Conventional and emerging strategies for the fabrication and functionalization of PDMS-based microfluidic devices

Shakeri, Amid; Khan, Shadman; Didar, Tohid F.

doi:10.1039/d1lc00288k

Cited by 164 publications

(112 citation statements)

References 168 publications

(287 reference statements)

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“…When the first sample was loaded in the inlet of the original chip (without chemical treatment), the liquid automatically flowed to the serpentine channel due to the intrinsic hydrophilic properties of the serpentine channel created by the plasma O2 treatment before the PDMS bonding to the glass. The plasma O2 induced radical species of silanol groups (Si-OH), alcoholic hydroxyls (C-OH), and carboxylic acids (COOH) [30,31]. This rich -OH surface is critical for covalently bonding to the soda-lime glass substrate, which also contains substantially -OH groups due to its composition formed by amorphous SiO2 up to 70% [32].…”

Section: Hydrophilicity and Microfluidic Chip Performancementioning

confidence: 99%

A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers

2022

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This research proposes a low-cost and simple operation microfluidic chip to enhance the magnetic labeling efficiency of two ischemic stroke biomarkers: cellular fibronectin (c-Fn) and matrix metallopeptidase-9 (MMP9). This fully portable and pump-free microfluidic chip is operated based on capillary attractions without any external power source and battery. It uses an integrated cellulose sponge to absorb the samples. At the same time, a magnetic field is aligned to hold the target labeled by the magnetic nanoparticles (MNPs) in the pre-concentrated chamber. By using this approach, the specific targets are labeled from the beginning of the sampling process without preliminary sample purification. The proposed study enhanced the labeling efficiency from 1 hr to 15 min. The dynamic interactions occur in the serpentine channel, while the crescent formation of MNPs in the pre-concentrated chamber, acting as a magnetic filter, improves the biomarker-MNP interaction. The labeling optimization by the proposed device influences the dynamic range by optimizing the MNP ratio to fit the linear range across the clinical cutoff value. The limit of detection (LOD) of 2.8 ng/mL and 54.6 ng/mL of c-Fn measurement were achieved for undiluted and four times dilutions of MNP, respectively. While for MMP9, the LODs were 11.5 ng/mL for undiluted functionalized MNP and 132 ng/mL for four times dilutions of functionalized MNP. The results highlight the potential use of this device for clinical sample preparation and specific magnetic target labeling.When combined with a detection system could also be used as an integrated component of a point-of-care platform.

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Section: Hydrophilicity and Microfluidic Chip Performancementioning

confidence: 99%

A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers

2022

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“…Additionally, PDMS is the most commonly used material in the manufacturing of microfluidic devices, which are an important technology for the development of systems such as drug delivery, DNA sequencing, clinical diagnostics, point of care testing and chemical synthesis [ 26 ]. The used materials in these systems should be biocompatible, optically transparent and provide fast prototyping and low fabrication cost [ 27 ], features found in PDMS.…”

Section: Introductionmentioning

confidence: 99%

Properties and Applications of PDMS for Biomedical Engineering: A Review

Miranda

Souza

Sousa

et al. 2021

JFB

363

170

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Polydimethylsiloxane (PDMS) is an elastomer with excellent optical, electrical and mechanical properties, which makes it well-suited for several engineering applications. Due to its biocompatibility, PDMS is widely used for biomedical purposes. This widespread use has also led to the massification of the soft-lithography technique, introduced for facilitating the rapid prototyping of micro and nanostructures using elastomeric materials, most notably PDMS. This technique has allowed advances in microfluidic, electronic and biomedical fields. In this review, an overview of the properties of PDMS and some of its commonly used treatments, aiming at the suitability to those fields’ needs, are presented. Applications such as microchips in the biomedical field, replication of cardiovascular flow and medical implants are also reviewed.

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“…Microfluidic method can endow Janus particles with complex structure (such as three-dimensional asymmetric structure) or sophisticated morphology (such as graphic coding morphology) and the control of particle size, which greatly expands the design and application scope of Janus particles (Shepherd et al, 2006). However, the materials used in Janus particle preparation by microfluidic method are limited to polymers, which greatly restricts their application (Shakeri et al, 2021). Especially in drug delivery, the integration of a variety of materials is desired to achieve different degradation characteristics in a single carrier, so as to realize programmed, sequential or triggered release of different drugs.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Preparation of Janus Microparticles With Temperature and pH Triggered Degradation Properties

Feng

Liu

Sang

et al. 2021

Front. Bioeng. Biotechnol.

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Based on the phase separation phenomenon in micro-droplets, polymer-lipid Janus particles were prepared on a microfluidic flow focusing chip. Phase separation of droplets was caused by solvent volatilization and Janus morphology was formed under the action of interfacial tension. Because phase change from solid to liquid of the lipid hemisphere could be triggered by physiological temperature, the lipid hemisphere could be used for rapid release of drugs. While the polymer we selected was pH sensitive that the polymer hemisphere could degrade under acidic conditions, making it possible to release drugs in a specific pH environment, such as tumor tissues. Janus particles with different structures were obtained by changing the experimental conditions. To widen the application range of the particles, fatty alcohol and fatty acid-based phase change materials were also employed to prepare the particles, such as 1-tetradecanol, 1-hexadecanol and lauric acid. The melting points of these substances are higher than the physiological temperature, which can be applied in fever triggered drug release or in thermotherapy. The introduction of poly (lactic-co-glycolic acid) enabled the formation of multicompartment particles with three distinct materials. With different degradation properties of each compartment, the particles generated in this work may find applications in programmed and sequential drug release triggered by multiple stimuli.

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Conventional and emerging strategies for the fabrication and functionalization of PDMS-based microfluidic devices

Abstract: Microfluidics is an emerging and multidisciplinary field that is of great interest to manufacturers in medicine, biotechnology, and chemistry, as it provides unique tools for the development of point-of-care diagnostics,...

Cited by 164 publications

References 168 publications

A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers

A pump-free microfluidic device for fast magnetic labeling of ischemic stroke biomarkers

Properties and Applications of PDMS for Biomedical Engineering: A Review

Microfluidic Preparation of Janus Microparticles With Temperature and pH Triggered Degradation Properties

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