Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement

Xu, Rui; Xia, Hesheng; Wang, He; Li, Zhichao; Zhao, Jian; Bo, Liu; Wang, Yuzhen; Qiang, Lei; Kong, Yi; Bai, Yang; Yao, Zhen; Yan, Rongshuai; Li, Haisheng; Zhan, Rong; Yang, Sisi; Luo, Gaoxing; Wu, Jun

doi:10.1038/srep24596

Cited by 274 publications

(186 citation statements)

References 28 publications

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“…High values WVT cause dryness of the wound, while small rates lead to exudate accumulation . The WVT value for healthy skin is ~0.85 mg cm −2 h −1 , for injured skin, it can range from 1.16 mg cm −2 h −1 , in the case of a first‐degree burn, to 21.41 mg cm −2 h −1 for an open wound . Dressings made of chitosan and alginate complexes presented WVT values ranging from 2.36 to 3.88 mg cm −2 h −1 , which are similar to those of commercial wound dressing .…”

Section: Resultssupporting

confidence: 54%

Physicochemical and antimicrobial properties of in situ crosslinked alginate/hydroxypropyl methylcellulose/ε‐polylysine films

Blachechen

Petri

2019

J of Applied Polymer Sci

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In the present study, in situ crosslinked films were easily prepared by the spray deposition of Phase A, composed by sodium alginate (Alg), hydroxypropyl methylcellulose (HPMC), ε‐polylysine (ε‐PL), and plasticizers, followed by the spray deposition of Phase B (Ca2+ ions) on polystyrene dishes. The effect of the amount of plasticizer, HPMC, and ε‐PL in Phase A on the films properties was explored in a set of 12 systems. Fourier transform infrared vibrational spectroscopy in the attenuated total reflectance mode revealed that plasticizers compete with Ca2+ ions for complexation with Alg. Films with high HPMC content and low content of plasticizer and ε‐PL presented the best mechanical properties. The best system presented Young modulus of 0.76 ± 0.10 GPa, water vapor transmission of 5.35 ± 0.28 mg cm−2 h−1, swelling ratio of 1374 ± 97%, 99.99% reduction against Gram‐negative Escherichia coli bacteria, and 99.9% reduction against Gram‐positive Staphylococcus aureus bacteria. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48832.

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Section: Resultssupporting

confidence: 54%

Physicochemical and antimicrobial properties of in situ crosslinked alginate/hydroxypropyl methylcellulose/ε‐polylysine films

Blachechen

Petri

2019

J of Applied Polymer Sci

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“…Moderate WVTR is recommended to offer a moist environment to accelerate healing. High WVTR may cause dryness of the wound, while low WVTR may cause storing of wound exudates which may result in retardation of the wound healing (Xu et al, 2016). The values of the WVTR for normal skin have been reported to be in the range 8.5 g/m 2 /h to 11.6 g/ m 2 /h (Gonzalez et al, 2012).…”

Section: Water Vapor Transmission Rate (Wvtr)mentioning

confidence: 99%

Biophysical investigation of curcumin based nanocomposite for wound dressing application

Kamel¹,

Soliman²,

Saleh³

et al. 2018

J App Pharm Sci

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Curcumin (cur) loaded polyvinyl alcohol (PVA), chitosan (Cs) and PVA/Cs blend (50:50) were prepared with different concentrations of curcumin (0, 5, 10, and 15 wt%) using the solution casting technique and studied for wound dressing application. Physical characterization of the composites achieved through dielectric spectroscopy (DS), fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and transmission electron microscope (TEM). The wound dressing characteristics were evaluated by the water vapor transmission rate (WVTR), antimicrobial studies and cell viability assay. The scanning electron microscope revealed good dispersion of the nanocurcumin in the matrix. The dielectric properties of all composites were studied through the measurements of the permittivity ε′ and dielectric loss ε″. These results show that both values for PVA/cur nanocomposites greatly increase with increasing curcumin content which was found to be higher than those of the two individuals. On the opposite side, both ε′ and ε″ decrease with increasing curcumin content in case of Cs/cur and PVA/Cs/cur nanocomposites. The obtained electrical conductivity for all composites lies in the order of (10 ). WVTR of the samples decreased by increasing the curcumin %. Results of the cell viability assay confirmed that the addition of the curcumin has no toxic effect on the cells. PVA/cur nanocomposites displayed excellent antimicrobial activity.

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“…Briefly, the dorsal surface of the mouse was shaved, and two sets of sterile full-thickness wounds were generated using a sterile 4-mm punch tool. The survey of wound healing was performed as previously reported with minor revision [23, 24]. Wounds were left uncovered, and mice were kept individually with sterile paper bedding.…”

Section: Methodsmentioning

confidence: 99%

“…Tissues were fixed with 4% formaldehyde, embedded in paraffin, and sectioned at a thickness of 5 µm. Hematoxylin and eosin (HE) staining and histological analyses were performed, and the length of the neo-epithelium was determined using IPP 6.0 software; the measurement procedures were performed blindly by two pathologists [23, 24]. Experiments were repeated 5 times.…”

Section: Methodsmentioning

confidence: 99%

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Differential Role of Rapamycin in Epidermis-Induced IL-15-IGF-1 Secretion via Activation of Akt/mTORC2

Bai¹,

Xu²,

Zhang³

et al. 2017

Cell Physiol Biochem

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Backgroud/Aims: The effects of rapamycin (RPM) on wound healing have been previously studied. However, reciprocal contradictory data have been reported, and the underlying mechanism remains unclear. This study aims to uncover differential role of RPM in regulation of wound healing and explore the possible mechanism. Methods: C57BL/6J mice and epidermal cells were treated with different doses of RPM. The wound re-epithelialization was observed by hematoxylin and eosin (HE) staining. The expression of IL-15 and IGF-1 were detected by immunohistochemistry and quantitative real-time PCR. Epidermal cell survival was determined by CCK-8 assays. Moreover, the mTORC1 and mTORC2 pathway were examined by western blot analysis. Results: This study showed that differential doses of RPM could lead to separate consequences in epidermis. Histological analyses showed that low-dose RPM promoted wound healing, and enhanced the expression of IL-15 and IGF-1. Furthermore, western blot analysis showed that the effect of low-dose RPM in epidermis were not through mTORC1 pathway. Instead, activation of the Akt/mTORC2 pathway was involved in low-dose RPM-induced IL-15 and IGF-1 production in epidermis, while high-dose RPM inhibited the expression of IL-15 and IGF-1 and the activity of mTORC1 and mTORC2 pathway. Conclusion: This study for the first time demonstrated that RPM-mediated wound healing was dose-dependent.

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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement

Cited by 274 publications

References 28 publications

Physicochemical and antimicrobial properties of in situ crosslinked alginate/hydroxypropyl methylcellulose/ε‐polylysine films

Physicochemical and antimicrobial properties of in situ crosslinked alginate/hydroxypropyl methylcellulose/ε‐polylysine films

Biophysical investigation of curcumin based nanocomposite for wound dressing application

Differential Role of Rapamycin in Epidermis-Induced IL-15-IGF-1 Secretion via Activation of Akt/mTORC2

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