Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method

Howell, W. Mike; Black, D. Ann

doi:10.1007/bf01953855

Cited by 2,746 publications

(1,687 citation statements)

References 10 publications

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“…Chromosome preparation was conducted by squash technique from kidney cell (Chen andEbeling 1968, Nanda et al 1995). The chromosomes were stained with 10% Giemsa s for 30 min and identified for NORs by Ag-NOR staining (Howell and Black 1980). The length of short arm (Ls) and long arm (Ll) chromosome were measured and calculated for the length of total arm chromosome (LT, LT=Ls+Ll).…”

Section: Methodsmentioning

confidence: 99%

First Report of Chromosome Analysis of Saddleback Anemonefish, Amphiprion polymnus (Perciformes, Amphiprioninae), in Thailand

et al. 2012

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SummaryWe report the first chromosome analysis for the saddleback anemonefish (Amphiprion polymnus) from Thailand. Kidney cell samples were taken from 5 male and 5 female fish. The mitotic chromosome preparation was prepared by directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of A. polymnus was 2n=48 and the fundamental numbers (NF) was 96 in both male and female. The types of chromosomes were 6 large submetacentric, 2 large acrocentric, 10 medium metacentric, 12 medium submetacentric, 6 medium acrocentric, 10 small metacentric, and 2 small submetacentric chromosomes. The region adjacent to the telomeres on the short arms of chromosome pair 21 showed clearly observable secondary constriction/NORs. The karyotype formula for A. polymnus could be deduced as:2n (48

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Section: Methodsmentioning

confidence: 99%

First Report of Chromosome Analysis of Saddleback Anemonefish, Amphiprion polymnus (Perciformes, Amphiprioninae), in Thailand

et al. 2012

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“…Preparation of fish chromosomes was from kidney cells (Chen andEhbeling 1968, Nanda et al 1995). The chromosomes were stained with 10% Giemsa s for 30 min and identified for NORs by Ag-NOR staining (Howell and Black 1980). Metaphase figures were analyzed according to the chromosome classification of Chaiyasut (1989).…”

Section: Methodsmentioning

confidence: 99%

Standardized Karyotype and Idiogram of Two-Spot Glass Catfish, Ompok bimaculatus (Siluriformes, Siluridae) in Thailand by Conventional and Ag-NOR Staining Techniques

et al. 2012

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Summary Standardized karyotype and idiogram of the two-spot glass catfish (Ompok bimaculatus) from Chi basin, Maha Sarakham Province, northeast Thailand, were studied. Kidney cell samples were taken from 5 male and 5 female fish. The mitotic chromosome preparations were prepared directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of O. bimaculatus was 2n=50, the fundamental number (NF) was 90 in both males and females. The types of chromosomes were 10 large metacentric, 2 large submetacentric, 4 large acrocentric, 2 large telocentric, 4 medium metacentric, 18 medium submetacentric, 2 medium acrocentric, 2 medium telocentric, and 6 small telocentric chromosomes. The region adjacent to telomere of the short arm of submetacentric chromosome pair 4 showed clearly observable nucleolar organizer regions (NORs

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“…Conventional staining was performed with Giemsa diluted in phosphate buffer, pH 6.8. Ag-OR staining was obtained by the technique of HOWELL & BLACK (1980) and C-banding patterns, according to SUMNER (1972).…”

Section: Methodsmentioning

confidence: 99%

Cytogenetic analysis of Cycloramphus boraceiensis Heyer (Anura, Leptodactylidae)

2001

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Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method

Abstract: A 1-step silver-staining technique, requiring only 2 min to perform, is described for the differential staining of nucleolus organizer regions. A protective colloidal developer is used to control the reduction of the silver.

Cited by 2,746 publications

References 10 publications

First Report of Chromosome Analysis of Saddleback Anemonefish, <i>Amphiprion polymnus</i> (Perciformes, Amphiprioninae), in Thailand

First Report of Chromosome Analysis of Saddleback Anemonefish, <i>Amphiprion polymnus</i> (Perciformes, Amphiprioninae), in Thailand

Standardized Karyotype and Idiogram of Two-Spot Glass Catfish, <i>Ompok bimaculatus</i> (Siluriformes, Siluridae) in Thailand by Conventional and Ag-NOR Staining Techniques

Cytogenetic analysis of Cycloramphus boraceiensis Heyer (Anura, Leptodactylidae)

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