Controlled transmission of African cassava mosaic virus (ACMV) by Bemisia tabaci from cassava (Manihot esculenta Crantz) to seedlings of physic nut (Jatropha curcas L.)

Amoatey, H. M.; Appiah, Andrew Sarkodie; Danso, K. E.; Amiteye, Samuel; Appiah, Robert; Klu, G.Y.P.; Owusu, George

doi:10.5897/ajb2013.12276

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…This disease manifests in substantial leaf damage, such as yellowing of the leaves and sap drainage, and it attacks the fruits, thus significantly reducing the yield of seeds. Whitefly (Bemisia tabaci) is the vector that carries the virus and transmits it to Jatropha plants, so the spread of the mosaic disease is primarily determined by the distribution of whitefly vectors and the density of the host plants [6,7].…”

Section: Introductionmentioning

confidence: 99%

Role of the whitefly maturation period on mosaic disease propagation in Jatropha curcas plant

Basir

2023

Front. Appl. Math. Stat.

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Mosaic disease in Jatropha curcas plants is caused by begomoviruses carried by whitefly vectors, and only mature vectors can transmit the virus. In this study, a mathematical model is developed for the dynamic analysis of the spread of mosaic disease in the J. curcas plantation, accounting for the whitefly maturation period as a time delay factor. The existence conditions and stability of the equilibrium points have been studied with qualitative theory. The basic reproduction number, R0, is determined to study the stability of the disease-free equilibrium with respect to it. Transcritical bifurcation of the disease-free equilibrium and Hopf bifurcation of the endemic equilibrium are also analyzed. Using numerical simulations, the analytical findings are verified and discussed the different dynamical behaviors of the system. In this research, the stabilizing role of maturation delay has been established. That means when maturation time is large, disease will be transmitted when the infection rate is high.

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Section: Introductionmentioning

confidence: 99%

Role of the whitefly maturation period on mosaic disease propagation in Jatropha curcas plant

Basir

2023

Front. Appl. Math. Stat.

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Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

Charoenvilaisiri

Seepiban

Kumpoosiri

et al. 2021

Virol J

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Background Cassava mosaic disease (CMD) is one of the most devastating viral diseases for cassava production in Africa and Asia. Accurate yet affordable diagnostics are one of the fundamental tools supporting successful CMD management, especially in developing countries. This study aimed to develop an antibody-based immunoassay for the detection of Sri Lankan cassava mosaic virus (SLCMV), the only cassava mosaic begomovirus currently causing CMD outbreaks in Southeast Asia (SEA). Methods Monoclonal antibodies (MAbs) against the recombinant coat protein of SLCMV were generated using hybridoma technology. MAbs were characterized and used to develop a triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) for SLCMV detection in cassava leaves and stems. Assay specificity, sensitivity and efficiency for SLCMV detection was investigated and compared to those of a commercial ELISA test kit and PCR, the gold standard. Results A TAS-ELISA for SLCMV detection was successfully developed using the newly established MAb 29B3 and an in-house polyclonal antibody (PAb) against begomoviruses, PAb PK. The assay was able to detect SLCMV in leaves, green bark from cassava stem tips, and young leaf sprouts from stem cuttings of SLCMV-infected cassava plants without cross-reactivity to those derived from healthy cassava controls. Sensitivity comparison using serial dilutions of SLCMV-infected cassava sap extracts revealed that the assay was 256-fold more sensitive than a commercial TAS-ELISA kit and 64-fold less sensitive than PCR using previously published SLCMV-specific primers. In terms of DNA content, our assay demonstrated a limit of detection of 2.21 to 4.08 × 106 virus copies as determined by quantitative real-time PCR (qPCR). When applied to field samples (n = 490), the TAS-ELISA showed high accuracy (99.6%), specificity (100%), and sensitivity (98.2%) relative to the results obtained by the reference PCR. SLCMV infecting chaya (Cnidoscolus aconitifolius) and coral plant (Jatropha multifida) was also reported for the first time in SEA. Conclusions Our findings suggest that the TAS-ELISA for SLCMV detection developed in this study can serve as an attractive tool for efficient, inexpensive and high-throughput detection of SLCMV and can be applied to CMD screening of cassava stem cuttings, large-scale surveillance, and screening for resistance.

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Controlled transmission of African cassava mosaic virus (ACMV) by Bemisia tabaci from cassava (Manihot esculenta Crantz) to seedlings of physic nut (Jatropha curcas L.)

Cited by 2 publications

References 22 publications

Role of the whitefly maturation period on mosaic disease propagation in Jatropha curcas plant

Role of the whitefly maturation period on mosaic disease propagation in Jatropha curcas plant

Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

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