Conversion of myo-inositol-2-14C to labeled 4-O-methyl-glucuronic acid in the cell wall of maize root tips

Roberts, R. M.; Shah, Ramesh H.; Loewus, Frank A.

doi:10.1016/0003-9861(67)90499-7

Cited by 14 publications

(5 citation statements)

References 12 publications

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“…'Pectinase released 50 to 70 % of this into solution, distributed approximately 1:4 between acidic and neutral constituents. The elution profile of acidic constituents (fig 3) resembles an earlier one obtained from root tips that had been grown in sphagnum moss without attention to possible contamination (30). Separation and identification by paper chromatography of constituents from pooled fractions corresponding to 1P+2P, 3,P, 4P, and 5P confirmed this resemblance.…”

Section: Methodssupporting

confidence: 49%

“…Galacturonic acid was identified chemically by reduction with sodium borohydride to L-galactonate, lactonization to L-galactono-y-lactone, and conversion to i.-galactonamide (18). Chemical identification of 4-O-methyl-glucuronic acid was reported earlier (30).…”

Section: Methodsmentioning

confidence: 99%

“…Further separation of constituents in the neutral effluent and in pooled fractions of the acidic eluate was accomplished by paper chromatography (30) (30). Glucuronic acid was identified chemically by reduction with sodium borohydride to L-gulonate, lactonization to L-gulono-y-lactone.…”

Section: Methodsmentioning

confidence: 99%

“…A prelimiinar-report on I portion of this work dealing with the conversion of mvtyo-inositol-2-' 4C to 4-0-nmethvl-glucuronic acid units of cell wall polysaccharides in Zea wilays root tips has appeared (30 ) 4. Another paper, in which the isolation and identification of labeled constituents in the soluble portion of Zea root tips labeled with myo-inositol-2-14C is reported, is in preparation (J. Deshusses and F. Loewus, unpublished).…”

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confidence: 99%

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Inositol Metabolism in Plants. V. Conversion of Myo-inositol to Uronic Acid and Pentose Units of Acidic Polysaccharides in Root-tips of Zea mays

Roberts¹,

Deshusses²,

Loewus³

1968

Plant Physiol.

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Abstract. The metabolism of myo-inositol-2-14C, D-glucuronate-1-14C, D-glueuronate-6-14C, and L-methionine-methyl-14C to cell wall polysaccharides was investigated in excised root-tips of 3 day old Zea mays seedlings. From myo-inositol, about one-half of incorporated label was recovered in ethanol insoluble residues. Of this label, about 90 % was solubilized by treatment, first with a preparation of pectinase-EDTA, then with dilute hydrochloric acid. The only labeled constituents in these hydrolyzates were D-galacturonic acid, D-glucuronic acid, 4-0-methyl-D-glucuronic acid, D-xylose, and L-arabinose, or larger oligosaccharide fragments containing these units. Medium external to excised root-tips grown under sterile conditions in myo-inositol-2-14C contained labeled polysaccharide.When label was supplied in the form of D-glucuronate, the pattern of labeled uronic acid and pentose units in cell wall polysaccharides resembled that obtained from labeled myr.-inositol, indicating that both substances were metabolized along a common path during polysaccharide formation, and that methylation occurred at a step subsequent to uronic acid formation. When label was supplied in the form of L-methionine-methyl-14C, 4-0-methyl-D-glucuronic acid was the only labeled monosaccharide component that survived enzymatic or acid hydrolysis.Zea mays endosperm, a known source of phytin, developed maximal phytase activity after the third day of germination. Results obtained here suggest that myo-inositol released by hydrolysis of phvtin represents the initial precursor of a normal, possibly predominant pathway for the formation of uronic acids in plants. Galacturonic3, glucuronic3, and 4-0-methvl-glucuronic acid3 constitute the 3 major acidic glvcosyl components of polvsaccharides in cell walls of higher plants. Galacturonic acid, usually

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Section: Methodssupporting

confidence: 49%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Inositol Metabolism in Plants. V. Conversion of Myo-inositol to Uronic Acid and Pentose Units of Acidic Polysaccharides in Root-tips of Zea mays

Roberts¹,

Deshusses²,

Loewus³

1968

Plant Physiol.

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“…If the same acidic mixture was separated on paper with solvent E, a system that resolves glucuronic acid and 4-0-methyl glucuronic acid, three radioactive peaks were obtained in a radiochromatogram scan, of which the fastest was a mixture of glucuronic and galacturonic acids. The latter two are not resolved by this solvent system (28). No radioactivity was detected in the region of 4-0-methyl glucuronic acid (Rglucuronic acid =2.0).…”

Section: Materlil and Methodsmentioning

confidence: 83%

The Composition of Stigmatic Exudate from Lilium longiflorum

Labarca¹,

Kroh²,

Loewus³

1970

Plant Physiol.

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Stigmatic exudate, a secretion product recovered from the upper surface of Lilium longiflorum pistils, has been examined. Over 99% of the exudate is accounted for as water, carbohydrate, and protein. Exclusive of water, 95% is a high molecular weight, protein-containing polysaccharide composed of galactose, arabinose, rhamnose, glucuronic acid, and galacturonic acid.Detached pistils supplied with myo-inositol-U-14C, myoinositol-2-3H, D-glucose-1-14C, or L-proline-U-14C produce labeled stigmatic exudate. When myo-inositol is supplied, the exudate is rich in labeled arabinose and uronic acids, but some label also recycles through the hexose phosphate pool of secreting cells, causing label to appear in galactose and rhamnose residues. When glucose is provided, galactose is the major constituent labeled but all of the other carbohydrate constituents are also labeled. Proline produces a pattern very similar to that obtained with glucose.

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Inositol and Plant Cell Wall Polysaccharide Biogenesis

Loewus

Subcellular Biochemistry

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Conversion of myo-inositol-2-14C to labeled 4-O-methyl-glucuronic acid in the cell wall of maize root tips

Cited by 14 publications

References 12 publications

Inositol Metabolism in Plants. V. Conversion of Myo-inositol to Uronic Acid and Pentose Units of Acidic Polysaccharides in Root-tips of Zea mays

Inositol Metabolism in Plants. V. Conversion of Myo-inositol to Uronic Acid and Pentose Units of Acidic Polysaccharides in Root-tips of Zea mays

The Composition of Stigmatic Exudate from Lilium longiflorum

Inositol and Plant Cell Wall Polysaccharide Biogenesis

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