Convenient elimination of trichloroacetic acid prior to radioimmunoassay of cyclic nucleotides

Tihon, Claude; Goren, Mayer B.; Spitz, Eva; Rickenberg, H.V.

doi:10.1016/0003-2697(77)90693-5

Cited by 65 publications

(14 citation statements)

References 1 publication

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“…To the combined supernatants solid calcium carbonate was added according to the method of Tihon et al (1977) to give a pH of approx 6.0. Complete neutralization was reached after washing the supernatant three times with an equal volume of ether saturated with aqua bidest.…”

Section: Determination Of Nucleotidesmentioning

confidence: 99%

Involvement of cyclic nucleotides in locust flight muscle metabolism

Worm

1980

Comparative Biochemistry and Physiology Part C: Comparative Pha

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Abstract--1. Flight had no significant effect on the levels of c-AMP or c-GMP in the flight muscles of Locusta mioratoria.2. Injections of 0.01 or 0.1 corpus cardiacum equivalents into the abdominal cavity did not elicit any effect on cyclic nucleotide levels either.3. Injection of A23187 resulted in a decrease of the c-AMP level, but not the c-GMP level. 4. Marked increase of the c-AMP level was found to occur in the flight muscles after injection of octopamine, whereas c-GMP level was not influenced.5. It is concluded that octopamine acts on locust flight muscles through c-AMP mediated activation of glycogen phosphorylase.

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Section: Determination Of Nucleotidesmentioning

confidence: 99%

Involvement of cyclic nucleotides in locust flight muscle metabolism

Worm

1980

Comparative Biochemistry and Physiology Part C: Comparative Pha

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“…Homogenates were cen trifuged at 1,000 g for 15 min. After neutralization with excess CaCOj [6], cAMP was analyzed in 100-pl samples by radioimmunoassay (RIA) with an anti body-based RIA kit (New England Nuclear, Boston, Mass.) by the nonacetylated procedure.…”

Section: Methodsmentioning

confidence: 99%

Sex Steroid Hormones Influence the cAMP Content in Human Endometrium during the Menstrual Cycle

Pansini¹,

Bergamini²,

Bettocchi³

et al. 1984

Gynecol Obstet Invest

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The measurement of the tissue concentrations of cyclic adenosine monophosphate (cAMP) in human endometrium shows that the levels of the nucleotide vary during the menstrual cycle, being 11.4 ± (SE) 2.5 and 37.7 ± (SE) 10.1 pmol/mg protein in the proliferative and secretory phase, respectively. The individual determinations of cAMP are significantly correlated to the estradiol/progesterone blood concentration ratio: by this means we obtained a superimposable distribution with the results of histologic examination suggesting that the hormones have a direct action in determining the cAMP levels in this tissue. The relevance of these observations for the physiology of the endometrium is discussed.

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“…Determination of cAMP.-The samples were centrifuged, and the trichloroacetic acid in the supernatants was neutralized with CaCO3, as described by Tihon et al (1977). cAMP was measured by a radioimmunoassay (Steiner et al, 1969) using acetylation of the samples (Harper & Brooker, 1975;Frandsen & Krishna, 1976) and acetylated [3H]cAMP as ligand (Skomedal et al, 1977).…”

mentioning

confidence: 99%

The antitumour agent 5-(3,3-dimethyl-1-triazeno) imidazole-4-carboxamide (DTIC) inhibits rat liver cAMP phosphodiesterase and amplifies hormone effects in hepatocytes and hepatoma cells

Larsson¹,

Haffner²,

Brłnstad³

et al. 1979

Br J Cancer

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Summary.-The antitumour agent 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (DTIC) was found to inhibit competitively the low-Km cyclic AMP phosphodiesterase activity in an ammonium-sulphate-precipitable fraction of the 2,000g supernatant of rat liver. With substrate concentration at 0-25 [M, 150 was 790 /tM for DTIC and 350 tM for theophylline. DTIC at 2 mm more than doubled the cAMP response to glucagon in hepatocytes and to adrenaline in MH1Cj hepatoma cells, indicating that it also exerts its inhibitory effect on the phosphodiesterase in intact cells. The possible contribution of the phosphodiesterase inhibition to the growthinhibitory and cytotoxic effects of DTIC is discussed.

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Convenient elimination of trichloroacetic acid prior to radioimmunoassay of cyclic nucleotides

Cited by 65 publications

References 1 publication

Involvement of cyclic nucleotides in locust flight muscle metabolism

Involvement of cyclic nucleotides in locust flight muscle metabolism

Sex Steroid Hormones Influence the cAMP Content in Human Endometrium during the Menstrual Cycle

The antitumour agent 5-(3,3-dimethyl-1-triazeno) imidazole-4-carboxamide (DTIC) inhibits rat liver cAMP phosphodiesterase and amplifies hormone effects in hepatocytes and hepatoma cells

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