Controlling the rheology of gellan gum hydrogels in cell culture conditions

Moxon, Samuel R.; Smith, Alan M.

doi:10.1016/j.ijbiomac.2015.12.007

Cited by 31 publications

(18 citation statements)

References 34 publications

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“…GG solution was poured onto 48-well plate (1 mL/well); refrigerated at 4 °C for 4 h; and frozen at −20 °C for 4 h and −75 °C for 24 h. The frozen gels were lyophilized for 48 h. In this process, the gelation mechanism occurs after the coil to helix transition. [7,22] The GG sponges may be physically cross-linked due to temperature variation.…”

Section: Fabrication Of Gellan Gum Spongesmentioning

confidence: 99%

Inflammatory response study of gellan gum impregnated duck’s feet derived collagen sponges

Song

Lee

Cha

et al. 2016

Journal of Biomaterials Science, Polymer Edition

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Tissue engineered biomaterials have biodegradable and biocompatible properties. In this study, we have fabricated sponges using duck's feet derived collagen (DC) and gellan gum (GG), and further studied its inflammatory responses. The as-prepared duck's feet DC/GG sponges showed the possibility of application as a tissue engineering material through in vitro and in vivo experiments. The physical and chemical properties of sponges were characterized by compression strength, porosity, and scanning electron microscopy, etc. In vitro cell viability were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. An inflammatory response was studied after seeding RAW264.7 cells on as-fabricated sponges using reverse transcriptase-polymerase chain reaction. In vivo studies were carried out by implanting in subcutaneous nude mouse followed by extraction, histological staining. Collectively, superior results were showed by DC/GG sponges than GG sponge in terms of physical property and cell proliferation and thus can be considered as a potential candidate for future tissue engineering applications.

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Section: Fabrication Of Gellan Gum Spongesmentioning

confidence: 99%

Inflammatory response study of gellan gum impregnated duck’s feet derived collagen sponges

Song

Lee

Cha

et al. 2016

Journal of Biomaterials Science, Polymer Edition

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“…In recent years, various types of materials have been used as three-dimensional (3D) matrices, such as chitosan-alginate scaffolds, collagen, alginate and agarose ( 7 – 10 ). These biomaterials are nontoxic and can alter the rheology properties of the culture system ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

A novel three‑dimensional tumorsphere culture system for the efficient and low‑cost enrichment of cancer stem cells with natural polymers

et al. 2017

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Cancer stem cells (CSCs) are considered to serve a key role in tumor progression, recurrence and metastasis. Tumorsphere culture is the most important method for enriching CSCs and is widely used in basic research and drug screening. However, the traditional suspension cell culture system has several disadvantages, including low efficiency, high cost and difficult procedure, making it difficult to produce tumorspheres on a large scale. In the present study, two biomaterials, methylcellulose (MC) and gellan gum (GG), were used to construct a novel culture system based on the traditional system. Subsequently, the characteristics of the novel three-dimensional (3D) culture system were evaluated, the design scheme was optimized, and the morphological and biological features of the tumorspheres cultured in this 3D system were compared with the traditional system. The results revealed that the tumorspheres cultured in the novel 3D system presented a higher seeding density and improved morphology, while maintaining stem-like properties. This evidence suggests that a simple, efficient and low-cost culture system that produces tumorspheres on a large scale was successfully constructed, which can be widely used in various aspects of stem cell research.

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“…Gellam gum is a bacterial polysaccharide produced by Sphingomonas elodea and has potential industrial applications as a gelling agent alternate to agarose (agar) and carrageenans in the food industry, medicine and biotechnology [39]- [42]. The primary structure of the polysaccharide consists of tetrasaccharide repeating units, [44].…”

Section: Introductionmentioning

confidence: 99%

Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

Tako¹,

Kitajima²,

Yogi³

et al. 2016

ABC

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The structure-function relationship of a gellan family of polysaccharides, S-198 gum produced by Alcaligenes ATCC31853 was investigated in terms of rheological aspects. The flow curves of S-198 gum showed plastic behavior above 0.3%. Gelation did not occur in S-198 gum solution at low temperature (0˚C), even at 0.8%. Both the viscosity and the elastic modulus remained constant with increasing temperature up to 80˚C. The elastic modulus decreased a little with the addition of CaCl2 (6.8 mM), but then once again remained constant up to 80˚C. The highest elastic modulus was observed for deacylated gellan gum with the addition of CaCl 2 and increased slightly with increasing temperature up to 80˚C, which was considered to be a transition temperature, after which it decreased rapidly. The elastic modulus of S-198 gum in the presence of urea (4.0 M) was lower than that in aqueous solution at low temperature (0˚C), but remained constant with increasing temperature up to 80˚C. The intramolecular associations, (hydrogen bonding and van der Waals forces of attraction), of S-198 gum molecules in aqueous solutions were proposed. The gellan family of polysaccharides, S-198, S-88, S-657, rhamsan, welan and gellan gum, provided a good opportunity to investigate the structure-function relationship for polysaccharides.

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Controlling the rheology of gellan gum hydrogels in cell culture conditions

Cited by 31 publications

References 34 publications

Inflammatory response study of gellan gum impregnated duck’s feet derived collagen sponges

Inflammatory response study of gellan gum impregnated duck’s feet derived collagen sponges

A novel three‑dimensional tumorsphere culture system for the efficient and low‑cost enrichment of cancer stem cells with natural polymers

Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

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