2014
DOI: 10.1074/jbc.m114.610444
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Conversion of a Chaperonin GroEL-independent Protein into an Obligate Substrate

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Cited by 17 publications
(20 citation statements)
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“…Our study suggests, however, that no large systematic differences exist between the sequence features of proteins that originated from clients of the GroE system and those that are not dependent on the GroE system. This observation is consistent with the report [ 31 ] that single amino acid changes are sufficient to convert a GroE-independent protein into a dependent one. Furthermore, in a recent study [ 32 ] we studied the GroEL dependence of GFP, a eukaryotic protein that is often used as a fluorescent marker also in prokaryotic systems and folds in a GroE-dependent manner.…”
Section: Discussionsupporting
confidence: 94%
“…Our study suggests, however, that no large systematic differences exist between the sequence features of proteins that originated from clients of the GroE system and those that are not dependent on the GroE system. This observation is consistent with the report [ 31 ] that single amino acid changes are sufficient to convert a GroE-independent protein into a dependent one. Furthermore, in a recent study [ 32 ] we studied the GroEL dependence of GFP, a eukaryotic protein that is often used as a fluorescent marker also in prokaryotic systems and folds in a GroE-dependent manner.…”
Section: Discussionsupporting
confidence: 94%
“…1990 genes) and compacted genome; all the WD40 proteins are detected by homology except TUP1. [41]. When I first made the CCT-WD40 connection it was obvious that the CCT ring had the required shape and dimensions to facilitate the binding of the propeller [2].…”
Section: -Bladed Wd40-repeat Protein Familymentioning
confidence: 99%
“…Plasmids were constructed using standard cloning procedures and Gibson assembly. Plasmids for reporter or microscopy assays: pBAD30-ibpA 5' UTR-gfp, pBAD30-gfp, pBAD30-ibpB 5' UTR-gfp, and pBAD30-ibpA 5' UTR-ibpA-gfp were constructed using DNA fragments amplified from pBAD30 [34], DNA fragments amplified from superfolder GFP [35], derived from a plasmid constructed previously [36], and DNA fragments amplified from E. coli genomic DNA. Plasmids for overexpression: pCA24N-rhodanese, pCA24N-rpoH, pCA24N-serA, pCA24N-gfp, pCA24N-ibpA, pCA24N-ibpA_AEA and pCA24N-ibpB were constructed using DNA fragments amplified from pCA24N [37], DNA fragments amplified from superfolder GFP [35,36] or DNA fragments amplified from E. coli genomic DNA.…”
Section: E Coli Strainsmentioning
confidence: 99%