Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Annabi, Nasim; Nichol, Jason W.; Zhong, Xia; Ji, Chao; Koshy, Sandeep T.; Khademhosseini, Ali; Dehghani, Fariba

doi:10.1089/ten.teb.2009.0639

Cited by 1,035 publications

(845 citation statements)

References 108 publications

Supporting

Mentioning

818

Contrasting

Unclassified

Order By: Relevance

“…The quantitative determination of the pore size showed that the mean pore diameter of the scaffolds was 82 ± 7 lm and 115 ± 9 lm for PEC-US and PEC, respectively. These values are in the range of 50 to 300 lm as reported in the literature for other freeze-dried matrices [37,38]. On the other hand, the pore size distribution (Fig.…”

Section: Scaffolds Characterizationsupporting

confidence: 80%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

a b s t r a c tIn recent years, there has been an increasing interest in the design of biomaterials for cartilage tissue engineering. This type of materials must meet several requirements. In this study, we apply ultrasound to prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose (CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the interpolymer macroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemical degradation of each component in order to optimize the experimental conditions for PEC compatibilization. Scaffolds obtained applying this methodology and scaffolds without ultrasound processing were prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (by FTIR), stability and mechanical properties were analyzed. The swelling kinetics was studied and interpreted based on the structural differences between the two kinds of scaffolds. In addition we evaluate the possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstrate that the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improved properties compared with the simple mixture of the two polysaccharides. The test with murine macrophage RAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtained under ultrasound conditions could be useful in the cartilage tissue engineering field.

show abstract

Section: Scaffolds Characterizationsupporting

confidence: 80%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

show abstract

“…Ionically crosslinked samples fragmented significantly after 1 month in vivo, resulting in cell infiltration, whereas the click alginate hydrogels remained intact during the 2 month study and were highly resistant to cell infiltration. In tissue engineering applications where cell trafficking within the hydrogel is desirable, click alginate hydrogels could be processed using existing techniques to introduce microscale porosity to the hydrogels [50,51]. Alternatively, click alginate polymers could be crosslinked using tetrazine or norbornene-modified matrix metalloproteinase-degradable peptide sequences to allow cell-mediated degradation [29,52].…”

Section: Discussionmentioning

confidence: 99%

Versatile click alginate hydrogels crosslinked via tetrazine–norbornene chemistry

et al. 2015

View full text Add to dashboard Cite

Abstract:Alginate hydrogels are well-characterized, biologically inert materials that are used in many biomedical applications for the delivery of drugs, proteins, and cells. Unfortunately, canonical covalently crosslinked alginate hydrogels are formed using chemical strategies that can be biologically harmful due to their lack of chemoselectivity. In this work we introduce tetrazine and norbornene groups to alginate polymer chains and subsequently form covalently crosslinked click alginate hydrogels capable of encapsulating cells without damaging them. The rapid, bioorthogonal, and specific click reaction is irreversible and allows for easy incorporation of cells with high post-encapsulation viability. The swelling and mechanical properties of the click alginate hydrogel can be tuned via the total polymer concentration and the stoichiometric ratio of the complementary click functional groups. The click alginate hydrogel can be modified after gelation to display cell adhesion peptides for 2D cell culture using thiol-ene chemistry.Furthermore, click alginate hydrogels are minimally inflammatory, maintain structural integrity over several months, and reject cell infiltration when injected subcutaneously in mice. Click alginate hydrogels combine the numerous benefits of alginate hydrogels with powerful bioorthogonal click chemistry for use in tissue engineering applications involving the stable encapsulation or delivery of cells or bioactive molecules.

show abstract

“…[ 28 ] Such patterned architectures are highly desirable for tissue engineering applications; for example, anisotropic gel environments are used to guide cellular alignment and microchannel architectures are exploited for enhanced nutrient mass transport. [ 29 ] Conversely, under the same experimental conditions, unidirectional calcium diffusion resulted in alginate gels with an isotropic mesh structure ( Figure S8, Supporting Information), which suggests that F127 acts as an interfacial stabilizer during the formation of both large pores and aligned channels. [ 30 ] Rheology and compression testing revealed that the hybrid gel, before and after calcium crosslinking, had physical properties signifi cantly different to the equivalent neat alginate and F127 systems.…”

Section: Doi: 101002/adhm201600022mentioning

confidence: 95%

3D Bioprinting Using a Templated Porous Bioink

Armstrong

Burke

Carter

et al. 2016

Adv Healthcare Materials

163

111

View full text Add to dashboard Cite

3D cell printing (bioprinting) is rapidly emerging as a key biofabrication strategy for engineering tissue constructs with physiological form and complexity. [1][2][3][4] In practice, this process involves layer-by-layer deposition of a cell-laden bioink resulting in the additive manufacture of a patterned architecture with different cell types, growth factors, or mechanical cues, which are positioned with far greater precision than can be achieved with conventional scaffold-based tissue engineering. [ 5 ] While there have been signifi cant advances in printing technology, [ 6,7 ] progress has been limited by the rate of development of bioinks that are compatible with both 3D printing and tissue engineering. [ 8 ] These materials must be able to withstand extrusion, maintain structural fi delity for long time periods, and permit adequate nutrient diffusion, all under cytocompatible conditions. Due to their intrinsic porosity and capacity for high nutrient loading, hydrogels are the most promising candidate for bioink design, [ 9 ] particularly when gelation can be externally triggered using chemical bonding, [ 10 ] photoinduced crosslinking, [ 11 ] thermal setting, [ 12 ] or shear-thinning. [ 13 ] However, integrating these factors into a system while maintaining printability, structural persistence, and cell viability, is an enduring challenge. [ 14 ] Pluronic block copolymers of poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) present a possible pathway to print gelation, as they undergo a sol-gel transition upon heating near physiological temperatures. Here, elevating the temperature of these non-ionic surfactants reduces the

show abstract

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Cited by 1,035 publications

References 108 publications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Versatile click alginate hydrogels crosslinked via tetrazine–norbornene chemistry

3D Bioprinting Using a Templated Porous Bioink

Contact Info

Product

Resources

About