Controlling vegetative cells and spores growth of Bacillus spp. using ethanolic Ketapang (Terminalia catappa L.) leaf extract

Madhavan, Kierrthanah; Rukayadi, Yaya; Abdul-Mutalib, Noor Azira

doi:10.1016/j.heliyon.2023.e18749

Cited by 5 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…50 Overally, the trend observed for the these assays was time-dependent. 29 Proximate Composition Analysis and Pearson's Correlation. The proximate analysis of the potato and market wastes as plant-based substrates and pig and rabbit manures as animal-derived feeds forH.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The longer time taken by HIL extracts from animal-based substrates to destroy the bacterial population might be a result of lower AMP expression in these extracts . Overally, the trend observed for the these assays was time-dependent …”

Section: Resultsmentioning

confidence: 99%

“…A time-kill assay was performed on 20% AcOH HIL extracts against four test microorganisms according to the previously described method with slight modifications . In summary, the bacterial culture suspension was adjusted to approximately 1.0 × 10 6 CFU/mL.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…A time-kill assay was performed on 20% AcOH HIL extracts against four test microorganisms according to the previously described method with slight modifications. 29 In summary, the bacterial culture suspension was adjusted to approximately 1.0 × 10 6 CFU/mL. The HIL extracts were diluted with MHB medium containing the inoculum to obtain final concentrations of 0 × MIC (0 mg/mL) for control and 1 × MIC (25 mg/mL) for HIL extracts from pig manure, rabbit manure, potato waste, and market waste.…”

Section: Materials and Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Unlocking the Potential of Substrate Quality for the Enhanced Antibacterial Activity of Black Soldier Fly against Pathogens

Achuoth,

Mudalungu,

Ochieng

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Globally, antibiotics are facing fierce resistance from multidrug-resistant bacterial strains. There is an urgent need for ecofriendly alternatives. Though insects are important targets for antimicrobial peptides, it has received limited research attention. This study investigated the impact of waste substrates on the production of antibacterial agents in black soldier fly (Hermetia illucens L.) larvae (HIL) and their implications in the suppression of pathogens [Bacillus subtilis (ATCC 6051), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922)]. The 20% acetic acid (AcOH) extract from market waste had the highest antibacterial activity with an inhibition zone of 17.00 mm, followed by potato waste (15.02 mm) against S. aureus. Hexane extract from HIL raised on market waste also showed a significant inhibitory zone (13.06 mm) against B. subtilis. .Minimum inhibitory concentration (MIC) values recorded were 25 mg/mL against all test pathogens. The fastest time-kill of 20% AcOH extract was 4 h againstB. subtilis, E. coli, ,and P. aeruginosa. Lauric acid was also identified as the dominant component of the various hexane extracts with concentrations of 602.76 and 318.17 μg/g in HIL reared on potato and market waste, respectively. Energy from the market waste substrate correlated significantly (r = 0.97) with antibacterial activities. This study highlights the key role of substrate quality and extraction methods for enhancing the production of antibacterial agents in HIL, thus providing new insights into the development of potential drugs to overcome the alarming concerns of antimicrobial resistance.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations