Converting solution viscosity to distance-readout on paper substrates based on enzyme-mediated alginate hydrogelation: Quantitative determination of organophosphorus pesticides

Xu, Jianling; Hu, Xiaotong; Khan, Hizbullah; Tian, Miaomiao; Yang, Li

doi:10.1016/j.aca.2019.04.017

Cited by 25 publications

(10 citation statements)

References 39 publications

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“…First, we use biologically derived materials (i.e., the polysaccharides agarose and chitosan) for the fabrication of functional films. Increasingly, bio-derived materials are being investigated for the creation of high-performance materials that are expected to be environmentally friendly, biocompatible, and potentially even edible. ,− Second, we enlist the unique properties of catechol to report the activities of an enzymatic redox reaction through catechol’s redox-state-dependent optical properties. Catechols (including catechols from foods) are gaining importance in functional materials because they have two stable redox states (Q and QH 2 ) that enable them to serve as bistable switches and molecular memory devices for bioelectronics applications , and can exchange electrons with common biological oxidants and reductants providing a means to communicate with biology through its native redox modality. , Thus, controlling the properties of polysaccharide-based materials and the redox reactions of phenolics may be integral not only for functional foods but also for sustainable high-performance materials.…”

Section: Discussionmentioning

confidence: 99%

Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging

Rzasa

Kim

et al. 2021

ACS Sustainable Chem. Eng.

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Biology provides an array of materials and mechanisms to create sustainable high-performance materials. Here, we prepared a polysaccharide film and patterned it with redox-active catechol moieties using a biomimetic oxidative grafting method. In this proof-of-concept study, we show that the film allows results from an enzymatic biosensing reaction to be reported through cell phone imaging for the detection of glucose in food and beverage products (glucose is a marker for high-fructose corn syrup; HFCS). This method uses glucose dehydrogenase (GDH) to confer molecular recognition, while the NADH product transfers its electrons to catechol moieties that are patterned onto a polysaccharide film. The “accumulation” of electrons by the catechol pattern can be detected because the oxidized quinone state is darker in color than the reduced catechol state. Using a commercially available cell phone imaging app, we demonstrate that HFCS-containing products can be distinguished from analogous products containing either sucrose or artificial sweeteners. Thus, we envision that this method could allow buyers or consumers to detect glucose on-site or in-home. In addition, this work further illustrates the potential of catechol-based materials for performing redox-based functions.

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Section: Discussionmentioning

confidence: 99%

Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging

Rzasa

Kim

et al. 2021

ACS Sustainable Chem. Eng.

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“…The µPAD has been applied in healthcare diagnostics [ 2 , 3 ], food quality testing, and environmental monitoring [ 4 , 5 , 6 , 7 ]. In terms of pesticide analysis, organophosphate and carbamate can also be analyzed on a µPAD using chemicals and nanoparticles [ 8 , 9 , 10 ], targeting the inhibition of acetylcholinesterase (AChE) activity by the pesticides for the detection principle [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Paper-Based Analytical Device for Type-II Pyrethroid Targets in an Environmental Water Sample

Pengpumkiat

Nammoonnoy²,

Wongsakoonkan

et al. 2020

Sensors

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A detection method for type-II pyrethroids in an environmental water sample using a microfluidic paper-based analytical device (µPAD) is reported here. The detection approach is based on the formation of cyanide from the hydrolysis of type-II pyrethroids and the colorimetric detection of cyanide on a layer-based µPAD. Parafilm and inexpensive laminating pouches were used to create a hydrophobic barrier for the assay on the µPAD. This detection approach was selective to type-II pyrethroids in water for which an environmental water sample was tested. The calibration curves for cypermethrin, deltamethrin, cyhalothrin, and fenvalerate ranged from 2 to 40 µg/mL without sample preconcentration. The lower concentrations of type-II pyrethroids can be assessed by including a preconcentration step prior to the detection on a µPAD. This detection system provides an alternative platform for fast, semiquantitative testing for pesticide contamination in environmental surface water by allowing for portability, low reagent/sample consumption, and low-cost testing.

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“…The detection of pesticides, heavy-metal ions, and other biological molecules has been demonstrated by the hydrogelation of alginate. For instance, organophosphorus pesticides can be detected based on the migration distance of alginate hydrogel on paper . Previous work has suggested that enzymes such as alkaline phosphatase (ALP) could trigger the hydrosol–hydrogel transition.…”

mentioning

confidence: 99%

“…For instance, organophosphorus pesticides can be detected based on the migration distance of alginate hydrogel on paper. 25 Previous work 26 has suggested that enzymes such as alkaline phosphatase (ALP) could trigger the hydrosol−hydrogel transition. This sol−gel transition can result in a change in the diffusion rate of water protons that leads to a change in the T 2 signal.…”

mentioning

confidence: 99%

Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels

et al. 2021

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In this work, we develop a direct transverse relaxation time (T 2) biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol–gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a T 2 change of water molecules. By means of enzyme-modulated sol–gel transition and antigen–antibody interactions, this T 2 biosensor displays high sensitivity for detecting 50 CFU/mL S. enteritidis within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection.

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Converting solution viscosity to distance-readout on paper substrates based on enzyme-mediated alginate hydrogelation: Quantitative determination of organophosphorus pesticides

Cited by 25 publications

References 39 publications

Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging

Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging

A Microfluidic Paper-Based Analytical Device for Type-II Pyrethroid Targets in an Environmental Water Sample

Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels

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