Controlling Citrus Huanglongbing: Green Sustainable Development Route Is the Future

Li, Xue; Ruan, Huaqin; Zhou, Chengqian; Meng, Xiangchun; Chen, Wenli

doi:10.3389/fpls.2021.760481

Cited by 30 publications

(18 citation statements)

References 156 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Citrus greening or huanglongbing (HLB) has reduced Florida citrus production by >75% since 2003 and has severely impacted other citrus growing regions around the world ( Gottwald, 2010 ; Li et al, 2021 ). The fruit yield from infected trees is dramatically decreased and infected trees ultimately die.…”

Section: Introductionmentioning

confidence: 99%

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

et al. 2022

View full text Add to dashboard Cite

The curry leaf tree, Bergera koenigii, is highly attractive to the Asian citrus psyllid, Diaphorina citri, which vectors the bacterial causative agent of citrus greening or huanglongbing disease. This disease has decimated citrus production in Florida and in other citrus-producing countries. As D. citri exhibits high affinity for feeding on young leaves of B. koenigii, transgenic B. koenigii expressing bacteria-derived pesticidal proteins such as Cry1Ba1 have potential for D. citri management when planted in or adjacent to citrus groves. Importantly, the plant pathogenic bacterium that causes citrus greening does not replicate in B. koenigii. Transgenic plants of B. koenigii were produced by insertion of the gene encoding the active core of the pesticidal protein Cry1Ba1 derived from Bacillus thuringiensis. The transformation success rate was low relative to that of other citrus, at 0.89%. T-DNA integration into the genome and cry1ba1 transcription in transgenic plants were confirmed. Transgenic plants expressing Cry1Ba1 differed from wild-type plants, differed in photosynthesis parameters and hormone levels in some instances, and a marked delay in wilting of detached leaves. The gut epithelium of D. citri fed on transgenic plants was severely damaged, consistent with Cry1Ba1-mediated pore formation, confirming expression of the pesticidal protein by transgenic B. koenigii. These results demonstrate that transgenic B. koenigii expressing bacteria-derived pesticidal proteins can be produced for potential use as trap plants for suppression of D. citri populations toward protection of citrus groves from citrus greening.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…HLB disease is a major concern for many farmers, technicians and researchers around the world. Due to the di culty of CLas isolation (Merfa et al 2019), the development of strategies to directly attack the bacteria causing the disease have been rather slow, making the generation of genetic resistance to the pathogen the most promissory tool for long time-management of HLB (Martinelli and Dandekar, 2017;Li et al 2021). Hence, the study of different genotypes with different levels of HLB-tolerance is very important to understand the molecular mechanisms of this tolerance and then use it in breeding programs aiming the generation of HLB-tolerant genotypes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome wide identification of CDR family in Citrus latifolia and its expression during HLB infection

Rosa

González-Cruz

Adame-García

et al. 2022

Preprint

View full text Add to dashboard Cite

Huanglongbing (HLB) is the most important disease in citrus production around the world. Currently, there are not HLB-resistant citrus species or genotypes, however, Persian lime (Citrus latifolia Tan.) has been reported as the most tolerant citrus species to this disease, but their molecular mechanisms of tolerance has not being characterized yet. In other citrus species, Poncirus trifoliate, it has been suggested that the overexpression of CDR genes is associated to HLB tolerance, therefore, the aim of this work was to identify CDR gene family members in the Persian lime transcriptome and to determine if the HLB infection induces their expression. A Bayesian approach was used to search transcripts with one or two catalytic domains, then, a non-weighted parsimony phylogeny for CDR genes was carried out with the selected transcripts from C latifolia, P. trifoliata and C. sinensis, to identify phylogenetic homologous. The corresponding encoded proteins were obtained from the selected genes, and the similarity among them was estimated using alignments, identity matrix, UPGMA and conserved motifs analysis. Once the ClCDR genes were identified, their expression was determined with the transcriptome data and validated by RT-PCR and qRT-PCR in tissues of asymptomatic and HBL-symptomatic leaves. 146 sequences with one or two catalytic domains, were found, and 7 CDR clades were recovered. Similarity analysis demonstrated that there is a complex relationship among CDR genes and proteins in the species used in the present study. Moreover, the transcriptome and qRT-PCR data showed that none of the ClCDRgenes were expressed, neither in healthy nor in HLB-infected Persian lime plants, except for the ClCDR8a gene, which showed high expression. However, no significant differences were found between the ClCDR8a REL values from symptomatic or non-symptomatic leaves. Our results demonstrated the presence of CDR genes in the Persian lime genome, however, they suggest that HLB tolerance is not correlated with the overexpression of this gene family in this species.

show abstract

“…On the other hand, many effective phytosanitary products have been withdrawn or limited in their use due to their toxicity or environmental contamination [2-6]. Finally, new pathogen threats emerge continually, such as the huanglongbing (HLB) caused by the bacterium Xanthomonas citri, which has endangered citrus production worldwide in recent years [7].…”

Section: Introductionmentioning

confidence: 99%

Host-Defense Peptides as New Generation Phytosanitaries: Low Toxicity and Low Induction of Antimicrobial Resistance

Lobo

Boto

2022

Agronomy

View full text Add to dashboard Cite

Host-defense peptides (HDP) are emerging as promising phytosanitaries due to their potency, low plant, animal and environmental toxicity, and above all, low induction of antimicrobial resistance. These natural compounds, which have been used by animals and plants over millions of years to defend themselves against pathogens, are being discovered by genome mining, and then produced using biofactories. Moreover, truncated or otherwise modified peptides, including ultra-short ones, have been developed to improve their bioactivities and biodistribution, and also to reduce production costs. The synergistic combination of HDP and other antimicrobials, and the development of hybrid molecules have also given promising results. Finally, although their low induction of antimicrobial resistance is a big advantage, cautionary measures for the sustainable use of HDPs, such as the use of precision agriculture tools, were discussed.

show abstract

Controlling Citrus Huanglongbing: Green Sustainable Development Route Is the Future

Cited by 30 publications

References 156 publications

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

Genetic Modification of Bergera koenigii for Expression of the Bacterial Pesticidal Protein Cry1Ba1

Transcriptome wide identification of CDR family in Citrus latifolia and its expression during HLB infection

Host-Defense Peptides as New Generation Phytosanitaries: Low Toxicity and Low Induction of Antimicrobial Resistance

Contact Info

Product

Resources

About