Conversion of Existing AFLP Markers to SCAR Markers Linked to Globodera rostochiensis and Phytophthora infestans Resistance Could Be Performed Without Using Acrylamide Gel Electrophoresis

Ohbayashi, Kengo

doi:10.1007/s11540-021-09499-9

Cited by 2 publications

(1 citation statement)

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“…Molecular markers are extensively distributed in the genome of organisms and are abundant, easy to obtain, convenient to use, and inexpensive. These techniques, such as simple sequence repeats (SSR), have been extensively employed in population genetics investigations of plant pathogenic fungi [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Population Genetic Analysis of Phytophthora colocasiae from Taro in Japan Using SSR Markers

Zhang

Hieno

Otsubo

et al. 2023

JoF

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Phytophthora colocasiae is an important pathogen that causes great economic losses in taro production in tropical and subtropical regions, especially in Japan. Understanding the genetic variations in P. colocasiae populations and their transmission patterns in Japan is essential for effective disease control. Here, the genetic diversity of 358 P. colocasiae isolates, including 348 from Japan, 7 from China, and 3 from Indonesia, was assessed using 11 simple sequence repeat (SSR) primer pairs with high polymorphism. The phylogenetic tree of the SSR locus showed that the isolates from Japan could be divided into 14 groups, with group A being the dominant group. Among foreign isolates, only six from mainland China were similar to those from Japan and clustered in groups B and E. Analysis of molecular variance (AMOVA), principal components analysis (PCA), and cluster analysis (K = 3) results revealed a moderate level of genetic diversity, mainly within individuals. Populations showed high heterozygosity, a lack of regional differentiation, and frequent gene flow. Analysis of mating types and ploidy levels revealed that A2 and self-fertile (SF) A2 types and tetraploids were dominant across populations. Explanations and hypotheses for the results can provide more effective strategies for disease management of taro leaf blight.

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