Cooperation across the histocompatibility barrier: H2d T cells primed to antigen in an H-2d environment can cooperate with H-2k B cells.

Abstract

H-2d spleen cells derived from either tetraparental or semiallogeneic radiation bone marrow chimeras can be primed to antigen within H-2d recipients to generate helper T cells capable of cooperating in a secondary response with equal efficiency with H-2d or H-2k B cells. Thus it would seem that the cooperative act between T and B cells does not require that the T cell interacts with its target B cells by either cell interaction genes or via an altered self mechanism involving both antigen and the target B-cell I-region products. This does not preclude a requirement for associative recognition or altered self in the interaction of helper T cells with accessory cells.

Conditions Determining the Generation and Expression of T Helper Cells

“…(Table X) that when antigen-primed helper T cells were derived from TBMC then these cooperated most efficiently with histoincompatible B cells, thus apparently excluding a requirement for T and B cells to share matching Cell Interartion gene products. For example, (Table XI) when we assayed the cooperative function of Summary of data of Waldmann et al (1976d). More recently similar results have been obtained with H-2'' T cells derived from H-2'' + H-2^ TBMC, which were able to cooperate effectively with H-2B cells (Table XI).…”

“…When this experiment was performed it was found that H-2'' spleen T cells derived from chimeric (H-2'' -I-H-2*') mice could, after priming in an H-2'' environment still cooperate efficiently with both H-2'' and H-2^ B cells (Waldmann et al 1976d). If this were the simple explanation then one would predict that if chimeric T cells of one parental type (say H-2'') were transferred into syngenic (H-2'^) recipients and primed there for the first time, then these cells should cooperate only with H-Z", and not H-2^ B cells.…”

“…It is interesting that the situation can be somewhat different when T cells from single chimeras instead of double chimeras (TBMC) are used as sources of helper cells. For example, (Table XI) when we assayed the cooperative function of Summary of data of Waldmann et al (1976d). T cells were obtained from primed conventional mice or from chimeric (TMBC) mice.…”

The Influence of the Major Histocompatibility Complex on the Function of T‐Helper Cells in Antibody Formation

“…First, the in vivo studies with Fj -> BIO and Fj ->-AJ chimeras showed that T memory cells from those animals still showed haplotype preferences, even when transferred to and primed in (BIO X AJ) Fj irradiated recipients where an abundance of Fj macrophages are available for antigen presentation. Second, our previous studies with this in vitro system suggest that T-helper cells are monogamous, i. e. help one or very few B cells (Waldmann et al, 1976, Phillips & Waldmann, 1977. This finding is incompatible with any model that implies that T helper memory cells are activated by antigen presenting macrophages to release widely diffusible specific helper factors, but rather emphasises the need for T-and B-cells to be close if not in actual contact, to achieve cooperation.…”

“…Thelper cell function was then assessed 7 days later on A and B B-cells. This sort of experiment had given somewhat ambiguous results in our earlier studies (Waldmann et al, 1976). In brief, we had observed that Balb/c spleen cells from Balb/c -^ (Balb/c X C3H/He) F^ single or Balb/c + C3H/He ^ (Balb/c X C3H/He) Fj double chimeras could, after adoptive priming in a syngeneic (Balb/c) environment, cooperate with both Balb/c and CBA B-cells.…”