Conversion of plasma progesterone to deoxycorticosterone in men, nonpregnant and pregnant women, and adrenalectomized subjects.

Abstract

A B S T R A C T During the third trimester of

Origin of urinary nonconjugated 19-nor-deoxycorticosterone and metabolism of infused radiolabeled 19-nor-deoxycorticosterone in men and women.

“…[l,2(N-3H]19-nor-DOC (50 Ci/mmol) was synthesized as described (12) and purified by high performance liquid chromatography (HPLC) and by gradient-elution column chromatography on celite-ethylene glycol (13). ["4CIDOC (50 mCi/mmol), purchased from New England Nuclear (Boston, MA), was purified as described (13).…”

“…[l,2(N-3H]19-nor-DOC (50 Ci/mmol) was synthesized as described (12) and purified by high performance liquid chromatography (HPLC) and by gradient-elution column chromatography on celite-ethylene glycol (13). ["4CIDOC (50 mCi/mmol), purchased from New England Nuclear (Boston, MA), was purified as described (13). A mixture of a tracer dose that consisted of ['4C]DOC (-18 gCi) and [3H] 19-nor-DOC (-7 uCi) in 4.5 ml NaCl (0.15 M) and 0.5 ml ethanol was infused intravenously into two healthy, ambulatory men, ages 47 and 52, and into two presumably ovulatory women, ages 33 and 35; thereafter, urine was collected from each subject for 72 h. Nonradiolabeled 19-nor-DOC was prepared as described (5) and the authenticity of this compound was validated by use of gas chromatography-mass spectrometry.…”

“…Radiolabeled steroids in these residues were isolated by successive chromatographic procedures that included, initially, gradient-elution column chromatography on celite-ethylene glycol (13 By gradient-elution column chromatography, we isolated five major peaks of carbon-14 radioactivity and six peaks that contained tritium radioactivity. In the case of the carbon-14 peaks, there were corresponding peaks of tritium that were slightly more polar than the carbon-14 counterpart (Fig.…”

“…Recently, we found that in the human, DOC is formed in extraadrenal sites from plasma progesterone (13)(14)(15). Some extraadrenal tissues of DOC formation, e.g., kidney and aorta, are believed to be target tissues of DOC action (16)(17)(18)(19).…”

Trend Analysis of Serum Progesterone, Deoxycorticosterone, Deoxycorticosterone Sulfate, Cortisol, Corticosterone, 18-Hydroxydeoxycorticosterone and Estradiol in Early Neonates.

“…Some investigators observed in vitro, using previable fetus or fetal adrenal tissue perfused with labeled progesterone, that DOC sulfate was formed from progesterone at the adrenal gland (Bird et al, 1965;Klein etal., 1965). However, recent in vitro studies have demonstrated that steroid 21-hydroxylase and 21-hy-droxy-steroid sulfotransferase are present in extraadrenal tissues (Ottoson et al, 1984;Winkel et al, 1980;Casey et al, 1983;Casey et al, 1981). Extraadrenal production as the main site of these steroids was suggested by many authors (Pakravan et al, 1974;Dorr et al, 1986;Parker et al, 1983 b;Craig et al, 1980).…”