Convection‐enhanced mass transfer in aggregated beads for gel chromatography

Li, Qiangliang; Grandmaison, E. W.; Goosen, Mattheus F. A.; Taylor, David G.

doi:10.1002/aic.690461005

Cited by 5 publications

(2 citation statements)

References 42 publications

(44 reference statements)

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“…The theoretical concept and experimental utilization of an intraparticle-forced convection in classical solid-liquid chromatography for reducing mobile-phase mass transfer resistance originating in the (intraparticle) stagnant zone of packed beds has received a significant attention over the last decade [1][2][3][4][5][6][7][8][9][10][11][12][13][14] although consequences of this phenomenon were recognized already earlier, e.g., in size-exclusion chromatography [15] and catalyst design [16,17], or for the nutrient transport in biological pellets [18]. By using porous particles with a tailored hydraulic permeability such that the pressure drops typically encountered in packed columns act as a decent driving force for an intraparticle flow [8,19] the non-zero velocity component can assist (or even dominate -depending on its magnitude relative to time scales of analyte diffusion and adsorption-desorption processes) conventional, i.e., diffusion-limited intraparticle transport.…”

Section: Introductionmentioning

confidence: 99%

Perfusive flow and intraparticle distribution of a neutral analyte in capillary electrochromatography

Tallarek¹,

Pačes²,

Rapp³

2003

Electrophoresis

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The relevance and magnitude of an electroosmotic perfusion mechanism in electrochromatography is analyzed. To systemize our studies we first considered the transport of an electroneutral and nonadsorbing tracer. Based on the refractive index matching in a microfluidic setup containing fixed spherical porous particles, we conducted a quantitative analysis in real time of the spatio-temporal distribution of fluorescent tracer molecules during their uptake by (and a release from) single particles using confocal laser scanning microscopy. Even under conditions of a significant electrical double layer overlap the intraparticle electroosmotic flow produces due to its unidirectional nature and in striking contrast to the symmetric (spherical) distributions typical for purely diffusive transport strongly asymmetric concentration profiles inside spherical particles as the locally charged pore liquid begins to respond to the externally applied electrical field. The profiles retain an axisymmetric nature, i.e., rotational symmetry with respect to the field direction. Results of our measurements could be successfully interpreted and further analyzed by a compact mathematical model. Intraparticle Peclet numbers of up to 150 have been realized and found to significantly enhance the mass transport on particle scale towards the convection-dominated regime when compared to a conventional (diffusion-limited) kinetics.

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Section: Introductionmentioning

confidence: 99%

Perfusive flow and intraparticle distribution of a neutral analyte in capillary electrochromatography

Tallarek¹,

Pačes²,

Rapp³

2003

Electrophoresis

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“…Microencapsulation systems have found applications in encapsulated cell therapy/tissue engineering [1][2][3] , bioseparations technology 4 , immobilized biocatalysts 5,6 , and polymeric drug-delivery systems 7,8 . All areas, however, suffer from specific mass transfer problems.…”

Section: Introductionmentioning

confidence: 99%

Experimental and modeling studies of mass transfer in microencapsulated cell systems

Goosen

2002

Trop. J. Pharm Res

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Gaining a better understanding of mass transfer problems in encapsulated cell systems and in tissue engineering requires both experimental investigations and mathematical modelling. Specific mass transfer studies are reviewed including oxygen transfer in immobilised animal cell culture systems, modelling of electrostatic polymer droplet formation, and growth of plant somatic tissue encapsulated in alginate using electrostatics.

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Adsorption Kinetics

2010

Protein Chromatography

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Convection‐enhanced mass transfer in aggregated beads for gel chromatography

Abstract: Con®ection-enhanced mass transfer in aggregated -

Cited by 5 publications

References 42 publications

Perfusive flow and intraparticle distribution of a neutral analyte in capillary electrochromatography

Perfusive flow and intraparticle distribution of a neutral analyte in capillary electrochromatography

Experimental and modeling studies of mass transfer in microencapsulated cell systems

Adsorption Kinetics

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