Controlling bacterial adhesion to surfaces using topographical cues: a study of the interaction of Pseudomonas aeruginosa with nanofiber-textured surfaces

Kargar, Mehdi; Ji, Wang; Nain, Amrinder S.; Behkam, Bahareh

doi:10.1039/c2sm26368h

Cited by 62 publications

(77 citation statements)

References 20 publications

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“…Many groups have fabricated engineered surface topography, using numerous fabrication techniques (soft lithography and double casting molding techniques, microcontact printing, electron beam lithography, nanoimprint lithography, photolithography, electrodeposition methods, etc.) [37][38][39][40][41][42][43][44][45][46][47] on a wide range of substrates, ranging from various polymeric materials (silicone, polystyrene, polyurethane, and epoxy resins) to metals and metal oxides (silicon, titanium, aluminum, silica, and gold) [21,40,45,[47][48][49][50][51][52][53][54][55]. Furthermore, engineered topography can be considered as part of a multi-faceted strategy to prevent biofouling, and could be combined with other methods such as surface chemical treatments, addition of antibiotics/bacteriostatic agents, etc.…”

Section: Surface Attachment and Biofilm Formationmentioning

confidence: 99%

“…Most of these approaches result in highly regular surface structures with a square or rectangular cross-sectional profile. Kargar et al introduced an alternative approach to antifouling surfaces by fabricating topographical features with curved tops, instead of the more typical flat-topped, rectangular profile features [52]. To fabricate these curved surface topographies, smooth polystyrene (PS) surfaces served as a substrate for a network of linearly arranged PS fibers.…”

Section: Modes Of Bacterial Surface Attachment/alternative Topographiesmentioning

confidence: 99%

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Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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Bacterial surface fouling is problematic for a wide range of applications and industries, including, but not limited to medical devices (implants, replacement joints, stents, pacemakers), municipal infrastructure (pipes, wastewater treatment), food production (food processing surfaces, processing equipment), and transportation (ship hulls, aircraft fuel tanks). One method to combat bacterial biofouling is to modify the topographical structure of the surface in question, thereby limiting the ability of individual cells to attach to the surface, colonize, and form biofilms. Multiple research groups have demonstrated that micro and nanoscale topographies significantly reduce bacterial biofouling, for both individual cells and bacterial biofilms. Antifouling strategies that utilize engineered topographical surface features with well-defined dimensions and shapes have demonstrated a greater degree of controllable inhibition over initial cell attachment, in comparison to undefined, texturized, or porous surfaces. This review article will explore the various approaches and techniques used by researches, including work from our own group, and the underlying physical properties of these highly structured, engineered micro/nanoscale topographies that significantly impact bacterial surface attachment.

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Section: Surface Attachment and Biofilm Formationmentioning

confidence: 99%

Section: Modes Of Bacterial Surface Attachment/alternative Topographiesmentioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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“…3d). This assembly phenomenon was likely driven by the cells tendency to maximize their contact area with the surface, a hypothesis supported by experiments showing reduced adhesion upon addition of a micro-texture that minimized cell-surface contact area (Kargar et al 2012). Further work in this area could result in materials with controlled surface topography with broad implications for anti-biofouling and other biomedical and industrial applications.…”

Section: A World With Boundariesmentioning

confidence: 94%

Microfluidics Expanding the Frontiers of Microbial Ecology

Rusconi

Garren

Stocker

2014

Annu. Rev. Biophys.

177

146

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The ability afforded by microfluidics to observe the behaviors of microbes in highly controlled and confined microenvironments, across scales from a single cell to mixed communities, has significantly contributed to expand the frontiers of microbial ecology over the last decade. Spatially and temporally varying distributions of organisms and chemical cues that mimic natural microbial habitats can now be established by exploiting physics at the micrometer scale and by incorporating structures with specific geometries and materials. Here we review applications of microfluidics that have resulted in highly insightful discoveries on fundamental aspects of microbial life, ranging from growth and sensing to cell-cell interactions and population dynamics. We anticipate that this flexible, multidisciplinary technology will continue to facilitate discoveries regarding the ecology of microorganisms and help uncover strategies to control phenomena such as biofilm formation and antibiotic resistance.

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“…[11][12][13] Bacterial adhesion is affected by the contact area of a cell on a surface. 14 Previous studies reported that more cells were observed where there were larger contact area. [15][16][17] In addition to the contract area, the surface morphology should play a role.…”

Section: Introductionmentioning

confidence: 99%

Bacteria repelling on highly-ordered alumina-nanopore structures

Kim

Zhou

Cirillo

et al. 2015

Journal of Applied Physics

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Bacteria introduce diseases and infections to humans by their adherence to biomaterials, such as implants and surgical tools. Cell desorption is an effective step to reduce such damage. Here, we report mechanisms of bacteria desorption. An alumina nanopore structure (ANS) with pore size of 35 nm, 55 nm, 70 nm, and 80 nm was used as substrate to grow Escherichia coli (E. coli) cells. A bacteria repelling experimental method was developed to quantitatively evaluate the area percentage of adherent bacterial cells that represent the nature of cell adhesion as well as desorption. Results showed that there were two crucial parameters: contact angle and contact area that affect the adhesion/desorption. The cells were found to be more easily repelled when the contact angle increased. The area percentage of adherent bacterial cells decreased with the decrease in the contact area of a cell on ANS. This means that cell accessibility on ANS depends on the contact area. This research reveals the effectiveness of the nanopored structures in repelling cells. V C 2015 AIP Publishing LLC. [http://dx

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Controlling bacterial adhesion to surfaces using topographical cues: a study of the interaction of Pseudomonas aeruginosa with nanofiber-textured surfaces

Cited by 62 publications

References 20 publications

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Microfluidics Expanding the Frontiers of Microbial Ecology

Bacteria repelling on highly-ordered alumina-nanopore structures

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