Controlling hyperhydration of carnations (Dianthus caryophyllus L.) grown in a mist reactor

Correll, Melanie J.; Wu, Yingxia; Weathers, Pamela J.

doi:10.1002/1097-0290(2000)71:4<307::aid-bit1019>3.0.co;2-9

Cited by 26 publications

(11 citation statements)

References 44 publications

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“…Various factors have been enumerated responsible for hyper-hydration of in vitro cultures (Correll et al, 2001) including supra-optimal concentrations of cytokinins, increased water potential and hormonal imbalance in the medium. PGRs and mineral nutrient availability directly affect plant growth in vitro.…”

Section: Discussionmentioning

confidence: 99%

Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers

Rathore

Mastan

Yadav

et al. 2016

South African Journal of Botany

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Edited by J Van StadenKeywords: Endangered Ex vitro Leaf regeneration Vegetable rennet and withanolides Withania coagulans (Stocks) Dunal (Solanaceae) is a critically endangered medicinal plant known for its multiple medicinal properties. We report here an efficient and high frequency plantlet regeneration system through direct organogenesis in W. coagulans using in vitro-derived leaves as explants. On Murashige and Skoog's (MS) medium containing 4.44 μM 6-benzylaminopurine (BAP) 73.7 ± 4.3% explants responded and produced 11.4 ± 0.9 shoot buds per explant. The regenerated shoot buds were elongated (6.7 ± 0.22 cm) on MS medium with 1.11 μM BAP and 0.57 μM indole-3-acetic acid (IAA). The elongated shoots were rooted both in vitro and ex vitro. The regenerates were acclimatized by slow and gradual exposure to different regimes of temperature and relative humidity. The leaf-regenerates were assessed for genetic stability using RAPD and ISSR molecular markers and confirmed true-to-type. The in vitro regeneration system developed would be useful for genetic restoration program as the overexploitation and reproductive failure forced W. coagulans towards the verge of complete extinction. The regeneration method would also be useful for genetic transformation for genetic improvement and improved phyto-pharming.

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Section: Discussionmentioning

confidence: 99%

Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers

Rathore

Mastan

Yadav

et al. 2016

South African Journal of Botany

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“…The high incidence of hyperhydricity in liquid media has been attributed to low oxygen content, absence of gaseous exchange and suppressed transpiration (George 1993). High hyperhydricity was also reported in liquid cultures of apple (Marga et al 1997), carnation (Correll et al 2001) and Eucalyptus (Whitehouse et al 2002).…”

Section: Semi-solid Versus Liquid Mediummentioning

confidence: 94%

Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla

Ivanova

Staden

2010

Plant Cell Tiss Organ Cult

125

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Shoot regeneration and occurrence of hyperhydricity in Aloe polyphylla were greatly affected by the type of gelling agent. The use of gelrite resulted in a significantly lower multiplication and almost four times higher hyperhydricity (65%) compared to agar-solidified medium. Gelrite was further selected to evaluate if hyperhydricity can be overcome by altering the physical properties of the gel, as represented by increasing gelrite concentrations. Four concentrations of gelrite (0, 2.4, 6 and 16 g l -1 ) were tested in combination with zeatin, N 6 -benzyladenine (BA) or thidiazuron (TDZ). Almost all explants grown in liquid media in the presence of cytokinins became hyperhydric and lost their ability to regenerate. The greatest shoot formation was obtained on media with 2.4 g l -1 gelrite and 5 lM zeatin or BA, however hyperhydricity was very high. Satisfactory reduction in hyperhydricity was achieved only at 16 g l -1 gelrite, under which conditions the multiplication also decreased. The use of TDZ resulted in very low shoot regeneration and high hyperhydricity irrespective of the gelrite concentration.

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“…The physiological disorder HH (hyperhydricity) occurs frequently in tissue culture (Rojas-Martínez et al 2010) and occasionally under ex vitro conditions (Correll et al 2000;Du Chatenet et al 2000). Hyperhydric tissues are characterized by a high water content.…”

Section: Introductionmentioning

confidence: 71%

Trichloroacetate, an inhibitor of wax biosynthesis, prevents the development of hyperhydricity in Arabidopsis seedlings

Klerk¹,

Pramanik²

2017

Plant Cell Tiss Organ Cult

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Controlling hyperhydration of carnations (Dianthus caryophyllus L.) grown in a mist reactor

Cited by 26 publications

References 44 publications

Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers

Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers

Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla

Trichloroacetate, an inhibitor of wax biosynthesis, prevents the development of hyperhydricity in Arabidopsis seedlings

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