Cooking with Active Oxygen and Solid Alkali: A Promising Alternative Approach for Lignocellulosic Biorefineries

Jiang, Yetao; Zeng, Xianhai; Luque, Rafael; Tang, Xing; Sun, Yong; Lei, Tingzhou; Liu, Shijie

doi:10.1002/cssc.201700906

Cited by 40 publications

(11 citation statements)

References 47 publications

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“…Cornstalk, bamboo and reed as non-wood and non-food biomass materials, depending on their different fiber processing technology, can turn them into glucose and other wide range of products, which has been widely used in China, the United States and some of European countries [ 37 ]. Currently, T. reesei Rut-C30 (TR) is recognized as an excellent cellulase-producing strain.…”

Section: Resultsmentioning

confidence: 99%

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Peng

Lin

et al. 2021

Biotechnol Biofuels

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Background Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase production, which are far from meeting the requirements of industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy is an effective method to increase the production of fungal enzymes. Results This study obtained a mutant of Trichoderma afroharzianum by exposures to N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric and Room Temperature Plasma (ARTP) and ALE with high sugar stress. The T. afroharzianum mutant MEA-12 produced 0.60, 5.47, 0.31 and 2.17 IU/mL FPase, CMCase, pNPCase and pNPGase, respectively. These levels were 4.33, 6.37, 4.92 and 4.15 times higher than those of the parental strain, respectively. Also, it was found that T. afroharzianum had the same carbon catabolite repression (CCR) effect as other Trichoderma in liquid submerged fermentation. In contrast, the mutant MEA-12 can tolerate the inhibition of glucose (up to 20 mM) without affecting enzyme production under inducing conditions. Interestingly, crude enzyme from MEA-12 showed high enzymatic hydrolysis efficiency against three different biomasses (cornstalk, bamboo and reed), when combined with cellulase from T. reesei Rut-C30. In addition, the factors that improved cellulase production by MEA-12 were clarified. Conclusions Overall, compound mutagenesis combined with ALE effectively increased the production of fungal cellulase. A super-producing mutant MEA-12 was obtained, and its cellulase could hydrolyze common biomasses efficiently, in combination with enzymes derived from model strain T. reesei, which provides a new choice for processing of bioresources in the future.

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Section: Resultsmentioning

confidence: 99%

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Peng

Lin

et al. 2021

Biotechnol Biofuels

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“…authors' group 55 . The bamboo pulp was reformed by mechanical treatment and used as the original cellulose fiber, which has been described in detail in our earlier work 42,43 .…”

Section: Methodsmentioning

confidence: 99%

Interfacial assembly of self-healing and mechanically stable hydrogels for degradation of organic dyes in water

et al. 2020

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Mussel-inspired hydrogels have gained attention for underwater applications, including treatment of wastewater. However, they are typically limited by poor mechanical properties, short-term mechanical stability and by not being reusable. Here, we develop a mechanically stable and self-healing hydrogel with high mechanical strength for the degradation of dyes in wastewater, based on cellulose-derived co-polydopamine@Pd nanoparticles. A dynamic catechol redox system was achieved by reversible conversion between semiquinone and quinone/hydroquinone radicals, endowing the hydrogel with stable mechanical properties and self-healing behavior. Furthermore, a graphene oxide membrane is covalently grafted on to the hydrogel surface, which regulates its water permeability and intercepts some metal ions or large particles, protecting the hydrogel structure. High catalytic activity for anionic and cationic dyes is achieved, with the degradation rate reaching more than 95% after multiple cycles without significant deterioration in performance or hydrogel structure. Our work demonstrates a route to achieve mechanically stable hydrogels for degradation of organic dyes in wastewater.

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“…In fact, the advantage of sodium hydroxide pretreatment can break down effectively the internal structure of lignocellulose component through degrading the ester and glycosidic chains. In addition, the alkali can alter the structure of lignin, causing cellulose swelling and partial decrystallization of cellulose [16]. Moreover the safer handling and the lower toxicity in the pretreatment, including lower operating temperatures and faster process times, make it an attractive alternative to ammonia pretreatment [17].…”

Section: Batch Simultaneous Saccharification and Fermentation (Ssf) Fmentioning

confidence: 99%

Evaluation of Alkali-Pretreated Soybean Straw for Lignocellulosic Bioethanol Production

Kim

2018

International Journal of Polymer Science

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Soybean straw is a renewable resource in agricultural residues that can be used for lignocellulosic bioethanol production. To enhance enzymatic digestibility and fermentability, the biomass was prepared with an alkali-thermal pretreatment (sodium hydroxide, 121°C, 60 min). The delignification yield was 34.1~53%, in proportion to the amount of sodium hydroxide, from 0.5 to 3.0 M. The lignin and hemicellulose contents of the pretreated biomass were reduced by the pretreatment process, whereas the proportion of cellulose was increased. Under optimal condition, the pretreated biomass consisted of 74.0±0.1% cellulose, 10.3±0.1% hemicellulose, and 10.1±0.6% lignin. During enzymatic saccharification using Cellic® CTec2 cellulase, 10% (w/v) of pretreated soybean straw was hydrolyzed completely and converted to 67.3±2.1 g/L glucose and 9.4±0.5 g/L xylose with a 90.9% yield efficiency. Simultaneous saccharification and fermentation of the pretreated biomass by Saccharomyces cerevisiae W303-1A produced 30.5±1.2 g/L ethanol in 0.5 L fermented medium containing 10% (w/v) pretreated biomass after 72 h. The ethanol productivity was 0.305 g ethanol/g dry biomass and 0.45 g ethanol/g glucose after fermentation, with a low concentration of organic acid metabolites. Also, 82% of fermentable sugar was used by the yeast for ethanol fermentation. These results show that the combination of alkaline pretreatment and biomass hydrolysate is useful for enhancing bioethanol productivity using delignified soybean straw.

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Cooking with Active Oxygen and Solid Alkali: A Promising Alternative Approach for Lignocellulosic Biorefineries

Cited by 40 publications

References 47 publications

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Interfacial assembly of self-healing and mechanically stable hydrogels for degradation of organic dyes in water

Evaluation of Alkali-Pretreated Soybean Straw for Lignocellulosic Bioethanol Production

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