RESUMO -A germinação é um processo fundamental na dinâmica populacional das plantas daninhas. Sendo assim, a presente pesquisa teve como objetivo analisar a interação entre temperatura e luz sobre a germinação de uma nova variedade da planta daninha vassourinhade-botão (Borreria densiflora var. latifolia), sob condições de câmara de germinação. Foi estudada a influência de cinco temperaturas (20, 25, 30 Palavras-chave: velocidade de germinação, sementes, biologia, soja, cana-de-açúcar.ABSTRACT -Germination is a fundamental process in weed population dynamics. Thus, this research aimed to evaluate the interaction between temperature and light upon the germination of a new variety of the weed vassourinha-de-botão (Borreria densiflora var. latifolia), under germination chamber conditions. The influence of five temperatures (20, 25, 30 and 35 o C constant and alternated 20-30 o C), in presence of 12h-photoperiod and constant dark conditions upon the germination was studied, under a randomized complete-block design, with four replications. Accumulated germination percentage and germination speed were evaluated, being the latter evaluated through the germination speed index. Data were submitted to analysis of variance through F test, followed by Tukey test at probability of 0.05. Germination occurred preferentially in presence of light, with significant interaction only between temperature and presence of light. We observed, thus, the positive photoblastism of this new variety of B. densiflora. The highest germination percentage occurred under the alternated temperature 20-30