Cooperative Effects of Q10, Vitamin D&lt;sub&gt;3&lt;/sub&gt;, and &lt;smlcap&gt;L&lt;/smlcap&gt;-Arginine on Cardiac and Endothelial Cells

Molinari, Claudio; Morsanuto, Vera; Polli, Simona; Uberti, Francesca

doi:10.1159/000484928

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…Nitric oxide (NO) production by astrocyte was assessed by indirect measurement of nitrite concentration using Griess assay (Promega Corporation, Madison, WI, USA) following the manufacturer’s instruction, as previously described [ 67 ]. The absorbance was measured by a microplate reader (VICTOR X4 multilabel plate reader, Perkin Elmer, Waltham, MA, USA) at 550 nm.…”

Section: Methodsmentioning

confidence: 99%

A New Palmitoylethanolamide Form Combined with Antioxidant Molecules to Improve Its Effectivess on Neuronal Aging

et al. 2020

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Palmitoylethanolamide is a nutraceutical compound naturally produced in many plants and animal source foods, but the natural form is poorly water-soluble. It has demonstrated an anti-inflammatory role as a neuroprotective mediator, acting on several molecular targets of the central nervous system involved on brain aging process. In healthy adults, palmitoylethanolamide is an endogenous PPAR-α (peroxisome proliferator-activated receptor α) agonist through which it performs anti-inflammatory activity and provides its effects by activating the cannabinoid receptor. The different formulations of palmitoylethanolamide (micronized palmitoylethanolamide, FM-LipoMatrix® palmitoylethanolamide and FM-LipoMatrix® palmitoylethanolamide plus lipoic acid and vitamin D3) were analyzed starting from intestinal barrier, to verify their bioavailability, to in primary astrocytes in which cell viability, reactive oxygen species (ROS) and nitric oxide (NO) production, NFKB activity, MAPK, p53 and PPARα activities were investigated. Additionally, cannabinoid and estrogen receptors were analyzed using the western blot technique. The combination of palmitoylethanolamide in FM-LipoMatrix®, lipoic acid and vitamin D3 shows better absorption predicting an improvement on plasma concentration; this formulation also shows a reduction in ROS and NO production and the data show the interaction of palmitoylethanolamide with cannabinoids and estrogen receptors inhibiting neuroinflammatory markers. All these data support the hypothesis of a new potential strategy to restore brain function and slow down brain aging in humans.

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Section: Methodsmentioning

confidence: 99%

A New Palmitoylethanolamide Form Combined with Antioxidant Molecules to Improve Its Effectivess on Neuronal Aging

et al. 2020

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“…Vitamin D can affect blood pressure through several mechanisms. Indeed, in both animals and humans it has been shown that vitamin D decreases renin-angiotensin-aldosterone system (RAAS) activity (Li et al, 2002 ; Tomaschitz et al, 2010 ), modulates endothelial function (Wong et al, 2010 ; Pittarella et al, 2015 ; Molinari et al, 2018 ) and regulates vascular oxidative stress (Argacha et al, 2011 ).…”

Section: The Antioxidant Therapy In Hypertensionmentioning

confidence: 99%

The Antioxidant Therapy: New Insights in the Treatment of Hypertension

et al. 2018

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Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a key role in the regulation of the physiological and pathological signaling within the vasculature. In physiological conditions, a delicate balance between oxidants and antioxidants protects cells from the detrimental effects of ROS/RNS. Indeed, the imbalance between ROS/RNS production and antioxidant defense mechanisms leads to oxidative and nitrosative stress within the cell. These processes promote the vascular damage observed in chronic conditions, such as hypertension. The strong implication of ROS/RNS in the etiology of hypertension suggest that antioxidants could be effective in the treatment of this pathology. Indeed, in animal models of hypertension, the overexpression of antioxidants and the genetic modulation of oxidant systems have provided an encouraging proof of concept. Nevertheless, the translation of these strategies to human disease did not reach the expected success. This could be due to the complexity of this condition, whose etiology depends on multiple factors (smoking, diet, life styles, genetics, family history, comorbidities). Indeed, 95% of reported high blood pressure cases are deemed “essential hypertension,” and at the molecular level, oxidative stress seems to be a common feature of hypertensive states. In this scenario, new therapies are emerging that could be useful to reduce oxidative stress in hypertension. It is now ascertained the role of Vitamin D deficiency in the development of essential hypertension and it has been shown that an appropriate high dose of Vitamin D significantly reduces blood pressure in hypertensive cohorts with vitamin D deficiency. Moreover, new drugs are emerging which have both antihypertensive action and antioxidant properties, such as celiprolol, carvedilol, nebivolol. Indeed, besides adrenergic desensitization, these kind of drugs are able to interfere with ROS/RNS generation and/or signaling, and are therefore considered promising therapeutics in the management of hypertension. In the present review we have dealt with the effectiveness of the antioxidant therapy in the management of hypertension. In particular, we discuss about Vitamin D and anti-hypertensive drugs with antioxidant properties.

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“…Beyond the above-cited experimental and clinical reports, the extensive literature discusses the potential benefits of treating ARD and mitochondria-defective diseases using MNs [ 14 , 25 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 ]. Consistent with the available database from pre-clinical and clinical investigations, the available MDF-related reviews mainly focus on the potential advantages of using individual MNs in the treatment of mitochondria-defective disorders, with the exception of the “mitochondrial cocktail” perspective proposed by Tarnopolsky [ 136 ] and as discussed in our previous review [ 137 ].…”

Section: Discussionmentioning

confidence: 99%

Aging-Related Disorders and Mitochondrial Dysfunction: A Critical Review for Prospect Mitoprotective Strategies Based on Mitochondrial Nutrient Mixtures

Pagano

Pallardó

Lyakhovich

et al. 2020

IJMS

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A number of aging-related disorders (ARD) have been related to oxidative stress (OS) and mitochondrial dysfunction (MDF) in a well-established body of literature. Most studies focused on cardiovascular disorders (CVD), type 2 diabetes (T2D), and neurodegenerative disorders. Counteracting OS and MDF has been envisaged to improve the clinical management of ARD, and major roles have been assigned to three mitochondrial cofactors, also termed mitochondrial nutrients (MNs), i.e., α-lipoic acid (ALA), Coenzyme Q10 (CoQ10), and carnitine (CARN). These cofactors exert essential–and distinct—roles in mitochondrial machineries, along with strong antioxidant properties. Clinical trials have mostly relied on the use of only one MN to ARD-affected patients as, e.g., in the case of CoQ10 in CVD, or of ALA in T2D, possibly with the addition of other antioxidants. Only a few clinical and pre-clinical studies reported on the administration of two MNs, with beneficial outcomes, while no available studies reported on the combined administration of three MNs. Based on the literature also from pre-clinical studies, the present review is to recommend the design of clinical trials based on combinations of the three MNs.

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Cooperative Effects of Q10, Vitamin D<sub>3</sub>, and <smlcap>L</smlcap>-Arginine on Cardiac and Endothelial Cells

Cited by 14 publications

References 48 publications

A New Palmitoylethanolamide Form Combined with Antioxidant Molecules to Improve Its Effectivess on Neuronal Aging

A New Palmitoylethanolamide Form Combined with Antioxidant Molecules to Improve Its Effectivess on Neuronal Aging

The Antioxidant Therapy: New Insights in the Treatment of Hypertension

Aging-Related Disorders and Mitochondrial Dysfunction: A Critical Review for Prospect Mitoprotective Strategies Based on Mitochondrial Nutrient Mixtures

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