1984
DOI: 10.1128/mcb.4.6.1141
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Conversion through homologous recombination of the gene encoding Simian virus 40 115,000-molecular-weight super T antigen to a gene encoding a normal-size large T antigen variant.

Abstract: We have previously cloned the gene encoding a 115,000-Mr super T antigen (115K super T antigen), an elongated form of the Simian virus 40 large T antigen, originating from the rat cell line V 11 Fl clone 1, subclone 7 (May et al., J. Virol. 45:901-913, 1983). DNA sequence analysis has shown that the 115K super T antigen gene contains notably an in-phase duplication of a sequence located in the region of tsA mutations. We have also shown that the 115K super T antigen gene is able to induce the formation of tran… Show more

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Cited by 9 publications
(3 citation statements)
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References 47 publications
(28 reference statements)
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“…The evidence for recombination between duplicated segments in SVT2 is the appearance of 94-kDa wild-type large T antigen in extended cell culture, correlated with spontaneous deletion of the 1.8-kb duplicated segment in the coding sequence (15). The same phenomenon, disappearance of a super T antigen correlated with deletion of a duplicated segment, was also observed in an SV40-transformed rat line (49). Despite the widespread duplications in integrated viral sequences in SV40-transformed cells and demonstrated deletions of duplications, it is not yet known whether recombination between duplications is the major mechanism of rearrangement of integrated SV40 DNA.…”
mentioning
confidence: 56%
See 1 more Smart Citation
“…The evidence for recombination between duplicated segments in SVT2 is the appearance of 94-kDa wild-type large T antigen in extended cell culture, correlated with spontaneous deletion of the 1.8-kb duplicated segment in the coding sequence (15). The same phenomenon, disappearance of a super T antigen correlated with deletion of a duplicated segment, was also observed in an SV40-transformed rat line (49). Despite the widespread duplications in integrated viral sequences in SV40-transformed cells and demonstrated deletions of duplications, it is not yet known whether recombination between duplications is the major mechanism of rearrangement of integrated SV40 DNA.…”
mentioning
confidence: 56%
“…Possible molecular mechanism of spontaneous rearrangement of integrated SV40 DNA. The molecular mechanism of rearrangement of SV40 DNA is not yet known, but it is possible that T-antigen binding to the integrated SV40 origin of DNA replication is responsible for rearrangements, as it could be in producing rearrangements in extrachromosomal SV40 sequences during transfection (16,49). Alternatively, the cellular sites of integration of SV40 DNA might be intrinsically unstable for unknown reasons.…”
mentioning
confidence: 99%
“…Super T-Ag may be generated as a result of internal in-phase duplications in the coding region of the T-Ag gene (20)(21)(22), with the duplicated sequence corresponding to the region of the SV40 genome to which many of the tsA mutations have been mapped (17), or by differential splicing between two integrated partial copies of the viral genome (19). Rearrangements of integrated viral sequences can occur in SV40-transformed cells after the initial integration event (1,2,8,12,14,25,26) or soon after infection, before viral DNA integration (19,24). Some such rearrangements result in the capacity to generate super T-Ag (if a functional SV40 origin of replication is present [9]).…”
Section: -mentioning
confidence: 99%