26 publications

(16 citation statements)

References 44 publications

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“…Dielectrophoresis, the induced motion of polarisable particles such as cells under the influence of non-uniform electric fields, enables the label-free, selective and quick immobilisation of cells in microfluidic systems 16 17 26 27 28 . Despite these advantages, the long-term exposure of cells to strong electric fields may affect the viability, and functioning of cells 17 .…”

mentioning

confidence: 99%

Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis

Soffe

Baratchi

Tang

et al. 2015

Sci Rep

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Immobilisation of cells is an important feature of many cellular assays, as it enables the physical/chemical stimulation of cells; whilst, monitoring cellular processes using microscopic techniques. Current approaches for immobilising cells, however, are hampered by time-consuming processes, the need for specific antibodies or coatings, and adverse effects on cell integrity. Here, we present a dielectrophoresis-based approach for the robust immobilisation of cells, and analysis of their responses under high shear flows. This approach is quick and label-free, and more importantly, minimises the adverse effects of electric field on the cell integrity, by activating the field for a short duration of 120 s, just long enough to immobilise the cells, after which cell culture media (such as HEPES) is flushed through the platform. In optimal conditions, at least 90% of the cells remained stably immobilised, when exposed to a shear stress of 63 dyn/cm2. This approach was used to examine the shear-induced calcium signalling of HEK-293 cells expressing a mechanosensitive ion channel, transient receptor potential vaniloid type 4 (TRPV4), when exposed to the full physiological range of shear stress.

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confidence: 99%

Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis

Soffe

Baratchi

Tang

et al. 2015

Sci Rep

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“…However, the simulation in the work only considers the hydrodynamic stress tensor and the Maxwell stress tensor. In order to model rather complex structures in future, some other forces or moments involved should be included in our model [ 34 ], especially if lubricants such as bovine serum albumin (BSA) are coating cells.…”

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confidence: 99%

Numerical Investigation of DC Dielectrophoretic Deformable Particle–Particle Interactions and Assembly

Zhou

et al. 2018

Micromachines

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In a non-uniform electric field, the surface charge of the deformable particle is polarized, resulting in the dielectrophoretic force acting on the surface of the particle, which causes the electrophoresis. Due to dielectrophoretic force, the two deformable particles approach each other, and distort the flow field between them, which cause the hydrodynamic force correspondingly. The dielectrophoresis (DEP) force and the hydrodynamic force together form the net force acting on the particles. In this paper, based on a thin electric double layer (EDL) assumption, we developed a mathematical model under the arbitrary Lagrangian–Eulerian (ALE) numerical approach method to simulate the flow field, electric field, and deformable particles simultaneously. Simulation results show that, when two deformable particles’ distances are in a certain range, no matter the initial position of the two particles immersed in the fluid field, the particles will eventually form a particle–particle chain parallel to the direction of the electric field. In actual experiments, the biological cells used are deformable. Compared with the previous study on the DEP motion of the rigid particles, the research conclusion of this paper provides a more rigorous reference for the design of microfluidics.

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“…The two planar electrodes, interplaying with the polarized cells in between, can cause single cells to rotate. The mechanism works because cells are dielectric substance, which can be polarized under the action of AC electric fields generated by the two planar electrodes, and in turn affect the distribution of electric fields . Leveraging this characteristic, we proposed a microfluidic chip that can rotate single cells using only two planar electrodes as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

A cell electro‐rotation micro‐device using polarized cells as electrodes

Huang

Wang

2018

Electrophoresis

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Cell rotation is widely required in various fields as an important technique for single cell manipulation. Usually, the electro‐rotational manipulation of single cells by dielectrophoresis technologies requires at least three electrodes to generate rotating electric fields which induce cells to rotate. Here, we present a novel microfluidic chip capable of rotating single cell using only two planar electrodes by taking polarized cells as the extra electrodes with phase‐shifted signal. To demonstrate this idea, we configured two parallel and planar electrodes as basic dielectrophoresis elements and placed trenches above these electrodes to attract cells, which were in turn polarized to be electrodes. Through simulation, we confirmed the functional structure of the device works well to generate proper rotating electric fields for cell rotation. Through experiment, we successfully demonstrated controlled electro‐rotation of HeLa and HepaRG cells. The novel electro‐rotation mechanism not only simplifies the micro‐device structure but also reduces the complexity of single cell rotation operation which will be a benefit to the potential users.

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Controlled Rotation and Vibration of Patterned Cell Clusters Using Dielectrophoresis

Cited by 26 publications

References 44 publications

Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis

Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis

Numerical Investigation of DC Dielectrophoretic Deformable Particle–Particle Interactions and Assembly

A cell electro‐rotation micro‐device using polarized cells as electrodes

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