Contusion of Skeletal Muscle Increases Leukocyte-Endothelial Cell Interactions

Menth-Chiari, Wolfgang A.; Curl, Walton W.; Rosencrance, Eileen; Smith, Thomas L.

doi:10.1097/00005373-199810000-00014

Cited by 14 publications

(14 citation statements)

References 25 publications

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“…We concluded that a 3-cm defect is not a consistent nonunion model and that cmpty defects longer than 3 cm without treatment would probably result in a 100% nonunion rate. This is corroborated by the finding of Hertel et al that a 7-cm defect did not show a spontaneous healing response (4).…”

Section: Replysupporting

confidence: 63%

“…The animal model described by Hertel et al (4) consists of a segmental tibial defect in sheep, stabilized with a custom-made A 0 unreamed interlocking tibial nail. Their model is similar to ours except that we used thrcc instead of two interlocking bolts proximal and distal to the defect.…”

Section: Replymentioning

confidence: 99%

“…However, there are some substantial differences between our study and the previous models as quoted by Dr. Menth-Chiari, which need to be addressed. As Dr. Menth-Chiari correctly emphasizes, nearly all existing models of closed soft-tissue trauma allowing for intravital microscopic studies on skeletal muscle microcirculation focus on low-energy trauma, mostly induced by the drop-mass technique on the dorsal skinfold chamber (4,7,8). However.…”

Section: Replymentioning

confidence: 99%

“…However, in view of the previously mentioned substantial differences between both models, we do not share the opinion of Dr. Menth-Chiari that this study is simply a confirmation of his previous findings. The recent study by Menth-Chiari et al (4) had not yet been published by the time we had submitted the manuscript; therefore, it was not used as a reference.…”

Section: Replymentioning

confidence: 99%

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Letters to the editors

Hertel

2000

Journal Orthopaedic Research

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Section: Replysupporting

confidence: 63%

Section: Replymentioning

confidence: 99%

Section: Replymentioning

confidence: 99%

Section: Replymentioning

confidence: 99%

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Letters to the editors

Hertel

2000

Journal Orthopaedic Research

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“…Trauma-induced structural and functional damage to the local host tissue causes devascularization, malperfusion, disturbance of endothelial permeability, hypoxia, acidosis, hematoma, edema and increased intracompartmental pressure [10,11,17,39]. In vivo microscopy studies by Menth-Chiari et al (1998) and Schaser et al (1999) demonstrated that the damaging effects of closed soft tissue trauma result in a substantial and sustained impairment of nutritive perfusion, indicated by a significant reduction in functional capillary density [24,32]. This may result in an impaired humoral and cellular immune competence [15].…”

Section: Introductionmentioning

confidence: 99%

Influence of a standardized closed soft tissue trauma on resistance to local infection. An experimental study in rats

Kälicke

Schlegel

Printzen

et al. 2003

Journal Orthopaedic Research

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Purpose: The etiology of local posttraumatic infection in the locomotor system depends on the amount, virulence and pathogenicity of the inoculated microorganisms and the locallsystemic host damage due to the type and extent of the accident or iatrogenic trauma. The relative effect of these factors remains unclear. In particular, it is still unclear today whether-in presence of microorganisms-soft tissue damage and its pathophysiological consequences lead to infection after soft tissue trauma, or whether the bacterial contamination is the primarily cause for posttraumatic infection. The aim of the project was to gain information on the consequences of a soft tissue injury in terms of resistance to local infection. Since clinical populations are too heterogeneous, the problem was investigated in a standardized, reduced (no surgery or implants) experimental in vivo model.Met/zod In female Sprague-Dawley-rats with a standardized closed soft tissue trauma to the tibialis anterior muscle (group 1: n = 13) or without (group 11: n = 13), we compared the incidence of local infection after a pairwise local, percutaneously injected bacterial challenge with various concentrations of Staphylococcus aureus (2 x 104-2 x lo6 colony forming units, CFU). The standardized closed soft tissue trauma was created by application of a specially designed, computer controlled impact device. The contaminated soft tissue and the underlying bone were removed under sterile conditions after five days and quantitatively evaluated for bacterial growths. Infection was defined as positive bacterial growth at the soft tissue and/or bone. A stepwise experimental design with an "up-and-down" dosage technique was used to adjust the bacterial challenge in the area of the ID50 (50% infection dose). Statistical evaluation of the difference between the infection rates of both groups was performed by two-sided fisher exact test (p < 0.05). Results:The overall infection rate was 46%. For the group with soft tissue trauma the ID50 was 1.32 x lo5 CFU and 1.05 x lo6 CFU for the group without soft tissue trauma. The infection rate was 69% (9 of 13 animals) for the group with soft tissue trauma and 23%) (3 of 13 animals) for the group without soft tissue trauma. This difference is statistically significant 0, = 0.047).Conclusions: The infection rate after a standardized closed soft tissue injury was significantly higher and the ID50 lower than without soft tissue trauma. Our results demonstrate that in presence of microorganisms it is not primarily the bacterial contamination but rather the soft tissue damage and its pathophysiological consequences resulting in decreased infection resistance that secondarily lead to infection.

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Efficiency of small-volume resuscitation in restoration of disturbed skeletal muscle microcirculation after soft-tissue trauma and haemorrhagic shock

Gierer

Vollmar

Schaser

et al. 2004

Langenbeck's Archives of Surgery

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Resuscitation with HyperHES is as effective as HES in improving capillary perfusion in traumatised skeletal muscle during haemorrhagic shock. However, because values of functional capillary density in the HyperHES-treated and HES-treated animals were still markedly below those reported in traumatised skeletal muscle of normovolaemic animals, further tools are needed to enhance efficiency in treatment of local skeletal muscle tissue injury during haemorrhagic shock.

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Contusion of Skeletal Muscle Increases Leukocyte-Endothelial Cell Interactions

Abstract: In the mid-term to long-term stages of skeletal muscle injury associated with contusion, a significant portion of tissue damage is secondary to leukocyte-endothelial cell interactions.

Cited by 14 publications

References 25 publications

Letters to the editors

Letters to the editors

Influence of a standardized closed soft tissue trauma on resistance to local infection. An experimental study in rats

Efficiency of small-volume resuscitation in restoration of disturbed skeletal muscle microcirculation after soft-tissue trauma and haemorrhagic shock

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