Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

Davis, Reeta; Kataria, Rashmi; Cerrone, Federico; Woods, Trevor; Kenny, Shane T.; O’Donovan, Anthonia; Guzik, Maciej; Shaikh, Hamid; Duane, Gearóid; Gupta, Vijai Kumar; Tuohy, Maria G.; Padamatti, Ramesh Babu; Casey, Eoin; O’Connor, Kevin E.

doi:10.1016/j.biortech.2013.10.001

Cited by 145 publications

(79 citation statements)

References 31 publications

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“…[40][41][42][43][44][45][46][47][48][49][50] Production of mcl-PHAs and other interesting PHA copolymers including scl-copolymers and scl-mcl copolymers has also been reported using unconventional substrates, such as lignocellulosics e.g., grass mass hydrolysate, rice bran, rapeseed meal hydrolysate, and industrial waste e.g., whey, biodiesel waste, vinnase etc. 35,48,[51][52][53][54][55][56] Utilization of waste whey for PHA production is particularly useful as only 50 % of the whey produced is used for production of food ingredients and animal feed, while the rest remains as a pollutant owing to its high biological oxygen demand. Production of scl-mcl copolymer using whey adds additional merit, as such copolymers are natural blends that combine the interesting chemical, mechanical and thermal properties of both scl and mcl PHAs.…”

Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

“…However, quite interestingly, there have been only few reports on the utilization of pure or refined substrates (sugars) for P(3HB) production, [27][28][29][30] while different types of inexpensive carbon sources such as agro-industrial wastes including cane molasses, sugar beet juice, rice straw hydrolysate, grass biomass hydrolysate, plant oils e.g., coconut oil, have been largely investigated. [31][32][33][34][35][36][37][38] Alcaligenes and Bacillus sp. remain the microorganisms of choice for P(3HB) production (Table 1).…”

Section: Batch Fermentationmentioning

confidence: 99%

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Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

Section: Batch Fermentationmentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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“…H. mediterranei on hydrolyzed whey as substrate produced P(3HB:6 % 3HV), which improved to P(3HB:21.8 % 3HV:5.1 % 4HB) while the yield increased to 87.5 % when sodium valerate and c-butyrolactone was used to supplement the feed [38]. Pseudomonas can produce comparatively wider range of PHA on different wastes such as P(3HB:3HV) on whey [53], P(3HO:3HD:3HDD) on coprah oil [54], P(3HHx:3HO:3HD:3HDD:3HTD) on delignified rye grass hydrolysate [55] and P(3HHx:3HO:3HD:3HDD: 3HDDE:3HTD:3HTDE) on soy molasses [26]. As also observed with FAs, oily waste can provide a wider range of monomers in PHA [56,57].…”

Section: Substrate and Feeding Regimementioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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“…Bacteria of the genus Pseudomonas, which belongs to ribosomal RNA homology group I, are able to synthesize PHA using a variety of carbon sources, including glucose, fructose and sucrose (Diniz et al, 2004;Santhanam & Sasidharan, 2010;Davis et al, 2013), n-octane and octanoate (Smet et al, 1983;Durner et al, 2000), and fatty acids (Du & Yu, 2002;Allen et al, 2010), among others.…”

Section: Introductionmentioning

confidence: 99%

Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil

Silva

Antônio

Rossi

et al. 2014

Food Sci. Technol (Campinas)

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Pseudomonas oleovorans were grown on sugary cassava extracts supplemented with andiroba oil for the synthesis of a mediumchain-length polyhydroxyalkanoate (PHA MCL ). The concentration of total sugars in the extract was approximately: 40 g/L in culture 1, 15 g/L in cultures 2 and 3, and 10 g/L in culture 4. Supplementation with 1% andiroba oil and 0.2 g/L of (NH 4 ) 2 HPO 4 was performed 6.5 hours after growth in culture 3, and supplementation with the same amount of andiroba oil and 2.4 g/L of (NH 4 ) 2 HPO 4 was performed at the beginning of growth in culture 4. The synthesis resulted mainly in 3-hydroxy-decanoate and 3-hydroxy-dodecanoate units; 3-hydroxy-butyrate, 3-hydroxy-hexanoate; and 3-hydroxy-octanoate monomers were also produced but in smaller proportions. P. oleovorans significantly accumulated PHA MCL in the deceleration phase of growth with an oxygen limitation but with sufficient nitrogen concentration to maintain cell growth. The sugary cassava extract supplemented with andiroba oil proved to be a potential substrate for PHA MCL production.

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Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

Cited by 145 publications

References 31 publications

Strategies for Large-scale Production of Polyhydroxyalkanoates

Strategies for Large-scale Production of Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil

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