Controlling cell elongation and orientation by using microstructural nanofibre scaffolds for accelerating tissue regeneration

Razali, Nur Adila Mohd; Lin, Wei-Chih; Norzain, Norul Ashikin; Yu, Zhiwei

doi:10.1016/j.msec.2021.112321

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…[ 45,66 ] The good guidance of the aligned structure promotes cell elongation. [ 67,68 ] He et.al reported that huntingtin‐regulated lysosome activity is a potential key factor for determining SCs growth behavior on oriented surface. [ 69 ]…”

Section: Resultsmentioning

confidence: 99%

Properties of Electrospun Aligned Poly(lactic acid)/Collagen Fibers with Nanoporous Surface for Peripheral Nerve Tissue Engineering

Zhang

Dai

2022

Macro Materials & Eng

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It is important for the functional recovery of defective nerves to construct a suitable microenvironment for nerve regeneration by optimizing the topological structure and biological functions of nerve grafts. Electrospun fibers are employed to mimic the extracellular matrix (ECM) and the effect of the porous features on fiber surface draws much attention. Yet, little is known about the effect of the nanotopology of the fiber surface in conjunction with collagen I (COL) on neural cells. Herein, poly(lactic acid) (PLA)/COL hybrid fibrous membranes with a nanoporous structure on a fiber surface are fabricated. The performance of the obtained fibrous membranes is investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), water contact angle (WCA), and tensile test measurements. The cytotoxicity and biocompatibility of PLA/COL hybrid fibrous membranes and their effects on neural cells are systematically investigated. The physicochemical test results show that the PLA/COL hybrid fibrous membranes have appropriate mechanical properties and improved hydrophilicity. The MTT assay and hemolysis assay suggest the nontoxicity and good hemocompatibility of PLA/COL membranes. The nanoporous structure on the fiber surface and the introduction of COL synergistically promote proliferation and elongation of Schwann cells (SCs) and the extension of dorsal root ganglion (DRG) neurites.

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Section: Resultsmentioning

confidence: 99%

Properties of Electrospun Aligned Poly(lactic acid)/Collagen Fibers with Nanoporous Surface for Peripheral Nerve Tissue Engineering

Zhang

Dai

2022

Macro Materials & Eng

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“…Finally, the as-stained samples were observed using confocal laser scanning microscopy (CLSM, Zeiss 900 CLSM). The cell aspect ratio, which is the ratio between the major and minor axes of a fitted ellipse, was calculated to evaluate the cell elongation according to the previous study [21]. In addition, after 14 days of culture, the cell-seeded scaffolds were fixed using 2.5% glutaraldehyde and dehydrated with increasing concentrations of ethanol.…”

Section: Cellular Morphology Viability and Phenotype Evaluationmentioning

confidence: 99%

Constructing high-strength nano-micro fibrous woven scaffolds with native-like anisotropic structure and immunoregulatory function for tendon repair and regeneration

Cai

Liu

et al. 2023

Biofabrication

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Tendon injuries are common debilitating musculoskeletal diseases with high treatment expenditure in sports medicine. The development of tendon-biomimetic scaffolds may be promising for improving the unsatisfactory clinical outcomes of traditional therapies. In this study, we combined an advanced electrospun nanofiber yarn-generating technique with a traditional textile manufacturing strategy to fabricate innovative nano-micro fibrous woven scaffolds with tendon-like anisotropic structure and high-strength mechanical properties for the treatment of large-size tendon injury. Electrospun nanofiber yarns made from pure poly L-lactic acid (PLLA) or silk fibroin (SF)/PLLA blend were fabricated, and their mechanical properties matched and even exceeded those of commercial PLLA microfiber yarns. The PLLA or SF/PLLA nanofiber yarns were then employed as weft yarns interlaced with commercial PLLA microfiber yarns as warp yarns to generate two new types of nanofibrous scaffolds (nmPLLA and nmSF/PLLA) with a plain-weaving structure. Woven scaffolds made from pure PLLA microfiber yarns (both weft and warp directions) (mmPLLA) were used as controls. In vitro experiments showed that the nmSF/PLLA woven scaffold with aligned fibrous topography significantly promoted cell adhesion, elongation, proliferation, and phenotypic maintenance of tenocytes compared with mmPLLA and nmPLLA woven scaffolds. Moreover, the nmSF/PLLA woven scaffold exhibited the strongest immunoregulatory functions and effectively modulated macrophages towards the M2 phenotype. In vivo experiments revealed that the nmSF/PLLA woven scaffold notably facilitated Achilles tendon regeneration with improved structure by macroscopic, histological, and ultrastructural observations 6 months after surgery, compared with the other two groups. More importantly, the regenerated tissue in the nmSF/PLLA group had excellent biomechanical properties comparable to those of the native tendon. Overall, our study provides an innovative biological-free strategy with ready-to-use features, which presents great potential for clinical translation for damaged tendon repair.

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“…21 In addition to promoting cellular motility and morphological changes at wound sites, the topographic surface conditions of scaffolds also impact cellular behavior by promoting tissue regeneration. 48 Controlling the shape and morphology of fibers and selecting combinations that do not disrupt regular physiological function are critical for the production of scaffolds in tissue engineering applications. Collagen, a wellknown biopolymer, is commonly used in electrospinning nanofiber compositions.…”

Section: Therapeutic Applications Of Nanofibersmentioning

confidence: 99%

Evolving Trends in Nanofibers for Topical Delivery of Therapeutics in Skin Disorders

Tomar,

Pandit,

Priya

et al. 2023

ACS Omega

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Nanotechnology has yielded nanostructure-based drug delivery approaches, among which nanofibers have been explored and researched for the potential topical delivery of therapeutics. Nanofibers are filaments or thread-like structures in the nanometer size range that are fabricated using various polymers, such as natural or synthetic polymers or their combination. The size or diameter of the nanofibers depends upon the polymers, the techniques of preparation, and the design specification. The four major processing techniques, phase separation, self-assembly, template synthesis, and electrospinning, are most commonly used for the fabrication of nanofibers. Nanofibers have a unique structure that needs a multimethod approach to study their morphology and characterization parameters. They are gaining attention as drug delivery carriers, and the substantially vast surface area of the skin makes it a potentially promising strategy for topical drug products for various skin disorders such as psoriasis, skin cancers, skin wounds, bacterial and fungal infections, etc. However, the large-scale production of nanofibers with desired properties remains challenging, as the widely used electrospinning processes have certain limitations, such as poor yield, use of high voltage, and difficulty in achieving in situ nanofiber deposition on various substrates. This review highlights the insights into fabrication strategies, applications, recent clinical trials, and patents of nanofibers for different skin disorders in detail. Additionally, it discusses case studies of its effective utilization in the treatment of various skin disorders for a better understanding for readers.

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Controlling cell elongation and orientation by using microstructural nanofibre scaffolds for accelerating tissue regeneration

Cited by 14 publications

References 33 publications

Properties of Electrospun Aligned Poly(lactic acid)/Collagen Fibers with Nanoporous Surface for Peripheral Nerve Tissue Engineering

Properties of Electrospun Aligned Poly(lactic acid)/Collagen Fibers with Nanoporous Surface for Peripheral Nerve Tissue Engineering

Constructing high-strength nano-micro fibrous woven scaffolds with native-like anisotropic structure and immunoregulatory function for tendon repair and regeneration

Evolving Trends in Nanofibers for Topical Delivery of Therapeutics in Skin Disorders

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