Controlling molecular transport through nanopores

Keyser, Ulrich F.

doi:10.1098/rsif.2011.0222

Cited by 173 publications

(192 citation statements)

References 113 publications

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“…1 Solid state nanopores made in insulating materials have been used for sensing a wide range of biomolecules. [2][3][4] Measurements are typically taken with a commercially available patch clamp amplifier (such as the Heka EPC 10 and Axon Axopatch 200B) measuring the ionic current across one nanopore at a time. Every nanopore experiment requires statistics on many single molecule translocations to collect meaningful data.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed ionic current sensing with glass nanopores

et al. 2013

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Section: Introductionmentioning

confidence: 99%

Multiplexed ionic current sensing with glass nanopores

et al. 2013

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“…For other secretion systems, combining (partial) in vitro reconstitution of secretion systems into artificial membranes with biophysical tools seems to be a promising approach. [104] Furthermore, a bottom-up approach generating synthetic translocation ratchets (e.g. by substituting biases in chemical potentials [5] ) would be interesting to better understand force generation by these machines.…”

Section: Resultsmentioning

confidence: 99%

Bacterial Translocation Ratchets: Shared Physical Principles with Different Molecular Implementations

Hepp

Maier

2017

BioEssays

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Secretion systems enable bacteria to import and secrete large macromolecules including DNA and proteins. While most components of these systems have been identified, the molecular mechanisms of macromolecular transport remain poorly understood. Recent findings suggest that various bacterial secretion systems make use of the translocation ratchet mechanism for transporting polymers across the cell envelope. Translocation ratchets are powered by chemical potential differences generated by concentration gradients of ions or molecules that are specific to the respective secretion systems. Bacteria employ these potential differences for biasing Brownian motion of the macromolecules within the conduits of the secretion systems. Candidates for this mechanism include DNA import by the type II secretion/ type IV pilus system, DNA export by the type IV secretion system, and protein export by the type I secretion system. Here, we propose that these three secretion systems employ different molecular implementations of the translocation ratchet mechanism.

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“…Nanopores have shown to allow linear translocations of DNA molecules in buffer conditions (Fologea et al 2005a;Heng et al 2004;Li et al 2003) when a bias voltage is applied, allowing with these devices the detection and characterization of DNA molecules, although the highspeed passage of DNA through pores with this method still remains a problem (Chen et al 2010;Fologea et al 2005b;Gierhart et al 2008;Keyser 2011;Lagerqvist et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Laser fabrication of micropores and their integration to microfluidic platforms for DNA electrophoresis

et al. 2012

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The work presents an alternative method for the manufacture of micropores by using the combination of laser ablation and wet etching. The process of laser ablation was done on silicon (Si) wafers with silicon nitride (Si 3 N 4 ) used as a sacrificial layer. The size of the pores was carefully controlled by following an optical technique. The method exhibits a series of advantages in relation micromachining techniques previously used. The fabricated pores were then integrated to polydimethylsiloxane (PDMS) microchannels, which constitutes a novel setup to be considered for microfluidic applications. Furthermore, as the system is addressed to study the electrically-driven transport of macromolecules, deoxyribonucleic acid (DNA) electrophoresis was performed in the fabricated microsystems to prove the principle. Also for these purposes, the electric field throughout the microchannel and pore region was studied in details by using numerical simulations.

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Controlling molecular transport through nanopores

Cited by 173 publications

References 113 publications

Multiplexed ionic current sensing with glass nanopores

Multiplexed ionic current sensing with glass nanopores

Bacterial Translocation Ratchets: Shared Physical Principles with Different Molecular Implementations

Laser fabrication of micropores and their integration to microfluidic platforms for DNA electrophoresis

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