Controlling antimicrobial activity and drug loading capacity of chitosan-based layer-by-layer films

Neto, João Batista Maia Rocha; Lima, Giulia Grassa; Fiamingo, Anderson; Germiniani, Luiz G. L.; Taketa, Thiago Bezerra; Bataglioli, Rogério Aparecido; Silveira, G.A.T. da; Silva, J.V.L. da; Filho, Sérgio Paulo Campana; Oliveira, Osvaldo N.; Beppu, Marisa Masumi

doi:10.1016/j.ijbiomac.2020.12.218

Cited by 22 publications

(10 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 22 , 23 The latest advances in chitosan-based antimicrobial materials revealed a potent anti-bacterial and anti-fungal capacity. 22–24 These data are in line with those of Rocha Neto et al 25 who reported that a bi-layer film of chitosan and hyaluronic acid allowed a potent anti- E. coli effect. Some researches highlighted also that artificial hydrogels modified with chitosan exerted antibacterial efficacy against both S. aureus and E. coli .…”

Section: Introductionsupporting

confidence: 87%

Antibacterial and Antifungal Efficacy of Medium and Low Weight Chitosan-Shelled Nanodroplets for the Treatment of Infected Chronic Wounds

Mandras¹,

Argenziano²,

Prato³

et al. 2022

IJN

View full text Add to dashboard Cite

Purpose Medium versus low weight (MW vs LW) chitosan-shelled oxygen-loaded nanodroplets (cOLNDs) and oxygen-free nanodroplets (cOFNDs) were comparatively challenged for biocompatibility on human keratinocytes, for antimicrobial activity against four common infectious agents of chronic wounds (CWs) – methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Candida albicans and C. glabrata – and for their physical interaction with cell walls/membranes. Methods cNDs were characterized for morphology and physico-chemical properties by microscopy and dynamic light scattering. In vitro oxygen release from cOLNDs was measured through an oximeter. ND biocompatibility and ability to promote wound healing in human normoxic/hypoxic skin cells were challenged by LDH and MTT assays using keratinocytes. ND antimicrobial activity was investigated by monitoring upon incubation with/without MW or LW cOLNDs/cOFNDs either bacteria or yeast growth over time. The mechanical interaction between NDs and microorganisms was also assessed by confocal microscopy. Results LW cNDs appeared less toxic to keratinocytes than MW cNDs. Based on cell counts, either MW or LW cOLNDs and cOFNDs displayed long-term antimicrobial efficacy against S. pyogenes, C. albicans , and C. glabrata (up to 24 h), whereas a short-term cytostatic effects against MRSA (up to 6 h) was revealed. The internalization of all ND formulations by all four microorganisms, already after 3 h of incubation, was showed, with the only exception to MW cOLNDs/cOFNDs that adhered to MRSA walls without being internalized even after 24 h. Conclusion cNDs exerted bacteriostatic and fungistatic effects, due to the presence of chitosan in the outer shell and independently of oxygen addition in the inner core. The duration of such effects strictly depends on the characteristics of each microbial species, and not on the molecular weight of chitosan in ND shells. However, LW chitosan was better tolerated by human keratinocytes than MW. For these reasons, the use of LW NDs should be recommended in future research to assess cOLND efficacy for the treatment of infected CWs.

show abstract

Section: Introductionsupporting

confidence: 87%

Antibacterial and Antifungal Efficacy of Medium and Low Weight Chitosan-Shelled Nanodroplets for the Treatment of Infected Chronic Wounds

Mandras¹,

Argenziano²,

Prato³

et al. 2022

IJN

View full text Add to dashboard Cite

show abstract

“…It is also important to emphasize that the outermost layer of the films is composed of HA, which reinforces the role of charge density and assumed conformation of this polyelectrolyte not only in the film topography, but also in all surface properties. The increase in the number of bilayers, in turn, reveals a kind of roughness saturation in the coatings, which have a growth profile based on the construction of polymeric islands [70], which was also described in previous literature reports [48,71].…”

Section: Atomic Force Microscopy (Afm)supporting

confidence: 75%

“…It was suggested that under significantly different pH conditions than those of film assembly, a change in the ionization profile of CHI and TA occurs, which compromises the electrostatic interaction between the molecules and, thus, leads to the disintegration of the multilayer films [73]. Moreover, recent investigations carried out by our group on the use of chitosan molecules with different degrees of deacetylation pointed to the control of CHI properties as a promising strategy to promote a higher stability of HA/CHI films under pH levels close to physiological conditions [48]. By adjusting experimental variables such as pH of the polyelectrolytic solutions and number of bilayers, it was possible to promote changes in the physical and chemical properties of HA/CHI multilayer films, changing the topographic profile, capacitance, thickness, availability of free functional groups, and hydrophilicity.…”

Section: Cell Adhesion Assays Selective Potential Of the Multilayer Filmsmentioning

confidence: 99%

“…It was suggested that under significantly different pH conditions than those of film assembly, a change in the ionization profile of CHI and TA occurs, which compromises the electrostatic interaction between the molecules and, thus, leads to the disintegration of the multilayer films [73]. Moreover, recent investigations carried out by our group on the use of chitosan molecules with different degrees of deacetylation pointed to the control of CHI properties as a promising strategy to promote a higher stability of HA/CHI films under pH levels close to physiological conditions [48]. Considering that, in this study, films developed under the same pH conditions were exposed in the same manner to the cell culture medium at physiological pH, we also suggest that the disintegration of the films can be aggravated by an increase in the number of bilayers, therefore corroborating with the results observed for films of 10.5 and 20.5 bilayers in cell adhesion assays.…”

Section: Cell Adhesion Assays Selective Potential Of the Multilayer Filmsmentioning

confidence: 99%

“…Thus, by using oppositely charged polyelectrolytes, it is possible to build multilayer films via Layer-bylayer (LbL) deposition, in such a way that the cohesion between the layers is maintained by electrostatic forces. Films based on HA and CHI have been used in several applications, such as antimicrobial surfaces [45,46], cell adhesion [47], drug delivery [48], biosensing [5], wound dressings [29,49], gene delivery systems [50,51], and tissue engineering [52].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture

et al. 2021

View full text Add to dashboard Cite

Prostate cancer (PCa) is a slow-growing neoplasm that has, when diagnosed in its early stages, great chances of cure. During initial tumor development, current diagnostic methods fail to have the desired accuracy, thus, it is necessary to develop or improve current detection methods and prognostic markers for PCa. In this scenario, films composed of hyaluronic acid (HA) and chitosan (CHI) have demonstrated significant capture potential of prostate tumor cells (PC3 line), exploring HA as a CD44 receptor ligand and direct mediator in cell-film adhesion. Here, we present a strategy to control structural and cell adhesion properties of HA/CHI films based on film assembly conditions. Films were built via Layer-by-layer (LbL) deposition, where the pH conditions (3.0 and 5.0) and number of bilayers (3.5, 10.5, and 20.5) were controlled. The characterization of these films was carried out using profilometry, ultraviolet-visible (UV-VIS), atomic force microscopy (AFM) and contact angle measurements. Multilayer HA/CHI films produced at pH 3.0 gave optimum surface wettability and availability of free carboxyl groups. In turn, at pH 5.0, the coverings were thinner and presented a smoother surface. Films prepared with 3.5 bilayers showed greater tumor cell capture regardless of the pH condition, while films containing 10.5 and 20.5 bilayers presented a significant swelling process, which compromised their cell adhesion potential. This study shows that surface chemistry and morphology are critical factors for the development of biomaterials designed for several cell adhesion applications, such as rapid diagnostic, cell signaling, and biosensing mechanisms.

show abstract

Construction of antifouling marine coatings via layer-by-layer assembly of chitosan and acid siloxane resin

et al. 2023

View full text Add to dashboard Cite

Controlling antimicrobial activity and drug loading capacity of chitosan-based layer-by-layer films

Cited by 22 publications

References 48 publications

Antibacterial and Antifungal Efficacy of Medium and Low Weight Chitosan-Shelled Nanodroplets for the Treatment of Infected Chronic Wounds

Antibacterial and Antifungal Efficacy of Medium and Low Weight Chitosan-Shelled Nanodroplets for the Treatment of Infected Chronic Wounds

Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture

Construction of antifouling marine coatings via layer-by-layer assembly of chitosan and acid siloxane resin

Contact Info

Product

Resources

About