Twisted tape insert was applied as a swirling flow generator for the passive heat transfer enhancement in the present work. The influences of the perforated twisted tapes (PTs) on the heat transfer, pressure loss and thermal performance characteristics were investigated experimentally. The experiments were performed under uniform wall heat flux condition by using PTs with y/W = 3, 4 and 5, d/W = 0.11, 0.14 and 0.17 and s/W = 0.4, 0.6 and 0.8 where y is a twist length, d is a perforation hole diameter, s is a spacing between holes (pitch) and W is a tape width. The experimental results reveal that Nusselt number increased with decreasing s/W and y/W and increasing d/W. For the present range, the maximum heat transfer was obtained by utilizing the tape with s/W = 0.4, d/W = 0.17 and y/W = 3, which is higher than those obtained from the plain tube with and without typical twisted tape by around 27.4 and 86.7%, respectively. In addition, the empirical correlations for Nusselt number, friction factor and thermal performance are also proposed in the present paper.