Controlling of foodborne pathogen biofilms on stainless steel by bacteriophages: A systematic review and meta-analysis

Azari, Rahim; Yousefi, Mohammad Hashem; Fallah, Aziz A.; Alimohammadi, Arezoo; Nikjoo, Nastaran; Wagemans, Jeroen; Berizi, Enayat; Hosseinzadeh, Saeid; Ghasemi, Mohammad; Mousavi Khaneghah, Amin

doi:10.1016/j.bioflm.2023.100170

Cited by 3 publications

(1 citation statement)

References 189 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the hospital environment, they is capable of being developed into catheters, probes, prostheses and other biomedical devices, posing a risk to immunocompromised patients [11]. In the food industry, biofilms on stainless steel surfaces have been reported and are considered a risk to consumer food safety [12]. In this context, phages with the capacity to infect P. aeruginosa, E. coli, E. faecalis and S. aureus have already been reported [13][14][15] and are relevant for directing further studies on strategies to combat bacterial contamination and/or infections in several areas, such as human health, water treatment [1], agriculture [2], the food industry [3] and animal husbandry [4].…”

Section: Introductionmentioning

confidence: 99%

The Antibacterial Activity of Novel Bacteriophages and the Emergence of Bacterial Resistance to Phage Infections: An In Vitro Study

Moda-Silva,

Oliveira,

da Cruz

et al. 2024

Applied Biosciences

View full text Add to dashboard Cite

The emergence of bacteria resistant to bacteriophage (phage) infection may compromise the success and effectiveness of phage therapy. The aim of this study was to evaluate the in vitro antibacterial activity of five novel phages, as well as the emergence of bacterial resistance to phage infections. The antibacterial activity of lytic phages was evaluated against standard strains of Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25927), Enterococcus faecalis (ATCC 29212) and Staphylococcus aureus (ATCC 6538). Phages were initially grown in the presence of host bacteria in an exponential growth phase, then purified and titrated. In a second exposure, 20 μL of each phage was inoculated with 106 CFU/mL of P. aeruginosa/E. coli/E. faecalis/S. aureus, separately. In a third exposure, resistant colonies were isolated, cultivated and exposed again to the phages. Bacterial colonies resistant to phage infection after the third exposure were evaluated for their susceptibility profile to different antibiotics via the diffusion disk technique. The diameters of the inhibition halos were evaluated with Image J software (version 1.54g) and the definition of the susceptibility profile to antibiotics was determined according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. In addition, fourteen cocktails with different phages were formulated to evaluate the emergence of a bacterial resistance to phage infections. The phages exhibited specificity for P. aeruginosa and did not infect E. coli, E. faecalis and S. aureus. The presence of bacterial colonies resistant to phage infection in the three successive exposures was identified, and the bacterial resistance to phage infection was confirmed in all phages titrated at 108 PFU/mL, in four phages titrated at 1010 PFU/mL and in one phage titrated at 1013 PFU/mL. The development of a resistance to infection by phages (~108 PFU/mL) did not change the susceptibility profile of P. aeruginosa to antibiotics and, when evaluating the emergence of a resistance to infection by phage cocktails (~108 PFU/mL, ~1010 PFU/mL, ~1013 PFU/mL), bacterial resistance to phage infection was confirmed in all cocktails with phages titrated at 108 PFU/mL, in ten cocktails with phages titrated at 1010 PFU/mL and in seven cocktails with phages titrated at 1013 PFU/mL. In conclusion, the presence of resistant P. aeruginosa colonies to phage infection after successive exposures was evidenced, although some phages at title ~1010 PFU/mL and ~1013 PFU/mL were effective in inhibiting the growth of resistant colonies. The development of resistance did not change the susceptibility profile of P. aeruginosa to antibiotics. Variants of P. aeruginosa that were resistant to phage infection were isolated and their resistance to infection via the phage cocktail was demonstrated regardless of the viral titer, although some cocktails at title ~1010 PFU/mL and ~1013 PFU/mL were effective in inhibiting the growth of resistant colonies. Despite the emergence of bacterial variants resistant to phage infection, new studies involving the applicability of phages in the control of infections must be conducted.

show abstract

Section: Introductionmentioning

confidence: 99%

The Antibacterial Activity of Novel Bacteriophages and the Emergence of Bacterial Resistance to Phage Infections: An In Vitro Study

Moda-Silva,

Oliveira,

da Cruz

et al. 2024

Applied Biosciences

View full text Add to dashboard Cite

show abstract

Phage-based biocontrol of Porphyromonas gingivalis through indirect targeting

Wu,

Fujiki,

Mathieu

et al. 2024

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacteriophages offer an opportunity for chemical-free, precise control of problematic bacteria, but this approach can be limited when lytic phages are difficult to obtain for the target host. In such cases, phage-based targeting of cooperating or cross-feeding bacteria (e.g., Streptococcus gordonii ) can be an effective approach to control the problematic bacteria (e.g., Porphyromonas gingivalis ). Using a dual-species biofilm system, phage predation of S. gordonii (10 8 PFU·mL −1 ) decreased the abundance of pathogenic P. gingivalis by >99% compared with no-treatment controls, while also inhibiting the production of cytotoxic metabolic end products (butyric and propionic acids). Phage treatment upregulated genes associated with interspecies co-adhesion (5- to 8-fold) and quorum sensing (10-fold) in residual P. gingivalis , which is conducive to increased potential to bind to S. gordonii . Counterintuitively, lower-titer phage applications (10 4 PFU·mL −1 ) increased the production of extracellular polymeric substance (EPS) by 22% and biofilm biomass by 50%. This overproduction of EPS may contribute to the phenomenon where the biofilm separated into two distinct species layers, as observed by confocal laser scanning microscopy. Although more complex mixed-culture systems should be considered to delineate the merits and limitations of this novel biocontrol approach (which would likely require the use of phage cocktails), our results offer proof of concept that indirect phage-based targeting can expand the applicability of phage-based control of pathogenic bacteria for public health protection. IMPORTANCE Lytic phages are valuable agents for targeted elimination of bacteria in diverse applications. Nevertheless, lytic phages are difficult to isolate for some target pathogens. We offer proof of concept that this limitation may be overcome via indirect phage targeting, which involves knocking out species that interact closely with and benefit the primary problematic target bacteria. Our target ( P. gingivalis ) only forms a periodontal pathogenic biofilm if the pioneer colonizer ( S. gordonii ) offers its surface for P. gingivalis to attach. Phage predation of the co-adhesive S. gordonii significantly reduced abundance of the target pathogen by >99%, decreased the total biofilm biomass by >44%, and suppressed its production of cytotoxic metabolic byproducts. Thus, this research extends the scope of phage-based biocontrol for public health protection.

show abstract

Gıda Endüstrisinde Bakteriyel Biyofilm Oluşumu, Kontrolü ve Giderilmesine Yönelik Yeni Uygulamalar

Kayaardı,

Uyarcan,

Turan

2024

Dicle Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Gıda zincirinde hammaddenin işletmeye girmesinden son ürün elde edilinceye kadar üretimin tüm aşamalarında ekipmanların yeterince temizlenmemesi ve uygun hijyen standartlarının ihmal edilmesi gibi nedenlerle çeşitli kaynaklardan kontaminasyon riski söz konusudur. Gıda işletmelerinde yaşanan kontaminasyon riskleri sadece ürün kalitesini değil aynı zamanda gıda güvenliğini ve tüketici sağlığını etkileme potansiyeline sahiptir. Kontaminasyonu önlemeye yönelik yapılan temizlik ve dezenfeksiyon uygulamalarında yaşanan en büyük zorluklardan biri ekipman yüzeylerinde bakteriyel kaynaklı biyofilm oluşumudur. Biyofilmler bir yüzeye yapışarak kendi ürettikleri polimerik yapı içinde yaşayan mikroorganizmaların oluşturduğu jelsi bir tabaka olarak tanımlanmaktadır. Özellikle gıda işletmelerinde ekipman ve boruların iç yüzeyleri, filtreler, konveyör bantlar, yardımcı alet ve ekipmanlarda, temizlenmesi ve dezenfekte edilmesi zor olan alanlarda gelişerek gıdalarda bozulmalara neden olmakla birlikte ciddi ekonomik kayıplara yol açmaktadır. Bu yüzden gıda işletmelerinde biyofilm oluşumunun engellenmesi ya da engellenemediği durumlarda da ortadan kaldırılması gerekmektedir. Son zamanlarda güncel çalışmalar biyofilmlerin endüstriyel ortamlardan giderilmesi veya oluşumunun engellenmesi için mevcut yöntem ve stratejilerin iyileştirilmesiyle birlikte daha etkili inhibitör ajanlar veya uzaklaştırma tekniklerinin geliştirilmesine odaklanmıştır. Biyofilmlerin kontrolünde son uygulamalar arasında soğuk atmosferik plazma, yüksek basınç, vurgulu ışık, elektrolize su, ozon, ultrason gibi yeşil teknolojiler ile bakteriyofaj ve bakteriyosin uygulamaları yer almaktadır. Biyofilm engel teknolojilerine bakıldığında bakterisidal yüzey teknolojileri ve nanoteknoloji gibi yeni teknikler üzerine güncel çalışmalar devam etmektedir. Bu çalışmada bakteriyel biyofilm oluşumu ve gelişimi, etkileyen faktörler, önlemeye yönelik stratejiler ile her şeye rağmen oluşumu engellenemeyen biyofilmlerin gıda güvenliği, tüketici sağlığı, ekipman ve yüzeylere zarar vermeyecek ileri tekniklerle en etkili şekilde giderilmesine yönelik uygulamalar derlenmiştir.

show abstract

Controlling of foodborne pathogen biofilms on stainless steel by bacteriophages: A systematic review and meta-analysis

Cited by 3 publications

References 189 publications

The Antibacterial Activity of Novel Bacteriophages and the Emergence of Bacterial Resistance to Phage Infections: An In Vitro Study

The Antibacterial Activity of Novel Bacteriophages and the Emergence of Bacterial Resistance to Phage Infections: An In Vitro Study

Phage-based biocontrol of Porphyromonas gingivalis through indirect targeting

Gıda Endüstrisinde Bakteriyel Biyofilm Oluşumu, Kontrolü ve Giderilmesine Yönelik Yeni Uygulamalar

Contact Info

Product

Resources

About