Controlling volatile fatty acids production from waste activated sludge by an alginate-degrading consortium

Hu, Zhi-Yi; Wang, Shuai; Geng, Zi-Qian; Keino, Dai; Ji, Wen-Xiang; Tian, Ye-Chao; Li, Wentao; Zeng, Raymond J.; Zhang, Fang

doi:10.1016/j.scitotenv.2021.150730

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…By the end of 2019, it is estimated that the production of sludge (80% water content) in China is up to 39 million tons . There is a strong drive to minimize this amount, and anaerobic digestion of WAS is greatly desired. ,, Felz et al recently reported that the contents of neutral sugars and uronic acids in EPS were comparable (10.7 and 13.2%) . Several researchers reported that uronic acids containing polymers in EPS are resistant to anaerobic degradation. , In this work, we demonstrate that Bacteroides -dominated ADC with the trait to excrete alginate lyases (EC 4.2.2.3) can enhance EPS conversion in WAS.…”

Section: Resultssupporting

confidence: 51%

“…42 There is a strong drive to minimize this amount, and anaerobic digestion of WAS is greatly desired. 2,17,43 Felz et al recently reported that the contents of neutral sugars and uronic acids in EPS were comparable (10.7 and 13.2%). 10 Several researchers reported that uronic acids containing polymers in EPS are resistant to anaerobic degradation.…”

Section: Extracellular Alginate Lyase Identification By Metaproteomic...mentioning

confidence: 94%

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Identification of Extracellular Key Enzyme and Intracellular Metabolic Pathway in Alginate-Degrading Consortia via an Integrated Metaproteomic/Metagenomic Analysis

Geng

Qian

et al. 2021

Environ. Sci. Technol.

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Uronic acid in extracellular polymeric substances is a primary but often ignored factor related to the difficult hydrolysis of waste-activated sludge (WAS), with alginate as a typical polymer. Previously, we enriched alginate-degrading consortia (ADC) in batch reactors that can enhance methane production from WAS, but the enzymes and metabolic pathway are not well documented. In this work, two chemostats in series were operated to enrich ADC, in which 10 g/L alginate was wholly consumed. Based on it, the extracellular alginate lyase (∼130 kD, EC 4.2.2.3) in the cultures was identified by metaproteomic analysis. This enzyme offers a high specificity to convert alginate to disaccharides over other mentioned hydrolases. Genus Bacteroides (>60%) was revealed as the key bacterium for alginate conversion. A new Entner−Doudoroff pathway of alginate via 5-dehydro-4-deoxy-D-glucuronate (DDG) and 3deoxy-D-glycerol-2,5-hexdiulosonate (DGH) as the intermediates to 2-keto-3-deoxy-gluconate (KDG) was constructed based on the metagenomic and metaproteomic analysis. In summary, this work documented the core enzymes and metabolic pathway for alginate degradation, which provides a good paradigm when analyzing the degrading mechanism of unacquainted substrates. The outcome will further contribute to the application of Bacteroides-dominated ADC on WAS methanogenesis in the future.

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Section: Resultssupporting

confidence: 51%

Section: Extracellular Alginate Lyase Identification By Metaproteomic...mentioning

confidence: 94%

Identification of Extracellular Key Enzyme and Intracellular Metabolic Pathway in Alginate-Degrading Consortia via an Integrated Metaproteomic/Metagenomic Analysis

Geng

Qian

et al. 2021

Environ. Sci. Technol.

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“…The top 20 enriched bacteria at the genus level are summarized in Figure C. Zoogloea (2.1%) and Nitrospira (3.3%) in the WAS were typical EPS-producing bacteria that form activated sludge flocs in WWTPs. , After alginate was added to enrich the PCAN, the main bacteria in PCAN changed to the Bacteroides genus, with the percentage even increasing to 69.6% in PCAN4. The Pacbio sequencing presented in Figure D further demonstrated that B.…”

Section: Resultsmentioning

confidence: 99%

“…21,22 A mesophilic alginate-degrading consortium has been enriched to improve the production of methane (up to 185%) and VFA (∼35%) from WAS. 23,24 However, to the best of our knowledge, until now, most reports on alginate degradation focus on marine pure-culture bacteria under mesophilic or thermophilic conditions for brown macroalgae utilization. 25,26 Most denitrifiers work efficiently at 25−35 °C and under neutral pH conditions, 27,28 while nitrate removal has been seldom reported under psychrophilic conditions.…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Wang,

Tang,

Yan

et al. 2024

ACS EST Water

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Wastewater treatment plants (WWTPs) are being challenged to operate as carbon-neutral and energy-efficient systems. The structural extracellular polymeric substances (St-EPS) in waste-activated sludge (WAS), such as alginate, were revealed to form an architectural gel matrix, hindering the psychrophilic hydrolysis and acidogenesis and its application in nitrate removal. Consequently, volatile fatty acid (VFAs) production for nitrate removal at 15 °C is demonstrated by a novel, enriched psychrophilic consortium for alginate-degrading and nitrate-removing. Both alginate lyase (EC 4.2.2.3) and oligoalginate lyase (EC 4.2.2.26) are identified for alginate utilization by the metaproteomics analysis. A high nitrate removal rate of 7.6 (WAS as the substrate) to 13.0 (alginate as the substrate) mgN/(gVSS h) was achieved. By combining high-throughput and PacBio sequencing and metagenomics analysis, two genera of Bacteroides (69.4%) and Dysgonomons (12.8%) are revealed to enhance psychrophilic WAS fermentation. The VFAs can be utilized by denitrifiers for nitrate removal in an indirect pathway. Moreover, other genera of denitrifiers (Pseudomonas, 0.11%; and Massilla, 0.003%) can excrete alginate lyase, indicating the dual merits of St-EPS degradation and nitrate removal in the direct pathway. This work extends the application of WAS for denitrifiers, providing a new carbon-neutral and energy-efficient system in WWTPs, especially under psychrophilic conditions.

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“…The biological method is considered an important biotechnology, but the low hydrolytic rates of EPS obtained by dosing anaerobes impede WAS fermentation. The enriched uronic acids degradation bacteria, such as polygalacturonate-degrading consortium (GDC, mainly Clostridium genus (17.1%)), demonstrated the expected performance of enhancing methane (115–185%) and VFA (∼35%) production from WAS. , Apart from uronic acid in EPS, cationic hexosamine polymers were generally considered as the structural components of microbial EPS . For instance, sialic acids and GAGs comprised approximately 2% of anammox EPS and 5.5% of the collected biofilm EPS .…”

Section: Resultsmentioning

confidence: 99%

Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

Wang,

Zhu,

Hong

et al. 2023

Environ. Sci. Technol.

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Polysaccharides in extracellular polymeric substances (EPS) can form a hybrid matrix network with proteins, impeding waste-activated sludge (WAS) fermentation. Amino sugars, such as N-acetyl-D-glucosamine (GlcNAc) polymers and sialic acid, are the non-negligible components in the EPS of aerobic granules or biofilm. However, the occurrence of amino sugars in WAS and their degradation remains unclear. Thus, amino sugars (∼6.0%) in WAS were revealed, and the genera of Lactococcus and Zoogloea were identified for the first time. Chitin was used as the substrate to enrich a chitin-degrading consortium (CDC). The COD balances for methane production ranged from 83.3 and 95.1%. Chitin was gradually converted to oligosaccharides and GlcNAc after dosing with the extracellular enzyme. After doing enriched CDC in WAS, the final methane production markedly increased to 60.4 ± 0.6 mL, reflecting an increase of ∼62%. Four model substrates of amino sugars (GlcNAc and sialic acid) and polysaccharides (cellulose and dextran) could be used by CDC. Treponema (34.3%) was identified as the core bacterium via excreting chitinases (EC 3.2.1.14) and N-acetyl-glucosaminidases (EC 3.2.1.52), especially the genetic abundance of chitinases in CDC was 2.5 times higher than that of WAS. Thus, this study provides an elegant method for the utilization of amino sugar-enriched organics.

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Controlling volatile fatty acids production from waste activated sludge by an alginate-degrading consortium

Cited by 15 publications

References 38 publications

Identification of Extracellular Key Enzyme and Intracellular Metabolic Pathway in Alginate-Degrading Consortia via an Integrated Metaproteomic/Metagenomic Analysis

Identification of Extracellular Key Enzyme and Intracellular Metabolic Pathway in Alginate-Degrading Consortia via an Integrated Metaproteomic/Metagenomic Analysis

Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

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