Convergence of Multiple MAP3Ks on MKK3 Identifies a Set of Novel Stress MAPK Modules

Colcombet, Jean; Sözen, Cécile; Hirt, Heribert

doi:10.3389/fpls.2016.01941

Cited by 35 publications

(44 citation statements)

References 32 publications

(55 reference statements)

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“…We previously formulated the hypothesis that the MKK3-MPK1/2/7/14 module functions downstream of clade-III MAP3Ks, namely MAP3K13-20 (Colcombet et al, 2016). MKK3 was indeed shown to interact in Yeast 2-hybrid (Y2H) assays with MAP3K15-20 but with none of the 8 tested clade-I and clade-II MAP3Ks (fig.…”

Section: Resultsmentioning

confidence: 99%

“…This new result comforts this model, suggesting that the late responsive signaling MAPK pathways are more general than first thought. Therefore the module could broadly regulate a second layer of events in response to environmental constraints (Colcombet et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, in the present work, we did not see any MKK3-MPK6 functional connection in the context of wounding. Nonetheless, it is possible that such connections exist in other physiological contexts in specific cell types or organs (Colcombet et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis MPK1 and MPK2, together with MPK7 and MPK14, define the clade-C MAPKs, which were shown to function downstream of the atypical MAP2K MKK3 (Colcombet et al, 2016). The MKK3-MPK7 module was for example reported to play a role during plant–pathogen interactions as well as during drought perception; in particular, MPK1 is activated by ROS produced during the interaction with pathogenic bacteria and the drought-induced phytohormone abscisic acid (ABA) (Danquah et al, 2015; Dóczi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

Sözen

Schenk

Boudsocq

et al. 2019

Preprint

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AbstractWounding is caused by abiotic and biotic factors and triggers complex short- and long-term responses at the local and systemic level. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of MKK4/5-MPK3/6 by wounding is independent of jasmonic acid (JA) signaling and that, contrary to what happens in tobacco, this fast module does not control wound-triggered JA accumulation in Arabidopsis. We also demonstrate that a second MAPK module, constituted by MKK3 and the clade-C MAPKs MPK1/2/7, is activated by wounding in an independent manner. We provide evidence that the activation of this MKK3-MPK1/2/7 module occurs mainly through wound-induced JA production via the transcriptional regulation of upstream clade-III MAP3Ks and particularly MAP3K14. We show that mkk3 mutant plants are more susceptible to the larvae of the generalist lepidopteran herbivore Spodoptera littoralis, indicating that the MKK3-MPK1/2/7 module is involved in counteracting insect feeding.One sentence summaryWounding induces the parallel activation of a rapid signaling module (MKK4/5-MPK3/6) and a JA-dependent slow one (MAP3K14-MKK3-MPK1/2/7/14) to restrict insect feeding.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

Sözen

Schenk

Boudsocq

et al. 2019

Preprint

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“…al., 2012). Examples of PKs involved in ROS signaling include orthologs to the MAP kinase (MPK) 1 that is activated by ROS like H2O2 (Colcombet et. al., 2016) and MPK8 which helps to maintain ROS homeostasis (Takahashi et.…”

Section: Response To Stimulus and Defense Responsementioning

confidence: 99%

Comprehensive Proteomic Profiling of the Brassica carinata and Triticale Mature Stigma and Pollen

Robinson¹

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Plant sexual reproduction commences with the interaction of the mature stigma (the receptive portion of the female reproductive organ) and the mature pollen (the male gametophyte). Proteins present at their mature developmental stages are therefore likely to reflect their upcoming reproductive roles. In this study, gel-free shotgun proteomics was used to investigate the proteomes of the mature stigma and pollen grain in two species that are highly related to Canada's most important crops. We identified 7703 and 5608 Brassica carinata stigma and pollen proteins, and 11533 and 2977 triticale stigma and pollen proteins, respectively. These represent the largest Brassicaceae and Triticeae pollen and stigma proteome datasets to date. In addition, this is the first comparative analysis of developing stigmas, and it was performed using isobaric tag for relative and absolute quantification (iTRAQ). There were 251 B. carinata proteins found to be differentially abundant during stigma maturation with 154 proteins that had increased abundance, while 97 decreased in abundance. For triticale, 647 differentially abundant stigma proteins were identified with 305 and 342 proteins increasing and decreasing in abundance respectively. Chapter 1 General IntroductionFlowering plants, formally classified as Magnoliophyta, but informally known as angiosperms, are the largest division within the plant kingdom. Angiosperms are divided into two main classifications; Monocots and Dicots. One of the main differences between the two is their floral structure. Flowers are important for sexual reproduction as they contain the reproductive organs of the plant (Mauseth, 1998). Many monocots and dicots are monoclinous, containing both the female (pistil) and male (stamen) reproductive organs in the same flower (Vines, 1894). Reproduction begins when pollen, released from the stamen, interacts with the stigma of the pistil, and therefore, uncovering molecular factors involved in pollen-stigma interactions is paramount to the understanding of plant reproduction. Since proteins are the mediators of physiological function within an organism, uncovering the proteome (the entire protein complement of a cell, tissue or organism) of the mature stigma and mature pollen grain, as well as the proteins that change in abundance as the stigma matures, should contribute to unraveling the molecular processes involved in pollen-stigma interactions.In this study, gel-free proteomics was used in order to elucidate key proteins underlying molecular mechanisms regulating pollen and stigma interactions in the monocot triticale and dicot Brassica carinata. As representatives of Canada's most cultivated crops, acquiring a greater understanding of the molecular processes involved in their reproduction will aid in the development of innovative approaches to managing crop reproduction.

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Elicitors: Role in Secondary Metabolite Production in Medicinal Plants

Bharti,

Singh

et al. 2023

Interdisciplinary Biotechnological Advances

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Convergence of Multiple MAP3Ks on MKK3 Identifies a Set of Novel Stress MAPK Modules

Cited by 35 publications

References 32 publications

Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

Comprehensive Proteomic Profiling of the Brassica carinata and Triticale Mature Stigma and Pollen

Elicitors: Role in Secondary Metabolite Production in Medicinal Plants

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